FastQCFastQC Report
Fri 23 Jun 2023
ERR3804289_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3804289_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4668320
Total Bases685.5 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTACTTCT2334345.00038557768105No Hit
CTTCTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTACT2122594.5467962778901185No Hit
CCGCTTTATTGATAATAGAATCGGAAGTAACTATGCCAATCCTTTTGTAA1696193.633405593446893No Hit
CTTTCTTATTTCTTACTCTTATTTACTAACAAATTTACTCTTTTACAACA979882.0989992117078518No Hit
CTCTTTTACAACAAGCTTACGATCTTGAAGCTCAGTCATCCCCAAAATGA884131.893893306371457No Hit
CTCTTATTTACTAACAAATTTACTCTTTTACAACAAGCTTACGATCTTGA645561.3828529321040546No Hit
TTCTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTACTT627381.3439095863179902No Hit
CGCTTTATTGATAATAGAATCGGAAGTAACTATGCCAATCCTTTTGTAAC511991.0967328717825684No Hit
CTTATTTCTTACTCTTATTTACTAACAAATTTACTCTTTTACAACAAGCT453280.9709702848133803No Hit
TTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTACTTCTT451060.9662148267470954No Hit
CTGGTCTATTATCTTTTAAAATCCTTCTCAGCAGATTTTTGAAACACTTC444730.9526553449634986No Hit
CCCGCTTTATTGATAATAGAATCGGAAGTAACTATGCCAATCCTTTTGTA420920.9016519861534771No Hit
CTTCTCAGCAGATTTTTGAAACACTTCTTCAATCAATTCTTTCACTTAAT395600.8474140590190905No Hit
CTTTATTGATAATAGAATCGGAAGTAACTATGCCAATCCTTTTGTAACTT359780.7706841004901122No Hit
CTCTCTTCGACATACCGTCATAGGCGTTACTCAAGCAGTTAACTCAAGTT311620.6675206498269185No Hit
CTTTTACAACAAGCTTACGATCTTGAAGCTCAGTCATCCCCAAAATGAGT295310.6325830277273194No Hit
CTTATTTACTAACAAATTTACTCTTTTACAACAAGCTTACGATCTTGAAG239440.5129039997258114No Hit
GCTTTATTGATAATAGAATCGGAAGTAACTATGCCAATCCTTTTGTAACT237410.5085555403228571No Hit
CACCTCTCTTCGACATACCGTCATAGGCGTTACTCAAGCAGTTAACTCAA232100.4971809987318779No Hit
CTTCTTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTAC231310.4954887411317133No Hit
CTATTATCTTTTAAAATCCTTCTCAGCAGATTTTTGAAACACTTCTTCAA227060.4863848236624739No Hit
CTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTTATCCT221500.47447475751448054No Hit
GTGGATCATTATTTGTCATTATTCGGTTATCAACGCCTCTCTACTTTAGT205160.43947287246803984No Hit
CTTTTTTATATTTATTACAAAAGTTATTATAAGTCTAAGTATGAATCAGC203880.4367309867361278No Hit
CTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTTATCCTTACACCTA202430.43362494430544607No Hit
CTTTACTTCTTTACTTCTTTTATCCTTACACCTAACTCTTTACACCTTGA197660.42340713575761735No Hit
CTTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTACTTC197610.4233000308462144No Hit
CTTTACTTCTTTACTTCTTTACTTCTTTTATCCTTACACCTAACTCTTTA196480.4208794598485108No Hit
CTTTACTTCTTTTATCCTTACACCTAACTCTTTACACCTTGACTATATCA191080.40931212941700656No Hit
CTACTTCTTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTACTTCTT165970.3555240429105117No Hit
CTATAAGCTCCATTTGTAGTACCGCTTCTTCAGAAACCATTGGTTTTAGT157720.3378517325290468No Hit
CTTCTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTTAT151460.32444219762141413No Hit
GTCCGTGTCGGCGTTGGCGAATACAAAGAAATTATGCCCTATAAGCTCCA147720.3164307502484834No Hit
CTTCAATCAATTCTTTCACTTAATTCCTTTCTTATTTCTTACTCTTATTT146370.3135389176406074No Hit
CTTCTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTTATCCTTACAC137180.2938530349247695No Hit
TTTTACAACAAGCTTACGATCTTGAAGCTCAGTCATCCCCAAAATGAGTT134070.28719110943551424No Hit
CCCAAAATGAGTTTCTGGTAGATGCTTACAAATCTTTTCTTAAAAATCTT132900.28468485450868836No Hit
GTCTATTATCTTTTAAAATCCTTCTCAGCAGATTTTTGAAACACTTCTTC131770.2822642835109847No Hit
CTTCGACATACCGTCATAGGCGTTACTCAAGCAGTTAACTCAAGTTAGGC130890.2803792370702951No Hit
CTCAGCAGATTTTTGAAACACTTCTTCAATCAATTCTTTCACTTAATTCC129520.27744456249785787No Hit
CTTCTTTACTTCTTTACTTCTTTACTTCTTTTATCCTTACACCTAACTCT127960.27410288926209No Hit
ATCATTATTTGTCATTATTCGGTTATCAACGCCTCTCTACTTTAGTTTTT127930.2740386263152483No Hit
TTTCTTATTTCTTACTCTTATTTACTAACAAATTTACTCTTTTACAACAA127200.27247489460876717No Hit
CTTCTTTACTTCTTTACTTCTTTTATCCTTACACCTAACTCTTTACACCT115910.24829060561401103No Hit
CTTCTTTACTTCTTTTATCCTTACACCTAACTCTTTACACCTTGACTATA115070.24649124310244372No Hit
CTTAATTCCTTTCTTATTTCTTACTCTTATTTACTAACAAATTTACTCTT110170.2359949617849676No Hit
ATTATCTTTTAAAATCCTTCTCAGCAGATTTTTGAAACACTTCTTCAATC107870.23106813586043803No Hit
CTCAATAATCTAATAGCTACTTCTTTTACTTCTTTACTTCTTTACTTCTT104030.22284247866470164No Hit
CCACCATGCCGTGATCCGAGTGTTTTGAAACCTGCGCTAGCAGGTGGGTC102650.21988638310998387No Hit
CTTCTACTTACAATTCCTACCAATTGATGGTCCTTCACCACAGGAACTCT101850.2181727045275388No Hit
CAATAATCTAATAGCTACTTCTTTTACTTCTTTACTTCTTTACTTCTTTA101720.21789423175789152No Hit
ACCCGCTTTATTGATAATAGAATCGGAAGTAACTATGCCAATCCTTTTGT100000.21420982280563458No Hit
GCTACTTCTTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTACTTCT91740.19651609144188914No Hit
ACTTCTTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTA90260.19334578606436575No Hit
TTCTTATTTCTTACTCTTATTTACTAACAAATTTACTCTTTTACAACAAG84070.180086198032697No Hit
CCCCGCTTTATTGATAATAGAATCGGAAGTAACTATGCCAATCCTTTTGT83160.1781368886451657No Hit
CTGCTTTATTGATAATAGAATCGGAAGTAACTATGCCAATCCTTTTGTAA79800.17093943859889638No Hit
TCTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTACTTC78530.16821897384926482No Hit
CTTACACCTAACTCTTTACACCTTGACTATATCATACTTCTTGCATTACT77390.16577698186928058No Hit
ATTATTTGTCATTATTCGGTTATCAACGCCTCTCTACTTTAGTTTTTTAA76350.163549199712102No Hit
CATTATTTGTCATTATTCGGTTATCAACGCCTCTCTACTTTAGTTTTTTA72090.15442386126058197No Hit
GCCCGCTTTATTGATAATAGAATCGGAAGTAACTATGCCAATCCTTTTGT68150.14598399424203995No Hit
CTTTTAAAATCCTTCTCAGCAGATTTTTGAAACACTTCTTCAATCAATTC63890.13685865579051992No Hit
GGATCATTATTTGTCATTATTCGGTTATCAACGCCTCTCTACTTTAGTTT62980.13490934640298866No Hit
TTATTTACTAACAAATTTACTCTTTTACAACAAGCTTACGATCTTGAAGC61050.13077509682283991No Hit
CCGCATTTGGTCTATCTCTATAGTTTTCAATTAAAGCAGTACCACCAACG60920.1304966240531926No Hit
CTTTCACTTAATTCCTTTCTTATTTCTTACTCTTATTTACTAACAAATTT59850.1282045789491723No Hit
GGCGGGGTTCATGACTTTCCGTTGCGTAGGACCCAACTATCATCATCACT59440.12732631867566918No Hit
CCTTTCTTATTTCTTACTCTTATTTACTAACAAATTTACTCTTTTACAAC57540.12325633204236214No Hit
CTCGTCCGTGTCGGCGTTGGCGAATACAAAGAAATTATGCCCTATAAGCT56480.1209857079206224No Hit
CCTCTCTTCGACATACCGTCATAGGCGTTACTCAAGCAGTTAACTCAAGT55850.11963618603694692No Hit
TTACTTCTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTTACTTCTTT55670.11925060835589678No Hit
TTATCTTTTAAAATCCTTCTCAGCAGATTTTTGAAACACTTCTTCAATCA54410.11655156458854578No Hit
CTGCCTTTTTCACCTTGTAATTCAGCTACAGCAATAAAAGCAGGATACAT54260.11623024985433733No Hit
GGTCTATTATCTTTTAAAATCCTTCTCAGCAGATTTTTGAAACACTTCTT51820.11100353017787984No Hit
CTCTTCGACATACCGTCATAGGCGTTACTCAAGCAGTTAACTCAAGTTAG49150.10528412790896939No Hit
CTCGCTTTATTGATAATAGAATCGGAAGTAACTATGCCAATCCTTTTGTA49110.10519844397984715No Hit
CTGTGCACCTCTCTTCGACATACCGTCATAGGCGTTACTCAAGCAGTTAA48510.10391318504301335No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTAATCTCGTATGC48230.10331339753915755TruSeq Adapter, Index 16 (97% over 36bp)
CACCCGCTTTATTGATAATAGAATCGGAAGTAACTATGCCAATCCTTTTG48080.1029920828049491No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)