FastQCFastQC Report
Mon 26 Jun 2023
ERR3771184.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3771184.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104517252
Total Bases6.2 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG34676323.317760401890398Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT28135912.6919871563404674No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA20716801.982141665951952Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA18206231.7419353888102607Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC16850851.6122553623970137No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT16130641.5433471212963004No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA10696161.02338702896628Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG9601960.9186961785026648No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT7437020.7115590830880245No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCAC7283410.6968619879137274No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC7264300.6950335816330111Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG7067910.6762433822886963No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGT7010650.6707648609054513No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAAGATCGGAAGAGCACACGTC6993710.6691440758507505No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG6063450.580138674139653Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGATCGGAAGAGCACAC5392380.515932049189353No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA5307130.5077755010244625Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG4858570.4648581843694092Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG4792880.4585730975781873Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA4441320.42493654540400655Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC4416730.42258382376911324No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACAC4341270.41536396307090045No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCAC4286390.41011315529038206No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT4034130.3859774269610533No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACG3882690.3714879530127715No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT3875140.3707655842310129No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCA3830150.3664610317156061No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACACGTC3777540.36142741295953706No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG3612320.34561949638706535No Hit
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTG3567020.34128528369651356Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC3551880.3398367190136227No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAGATCGGAAGAGCACACGTCT3517930.33658845144531735Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA3508090.3356469800794227Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA3445510.3296594518194948No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA3356370.32113071629552603Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCT3300860.3158196313848742Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC2972800.2844315118426573Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA2966860.2838631846156843No Hit
AGGGCTTCTGTCGTGAGTGGCACACGCAGAGATCGGAAGAGCACACGTCT2752760.2633785281687276Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCAC2740200.2621768126854311No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCACA2695670.25791627204282025No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG2554330.2443931457363613Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACA2522450.24134293159563744No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC2496420.23885243366329612No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2462010.2355601542221948Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA2401900.22980895058358403No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGCA2326140.2225603864900696No Hit
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC2324270.22238146865935587No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGAGATCGGAAGAGCACA2310560.2210697234940697No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG2302960.22034257081309408No Hit
GCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGAGATCGGAAGAGCACAC2195220.21003422478042189No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAGATCGGAAGAGCACACGTCTG2130420.2038342913952617Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT2084600.1994503261528537No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGATCGGAAGAGCACACGTCT2061130.19720476386041993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA2042190.19539262283704129No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGC2000010.19135692545762684No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG1984660.18988826839802486No Hit
GTGGAGGGGGGGGCGGCCCGCCGGCGGGGACAGATCGGAAGAGCACACGT1984030.18982799126789135No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAAGATCGGAAGAGCACACGT1923030.18399163422321896No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGAGATCGGAAGAGCAC1881710.18003821991033594No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1865600.1784968475826364No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1758150.1682162481654225No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCAGATCGGAAGAGCA1702810.16292142851210822No Hit
ATGATTAAGAGGGACGGCCGGGGGCATTCGTAGATCGGAAGAGCACACGT1702640.1629051632547706No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACGGGCAGATCGGAAGAGCACAC1697670.16242964367260632No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACA1616650.1546778133814693No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCA1582850.15144389751081477No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1520710.14549846756399604No Hit
ACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGAACTC1501950.14370354857779843Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTCT1489270.14249035173638128Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGATCGGAAGAGCACACGTC1421530.13600912507726476No Hit
AGACCCCAGAAAAGGTGTTGGTTGATATAGACAGATCGGAAGAGCACACG1418390.13570869620644063No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA1411140.13501503081998367No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCT1411060.13500737658123657Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC1402190.1341587128601506No Hit
GTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGC1392390.13322106861362945No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACAC1390890.13307755163712112No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC1386040.13261351341307748No Hit
AGCTAGCTGCGAGAATTAATGTGAATTGCAGATCGGAAGAGCACACGTCT1384340.1324508608397014Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAAGATCGGAAGAGCACACG1374600.13151895727224058No Hit
GTCGCGGCGTGGGAAATGTGGCGTAGATCGGAAGAGCACACGTCTGAACT1362950.13040430875469247Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCAGATCGGAAGAGCACACG1343870.1285787728135064No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC1342560.12845343465402248No Hit
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGT1334700.1277014056971188No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCAGATCGGAAGAGCACACGT1316290.1259399740054398No Hit
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTC1279600.12242954876004586No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGATCGGAAGAGCACACG1242340.11886458706357875No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGA1225500.11725336980731181Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACA1210400.11580863224379455No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCT1200260.11483845748259819Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGCAC1199050.11472268712154812No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT1197110.11453707183193068No Hit
AGTGAAAAAACGATCAGAGTAGTGGTATTTCAGATCGGAAGAGCACACGT1193550.1141964582076842No Hit
GCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGCACA1172130.11214703578314517No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCCAGATCGGAAGAGC1137340.10881839870799512No Hit
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC1116810.10685412968951767Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG1113350.1065230838637051No Hit
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGAGATCGGAAGAGCACACG1101270.1053672938128913No Hit
CGGTCACGCGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACA1076060.10295525182770783No Hit
GGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCACACGTCT1052880.10073743615073232Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)