FastQCFastQC Report
Wed 27 Sept 2023
ERR3403487_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3403487_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19420244
Total Bases990.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1651510.8504064109596152TruSeq Adapter, Index 12 (100% over 49bp)
AAGACTATACTTTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTT311340.1603172442117617No Hit
ATACTTACCTGGCAGGGGAGATACCATGATCACGAAGAGATCGGAAGAGC247990.12769664480013743No Hit
ATACTTACCTGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAG220610.11359795479397684No Hit
ATACTTACCTGGCAGGGGAGATACCATGATCACGAAAGATCGGAAGAGCA200740.1033663634710254No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)