FastQCFastQC Report
Wed 27 Sept 2023
ERR3403489_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3403489_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28383896
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA7368482.5960072570728134No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGACGTAGATCGGAAGAGCAC5035371.7740235519464982No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC2437370.8587158013825869No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG2191800.7721984325196232No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA2017770.7108854964801168No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT2015100.7099448222330014No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAA1999560.7044698867273189No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1795770.6326721321132237Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT1460490.5145488131720889No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1414880.4984798422316654No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA1412230.4975462142335922No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC1255560.44234942236259606No Hit
GTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGAGATCGGAAGAGCACAC1192350.42007975226515765No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1072540.37786919737868263TruSeq Adapter, Index 4 (100% over 49bp)
TCGACTGCATAATTTGTGGTAGTGGGGGACTGCGTAGATCGGAAGAGCAC1060430.37360269358371384No Hit
AAAACATCACCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCAC871570.30706496387951815No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGT862130.30373913433166466No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT845990.2980528113547203No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGC774590.2728977022745574No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAG772650.2722142161174773No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG762070.26848675037422626No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGAGATCGGAAGAGCAC705000.2483802787327011No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGTAGATCGGAAGAGCA679600.23943154244928183No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAG672590.2369618321600389No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG670070.23607400478073906No Hit
GCTTAACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGC666550.23483386494933609No Hit
CTTAACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCA664200.2340059306868937No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT622490.2193109783096725No Hit
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGT610710.21516073762389773No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC610680.21515016825033462Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGACCTCATAACCCAATTCAGACTACTCTCCTCCGCCAGATCGGAAGA605430.2133005278767932No Hit
ACGATCAAAAGGAACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACAC592260.2086605728825951No Hit
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCCAGATCGGAAGAGCACACG571480.20133952012789222No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG567700.20000777905894246No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG549720.1936732011701283No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC543420.19145363272187862No Hit
CGATCAAAAGGAACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACACG536670.18907552367018254No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT533110.18782129134069545Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA529720.18662695212806588No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGT523140.18430873619322732No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA507830.1789148325515285No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG493560.17388733386001695No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC490270.1727282258925977No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT486550.17141762357077409No Hit
AGTCGGCACTGGCAATTTTTGACAGTCTCTACGGAGACTGAGATCGGAAG479780.16903246827003593No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGCGAGATCGGAAGAGCACA467580.16473425635437786No Hit
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGAGCAGATCGGAAGAGCACA464510.16365265712642127No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAG459350.16183472487356915No Hit
AAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAGC448310.1579451954023507No Hit
TGCTGAAGGCCTGTATCCTAGGCTACACACTGAGGACAGATCGGAAGAGC447770.157754946678215No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG444960.15676494868780524No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC436360.1537350615997184No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC432020.15220602555759083No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG419600.14783030490247007No Hit
TCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC407820.14368006421669527No Hit
GGGGACCTCATAACCCAATTCAGACTACTCTCCTCCGCCCAGATCGGAAG394110.13884986049836145No Hit
TCCTTAGGTCGCTGGTTCGATTCCGGCTCGAAGGAGATCGGAAGAGCACA390910.1377224606516315No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG382720.1348370216689069No Hit
ATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAGCAC381390.13436844610760976No Hit
TCCTTAGGTCGCTGGTTCGAATCCGGCTCGAAGGAGATCGGAAGAGCACA380850.13417819738347408No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGA380580.13408307302140623No Hit
CGACTGCATAATTTGTGGTAGTGGGGGACTGCGTAGATCGGAAGAGCACA376550.13266325383943064No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACAC372010.13106375530688247No Hit
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCAGATCGGAAGAGCACAC365910.12891464934905342No Hit
AAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGATCGGAAGAGCAC365640.12881952498698557No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACA364720.1284953975310507No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAGATCGGAAGAGC363350.12801272947166942No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCAGATCGGAAGAGCACAC359520.12666337278011447No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA341540.12032879489130034No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG336190.11844392327254863No Hit
AAACATCACCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACA335120.1180669489487983No Hit
CCGAAAGGTTGGTGGTTCGAGCCCACCCAGGGACGAGATCGGAAGAGCAC317440.11183806479561509No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA314340.11074589619409542No Hit
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGAGAGATCGGAAGAGCACAC297570.10483761637232605No Hit
AATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAGCA284290.1001589070083966No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)