FastQCFastQC Report
Wed 27 Sept 2023
ERR3403499_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3403499_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20040947
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG4440792.215858362381778TruSeq Adapter, Index 11 (100% over 49bp)
AAGACTATACTTTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTT241150.12032864514835552No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)