FastQCFastQC Report
Wed 27 Sept 2023
ERR3403491_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3403491_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19266628
Total Bases982.5 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG658200.34162698319602164TruSeq Adapter, Index 4 (100% over 49bp)
AAGACTATACTTTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTT415420.21561634967987134No Hit
ATACTTACCTGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAG212710.11040333575755965No Hit
ATACTTACCTGGCAGGGGAGATACCATGATCACGAAGAGATCGGAAGAGC205740.10678568143839182No Hit
ATACTTACCTGGCAGGGGAGATACCATGATCACGAAAGATCGGAAGAGCA194530.10096733066107884No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)