FastQCFastQC Report
Wed 27 Sept 2023
ERR3403490_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3403490_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30037288
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA7738132.57617465331757No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGACGTAGATCGGAAGAGCAC5265561.753007794844861No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC2545770.8475365685477332No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG2278470.7585471764295099No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA2115390.7042546584099071No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT2110990.7027898124491132No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAA2065610.6876819238807446No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1865040.6209082524361054Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT1517670.5052619930268005No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1472910.49036051457108915No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA1466710.488296413444516No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC1313900.43742297906522054No Hit
GTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGAGATCGGAAGAGCACAC1256720.4183866399656321No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGCGTAGATCGGAAGAGCAC1125180.3745944041286284No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1094450.3643637867706299TruSeq Adapter, Index 4 (100% over 49bp)
AAAACATCACCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCAC920660.3065057005146403No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGT899860.2995809741545242No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT878620.2925097631983287No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGC820840.27327367237681377No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAG810960.2699844273557586No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG796530.26518039844342806No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGAGATCGGAAGAGCAC732490.2438602313231474No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGTAGATCGGAAGAGCA715820.2383104626489582No Hit
GCTTAACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGC711570.236895554618646No Hit
CTTAACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCA708890.23600333026070797No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAG703930.2343520493594495No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCAGATCGGAAG697320.23215145122289335No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT650250.2164809286377652No Hit
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGT637650.21228614247731017No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC635080.211430539268392Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGACCTCATAACCCAATTCAGACTACTCTCCTCCGCCAGATCGGAAGA631550.21025533330439156No Hit
ACGATCAAAAGGAACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACAC628420.20921329515500867No Hit
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCCAGATCGGAAGAGCACACG601780.20034431870147532No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG600970.20007465387687465No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG577150.1921445105163955No Hit
CGATCAAAAGGAACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACACG569970.18975414824400924No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC567950.18908165078019026No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT567080.18879201078339697Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA560390.1865647790839173No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGT555550.18495344852704412No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA530810.17671701919294444No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG519090.17281520222464825No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT512380.1705813121344377No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC508630.16933286387239754No Hit
AGTCGGCACTGGCAATTTTTGACAGTCTCTACGGAGACTGAGATCGGAAG505300.16822424181570586No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGCGAGATCGGAAGAGCACA493610.164332412433506No Hit
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGAGCAGATCGGAAGAGCACA484780.16139273292582204No Hit
AAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAGC475890.1584330782459455No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAG475580.15832987318961686No Hit
TGCTGAAGGCCTGTATCCTAGGCTACACACTGAGGACAGATCGGAAGAGC470680.15669856746055102No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG462620.15401523599600603No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC457620.15235063831328582No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC453740.15105891051149492No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG432800.1440875754162626No Hit
TCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCAC423130.14086824349788168No Hit
TCCTTAGGTCGCTGGTTCGATTCCGGCTCGAAGGAGATCGGAAGAGCACA416250.13857775708645867No Hit
GGGGACCTCATAACCCAATTCAGACTACTCTCCTCCGCCCAGATCGGAAG409450.13631390423795917No Hit
ATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAGCAC406080.13519196539980574No Hit
TCCTTAGGTCGCTGGTTCGAATCCGGCTCGAAGGAGATCGGAAGAGCACA404660.1347192196579132No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGA397940.1324820003723372No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG397340.13228224865041077No Hit
CGACTGCATAATTTGTGGTAGTGGGGGACTGCGTAGATCGGAAGAGCACA396930.13214575164042772No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACAC389970.1298286316660812No Hit
AAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGATCGGAAGAGCAC385520.12834713972846018No Hit
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCAGATCGGAAGAGCACAC384310.12794430708924187No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAGATCGGAAGAGC382400.12730843077444273No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACA379510.12634629331383046No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGCAGATCGGAAGAGCACAC371290.12360969472343841No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA356560.11870578995014462No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG356100.11855264696333438No Hit
AAACATCACCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACA355690.11841614995335131No Hit
CCGAAAGGTTGGTGGTTCGAGCCCACCCAGGGACGAGATCGGAAGAGCAC336000.11186096427879907No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA330280.10995666452976714No Hit
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGAGAGATCGGAAGAGCACAC313860.10449012573971392No Hit
CACGATCAAAAGGAACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACA303180.10093454508942351No Hit
AATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAGCA302190.1006049547482449No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)