FastQCFastQC Report
Fri 30 Jun 2023
ERR3771192.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3771192.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223927338
Total Bases12.5 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC2659997011.87883991190035No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG51110702.282468074532284No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGT47158332.105965730722883No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG33990171.517910689404078No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGAGATCGGAAGAGCACACGT30820291.3763522701279107No Hit
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA28721221.2826133805958075Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA24858901.110132430547627Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG23004281.0273100285772165Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGCCGCGACCGGCTCCGGGACGGCCGGAGATCGGAAGAGCACACGTC19902510.8887932209509855No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA19379930.8654561865063568No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACG18100960.8083407841877708No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA16763590.7486173930223741Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT16571530.7400405036744553No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC15435840.6893236054992089No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC15275710.6821726251218152No Hit
CAAAAAGTAAGTTTAGATTATAGCCAAAAGATCGGAAGAGCACACGTCTG14811670.6614498315520546Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT13443890.6003684105778992No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA12919780.5769630503980715Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGAGATCGGAAGAGCACACG12333330.5507737514389601No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT11136650.4973332018978406No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACACGTC10187920.4549654406198496No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA10139720.4528129566743655No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGT8624050.3851271612044082No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTCT7967720.3558172070977774Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAAAAAGTAAGTTTAGATTATAGCCAAAAGAGAGATCGGAAGAGCACACG7522800.33594826193128774No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT6360180.28402874150185276No Hit
AATGCCTAAAGGAAAGATCCAAAAAGAGATCGGAAGAGCACACGTCTGAA6246130.2789355715022165Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAAAAAGTAAGTTTAGATTATAGCCAAAAAGATCGGAAGAGCACACGTCT6068100.2709852246803381Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT5956750.2660126295075236No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG5872790.26226319896680056No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT5594350.24982880830745194Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCACAC5558370.24822203709669427No Hit
GTGGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGC5527000.24682113623839894No Hit
AATGCCTAAAGGAAAGATCCAAAAAGAAGATCGGAAGAGCACACGTCTGA5310570.23715594743505594Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC5096700.22760508143047725No Hit
ACGTACGCAGTTTTATCCGGTAAAGAGATCGGAAGAGCACACGTCTGAAC4828840.21564316546289672Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC4790600.21393546865635496No Hit
GACGTACGCAGTTTTATCCGGTAAAGAGATCGGAAGAGCACACGTCTGAA4673670.20871368550810887Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC4600770.2054581651839223No Hit
GTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGAA4475510.19986438636625958Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAAAAAGTAAGTTTAGATTATAGCCAAAAGAGATCGGAAGAGCACACGTC4339550.19379277397563668No Hit
GTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAAC4266580.1905341276374214Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGAGATCGGAAGAGC4235680.18915421573046162No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT4016660.17937336440805632Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTTGGTGATAGCTGGTTACCCAAAAAAAGATCGGAAGAGCACACGTCT3963620.17700473892115842Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA3885520.17351700041198184No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC3808470.17007615211323596No Hit
TTAATCAGTGAAATTGACCTTTCAGTGAGATCGGAAGAGCACACGTCTGA3780790.16884003685159693Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCAGATCGGAAGAGCAC3671060.16393978657487546No Hit
CCGACGTACGCAGTTTTATCCGGTAAAAGATCGGAAGAGCACACGTCTGA3641240.1626081045986444Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAAAAAGTAAGTTTAGATTATAGCCAAAGATCGGAAGAGCACACGTCTGA3622790.1617841766153626Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGACGTACGCAGTTTTATCCGGTAAAGATCGGAAGAGCACACGTCTGAA3542140.15818256188085442Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT3487050.15572238884025855Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGGCGTGGGAAATGTGGCGTACGGAAGAAGATCGGAAGAGCACACGTC3358910.14999999687398596No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC3344520.14935737770436944No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATGAGATCGGAAGAGCACACGT3231700.14431913623695203No Hit
AGCTTGGTGATAGCTGGTTACCCAAAAAATAGATCGGAAGAGCACACGTC3195110.14268512404680128No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCCGGGAGATCGGAAGAGCAC3177260.14188799046947986No Hit
TGGGAAATGTGGCGTCCCGGAGTCGGGTAGATCGGAAGAGCACACGTCTG3047700.13610218507576774Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTG3003160.13411314700664195Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCAGATCGGAAGAGCACACGT2997860.13387646308732523No Hit
CAGTAAGTGCGGCTACGCCGGCAGGCGCAGATCGGAAGAGCACACGTCTG2944320.13148550892879368Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTG2926910.1307080245825099Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCT2901470.12957194176978964Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTC2793160.12473510492050774No Hit
TAATCCACCTATAACTTCTCTGTTAACCCAACAGATCGGAAGAGCACACG2770810.12373701329848345No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTGA2741620.12243346544851079Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCAGATCGGAAGAGCACACG2706200.12085170234998283No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC2520030.11253784475390852No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC2512260.11219085719672156No Hit
AATGCCTAAAGGAAAGATCCAAAAAGATAGATCGGAAGAGCACACGTCTG2502450.11175276865926928Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAAGTAAGTTTAGATTATAGCCAAAAGATCGGAAGAGCACACGTCTGA2492250.11129726375794277Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACACG2424670.10827932049993824No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGAAGATCGGAAGAGCACACG2370130.10584370899813939No Hit
CAAAAAGTAAGTTTAGATTATAGCCAAAAGAAGATCGGAAGAGCACACGT2328910.10400293330866105No Hit
CGTCGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACG2310490.10318034504567727No Hit
TAGCTGCGAGAATTAATGTGAATTGCAGATCGGAAGAGCACACGTCTGAA2302080.10280477678879923Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)