FastQCFastQC Report
Sat 24 Jun 2023
ERR3771187_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3771187_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224389341
Total Bases22.6 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG81181633.617891546818171Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA31968061.424669276068688Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG29258201.3039032901299887Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT25115161.1192670689290898No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA24251571.0807808379810697Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACA23709721.0566330777717288No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG20043930.8932656921524628No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCT19732350.8793800058443952Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGT19359430.8627606781019068No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC17706950.7891172513403834Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGT17292740.7706578183675846No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA16851360.7509875435660734Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACATATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGT14015880.624623252492194No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT13240070.5900489720677061Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACAC13155320.5862720546962166No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT12480740.5562091293810609No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTC12166780.5422173774288146No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT11844640.5278610805314501Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA11334280.505116684664625Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCAC11235390.500709612583603No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACA10743290.4787789808607709No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA10497430.4678221324247305No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA10247380.4566785549764594Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGA8818030.3929790051836732Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA8674200.38656916417433573Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCT8408160.37471298603261194Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTC8251350.36772468617393017No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG8185800.36480342441934444Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC7595530.33849780770112425Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAAGATCGGAAGAGCACACG7376280.32872684447163647No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA7258140.32346188850387503Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA7186710.32027858221661254No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC6843160.30496814017560664Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACAC6838280.3047506610396436No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACG6540350.2914732923967186No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG6437490.2868892956907432No Hit
ACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAAC6301540.28083063000751Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAA6054160.26980604216846465Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC5609040.24996909278324408No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAAGATCGGAAGAGCACACGTC5369330.23928632153699314No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT5365350.23910895125807247No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT4903870.21854291198261508Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTC4493730.20026486017444117No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC4440520.197893535415303Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT4408500.196466551412529Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAAC4289880.191180204054345Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACGTACGCAGTTTTATCCGGTAAAGAGATCGGAAGAGCACACGTCTGAA4127550.18394590320580334Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGCTTCTGTCGTGAGTGGCACACGCAGAGATCGGAAGAGCACACGTCT4120300.18362280407962872Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC4059940.1809328367339873Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACG4049080.18044885652567605No Hit
AAATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGT3961000.1765235363831297No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT3902100.17389863451669035No Hit
ATGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAA3767090.16788186030636812Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG3736280.16650880043361774No Hit
CACATATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACG3724630.16598961356190264No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC3658340.16303537341374874Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG3600180.16044344994087756TruSeq Adapter, Index 4 (100% over 49bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC3531800.1573960681135919No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG3480610.15511476545581548Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTACGCAGTTTTATCCGGTAAAGAGATCGGAAGAGCACACGTCTGAAC3385270.15086590053312737Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC3383020.15076562839052143Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC3306210.14734256026893897No Hit
CTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAACT3239210.1443566786891183Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA3088570.1376433473281603No Hit
TAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACT3086910.13756936876961548Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC2931890.1306608409710513No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCT2921430.13019468692142558Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC2872210.1280011780951752No Hit
AATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTC2857930.12736478423010297No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT2833020.12625466019796366Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG2812720.12534998264467473No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAGATCGGAAGAGCACACGTCT2700160.120333701590576Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG2697510.12021560328928457Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTACGCAGTTTTATCCGGTAAAGCGAAGATCGGAAGAGCACACGTCTG2657470.11843120480486638Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACTCCA2653770.11826631283702554Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCT2649730.11808626863430202Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTG2633740.11737366794084929Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAGATCGGAAGAGCACACGT2613100.11645383815267767No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGATCGGAAGAGCACACGTCT2604330.11606299962349814Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT2595970.11569043290697127No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC2529090.11270989917475625No Hit
GCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGATCGGAAGAGCACACGT2516110.11213144032541189No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTCT2499000.11136892638763977Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTTAAGGAGAGGGTATCAAGCACATTAAAGATCGGAAGAGCACACGTCT2455290.10942097289728214Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT2431760.10837234911260779No Hit
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC2375950.1058851543220139Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA2364420.10537131529790446Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2315780.10320365440174807No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCAGATCGGAAGAGCACACGTCTG2251060.10031938192643472Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)