FastQCFastQC Report
Thu 6 Jul 2023
ERR3771191_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3771191_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294527983
Total Bases15 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC136517804.635138522644213No Hit
CACGAGCGTACGCGTTAGGACCCGAAAAGATCGGAAGAGCACACGTCTGA68081242.3115372368539937Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG62852692.1340142067247987No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC35304211.1986708237498778No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACACGTCTG35083141.1911649155591442Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACGAGCGTACGCGTTAGGACCCGAAAAGATCGGAAGAGCACACGTCTG34075471.156951867626106Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG28845740.9793887733920346No Hit
AGCACGAGCGTACGCGTTAGGACCCGAAAAGATCGGAAGAGCACACGTCT25070700.8512162323129753Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG23714300.8051628832836573TruSeq Adapter, Index 4 (100% over 49bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG22034620.7481333276234061No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT20019370.6797102874941428No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT18038450.612452841195738No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT15939690.5411944168306752No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA14491390.49202082098935906Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTGGTGATAGCTGGTTACCCAAAAAAAGATCGGAAGAGCACACGTCT14264280.4843098389058672Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG13992970.47509815052106613Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT12908970.4382934982446133Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCACGAGCGTACGCGTTAGGACCCGAAAAGATCGGAAGAGCACACGTC11174680.3794097893917265No Hit
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA9903840.3362614274922733Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTTGGTGATAGCTGGTTACCCAAAAAATAGATCGGAAGAGCACACGTC9434250.32031761138295645No Hit
AGCGGAGGAAAAGAAACTAACCAGGATTCCCAGATCGGAAGAGCACACGT9122990.3097495153796643No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA8888680.30179407435116273Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACGT8105520.27520373166036316No Hit
CGCCGGGTTAAGGCGCCCGATGCCGACGCTCAGATCGGAAGAGCACACGT7693670.2612203404794987No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG7421660.25198488525282164No Hit
AGCGGAGGAAAAGAAACTAACCAGATCGGAAGAGCACACGTCTGAACTCC6903290.2343848597910644Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAGCACGAGCGTACGCGTTAGGACCCGAAAAGATCGGAAGAGCACACGT6856180.23278535133281378No Hit
GCCGGGCGTGGAATGCGAGTGCCTAGTGGGAGATCGGAAGAGCACACGTC6322660.21467094350759874No Hit
CACGAGCGTACGCGTTAGGACCCGAAAGATGGAGATCGGAAGAGCACACG6216020.21105023491095584No Hit
TGGAGCACGAGCGTACGCGTTAGGACCCGAAAAGATCGGAAGAGCACACG5750860.19525682895808238No Hit
AACGCGCCCGATCTCGTCTGATCTCGGAAGCAGATCGGAAGAGCACACGT5563110.1888822224406433No Hit
AGTCAGAATCCCGCCCAGGCGGAACGATAAGATCGGAAGAGCACACGTCT5468220.1856604572612036Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGGAGGAAAAGAAACTAACCAGGATTCCCTAGATCGGAAGAGCACACG5331580.18102116972702045No Hit
AAGTCTTCCCCAGGCCAAGTCAAGGAAGAGATCGGAAGAGCACACGTCTG5107930.17342766374765822Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA5107560.17341510127409523Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAAGCTATCCAAGAGAAGATCAAAGAGAAGATCGGAAGAGCACACGTCTG4791280.16267656306192135Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGAGCGTACGCGTTAGGACCCGAAAAGATCGGAAGAGCACACGTCTGAA4779900.16229018211828108Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCGGGCGTGGAATGCGAGTGCCTAGTGGGAGATCGGAAGAGCACACGT4733950.16073005871228202No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGAGATCGGAAGAGCACACGTC4642920.15763935068947252No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT4570750.1551889892920633No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGT4489650.1524354308975796No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC4436110.15061760702038285No Hit
GATCTGAAGCTGGACTATCTGGACCTGTAGATCGGAAGAGCACACGTCTG4247420.14421108502956748Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGCGTGGAATGCGAGTGCCTAGTGGGAGATCGGAAGAGCACACGTCTG4214440.14309132725089826Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGACCGGCTCCGGGACGGCCGGGAAGGCCAGATCGGAAGAGCACA4211480.14299082746239428No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGAGATCGGAAGAGCACACGT4199040.14256845672962762No Hit
CCGGGCGTGGAATGCGAGTGCCTAGTGGGAGATCGGAAGAGCACACGTCT4176590.14180622015803504Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC4089130.13883672302879282No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACAC4073370.1383016295602717No Hit
CGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACGTC3997710.1357327734797953No Hit
TCCATCTAAGGCTAAATACCGGCACGAGAGATCGGAAGAGCACACGTCTG3964270.13459739749075048Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACGAGCGTACGCGTTAGGACCCGAAAGAAGATCGGAAGAGCACACGTCT3962700.13454409185968588Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTGGTGATAGCTGGTTACCCAAAAAAAGATCGGAAGAGCACACGTCTG3956750.1343420737037404Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGAGATCGGAAGAGCACACG3912560.13284170692874367No Hit
GGAGCACGAGCGTACGCGTTAGGACCCGAAGATCGGAAGAGCACACGTCT3887330.13198508204227238Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC3865410.13124084036524297No Hit
TGGGAGGCCATGGAGGAACTGGTGGACCAGATCGGAAGAGCACACGTCTG3816150.12956833374980198Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGCGGAGGAAAAGAAACTAACCAGATCGGAAGAGCACACGTCTGAACTC3808740.12931674475222954Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGCCACAGGGATTTCAGGCTGGGAATGAGATCGGAAGAGCACACGTCTG3768100.12793690981817507Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAA3692050.12535481221151062Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTCAGATCGGAAGAGCACACG3599350.12220740329451142No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATAAGATCGGAAGAGCACACGTC3544890.12035834299656341No Hit
GCCTTTTACTAAAGATTTCCGTGGAGAGGAGATCGGAAGAGCACACGTCT3414250.11592277124988834Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG3409590.1157645519882571Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG3409230.11575232904100662Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC3168610.10758264690930912No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT3156900.10718506159735593Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGT3134580.10642723886782601No Hit
GAACGCGCCCGATCTCGTCTGATCTCGGAAGCAGATCGGAAGAGCACACG3098820.1052130927742781No Hit
CGGGTGATGCGAATCGTAATCTGAGCCGAGATCGGAAGAGCACACGTCTG2981970.10124572781255899Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG2981630.10123418391793354Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)