FastQCFastQC Report
Sat 24 Jun 2023
ERR3771179.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3771179.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176243483
Total Bases17.8 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT63880653.6245680641706337No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT61500833.4895378230808114No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC58205093.3025385681920505No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA57605823.268536176171689Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG38972442.21128403369105Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG34081751.9337878155755692No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA33027971.87399666857469Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA24120641.368597555462519No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC23904571.3563378113674707No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC21125481.1986531155878257No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG18954551.0754752276428854No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGAGATCGGAAGAGCACACGT18887481.0716696968590889No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC18676731.059711807897033No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT15606330.8854982739985908Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA15387140.8730615020811862Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT12314730.6987339214125722No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGT11849430.6723329452130721No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA11155590.6329646810259646Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACAC10490280.5952152000990584No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGAGATCGGAAGAGCACACG10058230.5707008184807605No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC8767900.49748789860218545No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCAC8357700.474213279137249No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC8083070.4586308589918187No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA7746340.4395249043052559No Hit
GTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAA7491860.42508578884587744Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGC6768450.3840397321244497No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCAC6495710.368564549986793No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG6454630.3662336836591002Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTC6446340.3657633116567493No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA6259430.3551580968244936No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG6259210.35514561409343004Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACA5552450.3150442731547696No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA4843050.2747931394433461No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC4710700.2672836419148616No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA4710510.2672728613743976No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC4573850.25951881579644No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG4460760.2531021246328864No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACG4183030.2373438114588328No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCA4124780.23403872471131315No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG4086240.23185197718771822Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCAGATCGGAAGAGCACACGT3987250.22623531560596769No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG3912660.22200310237854298No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGC3870920.21963478785765939No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT3781280.2145486423461116No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC3723950.21129575611031243No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGAGATCGGAAGAGCACACGTC3647060.20693304160358653No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAAGATCGGAAGAGCACACG3499080.19853670277272037No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCA3434150.19485259491836077No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGA3425430.19435782485074923Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC3351220.19014717270425258No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTCT3251680.18449930429484307Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGAGATCGGAAGAGC3212470.1822745411811908No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCAGATCGGAAGAGCACACG3204170.1818036017819734No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGAGATCGGAAGAGCA3150180.17874022609959428No Hit
GACGTACGCAGTTTTATCCGGTAAAGAGATCGGAAGAGCACACGTCTGAA2883140.16358846017585796Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACA2830100.16057898719579888No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTG2782700.15788952604846104Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT2746520.15583668418536645No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAAGATCGGAAGAGCACACGTC2571040.14588000397155113No Hit
GCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACAC2557150.14509188972394513No Hit
GCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTG2451240.13908258951055796Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGA2353100.13351415666246225Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACAC2291160.12999970047119416No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT2279010.12931031327836387No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGAGATCGGAAGAGCACACGTC2266460.12859823021087255No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC2242370.12723137115940905No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2235900.1268642653867661No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT2226370.1263235361729659Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC2217110.12579812667456192No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACA2209360.1253583941030035No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGAGATCGGAAGAGCACACGTCT2197540.12468773100676864Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTACGCAGTTTTATCCGGTAAAGAGATCGGAAGAGCACACGTCTGAAC2176170.12347520390300049Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC2087250.11842991096584264Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGGAGATCGGAAGAGCACACGTCT2049350.11627947684170542Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC2021500.11469927656842778No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGAGATCGGAAGAGCACAC1903560.10800739792460866No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGAGATCGGAAGAGCACACG1852540.10511253911158804No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)