FastQCFastQC Report
Sat 24 Jun 2023
ERR3804291_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3804291_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8592897
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACCTCTCTTCGACATACCGTCATAGGCGTTACTCAAGCAGTTAACTCA1455831.6942248929551933No Hit
CACCTCTCTTCGACATACCGTCATAGGCGTTACTCAAGCAGTTAACTCAA776410.9035485936815022No Hit
GTGCACCTCTCTTCGACATACCGTCATAGGCGTTACTCAAGCAGTTAACT730550.8501789326696223No Hit
GCCCGTTCTACAAAAAGTACGCGGTCACGCATATATAGCGCTTCCACAGC702960.818071018423705No Hit
CTCTCTTCGACATACCGTCATAGGCGTTACTCAAGCAGTTAACTCAAGTT575360.6695762791058708No Hit
GGCCCGTTCTACAAAAAGTACGCGGTCACGCATATATAGCGCTTCCACAG573280.667155675204765No Hit
CCCCGGTCTACCTTCTATTACCTATGAATTCAGTAATAGATACTTGGACA509210.5925940925394544No Hit
GTCCCTACTTGGTCTTGCTCCAGATGGGGTTTACATAGCCAACTAGTCAC443520.5161472318357825No Hit
GCCCGTTCTACAAAAAGTACGCGGTCACGCATTAAAAGCGCTTCCACAGC383700.4465315946414812No Hit
GTCGTCAACTGCAAAATTCACACCCGCCACTCACTCAAAGTGGGTGACAC374940.43633712821182424No Hit
GTCCCGTGGTACTCTGGATCATTCTCAAAGTTCTTCAGTGTTTCGCTTAC337020.39220765709166533No Hit
GGCCCGTTCTACAAAAAGTACGCGGTCACGCATTAAAAGCGCTTCCACAG317380.3693515702562244No Hit
CCTCTCTTCGACATACCGTCATAGGCGTTACTCAAGCAGTTAACTCAAGT304380.35422279587431343No Hit
GTCCCGTGGTACTCTGGATCATTCTCAAAGATTTTCAGTGTTTCGCTTAC303100.35273319347363297No Hit
GCCCCCACACCCGCCTGCTGGAAGGTTCGCCCAGGGGTTACCTTCACTAC272270.3168547231509932No Hit
CCCGGACATTTTCGGCGCAGAATCACTCGACCAGTGAGCTATTACGCACT248400.28907596588205353No Hit
CTCCGTCACTCTCATTTCAAGGCTAGCCCTAAAGCTATTTCGGCGAGAAC244910.2850144718364482No Hit
GGCGTTACTCAAGCAGTTAACTCAAGTTAGGCTACCCCACGGCACACGAA225830.2628100860513049No Hit
CTTCGACATACCGTCATAGGCGTTACTCAAGCAGTTAACTCAAGTTAGGC222140.25851584163059327No Hit
GCTCCCCATCACCTCTCAGGATTATGCTAAAACGGATTTGCCTGTCTTAG220790.2569447765986256No Hit
GTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGGAGGAGCA205440.23908118530921527No Hit
GTCCCGACTAACCCTGAGCGGACGAACCTTCCTCAGGAAACCTTAGGTTT201360.23433307765704628No Hit
CCCCCACACCCGCCTGCTGGAAGGTTCGCCCAGGGGTTACCTTCACTACT199120.23172627345585547No Hit
GTCACTAGGTAGTATTTAGCCTTGGGGGGTGGTCCCCCCTGCTTCCCACA198080.23051597150530256No Hit
GGGACCTTAGCTGATGATCTGGGTTCTTTCCCTCTTGACCACGGATCTTA193370.22503470017154864No Hit
GCTTCCTTCCGGACCTGGCGGGGTTCATGACTTTCCGTTGCGTAGGACCC190960.22223005815151745No Hit
GTCCGCAACCCCAAAAACTTAAGTTCTTGGTTTGGGCTCTTCCCCTTTCG186720.21729575019926342No Hit
GCCCCGGACATTTTCGGCGCAGAATCACTCGACCAGTGAGCTATTACGCA184410.2146074833667854No Hit
CCCCGGACATTTTCGGCGCAGAATCACTCGACCAGTGAGCTATTACGCAC179570.20897492428921235No Hit
GTCCAGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCGCT173820.20228335100490555No Hit
GGGCGCATGAGTACGTGGTCGTAGACCCGAAACCAGGTGATCTACCCATG169360.19709301764003453No Hit
CCCTACTTGGTCTTGCTCCAGATGGGGTTTACATAGCCAACTAGTCACCT166250.19347374930713124No Hit
CCTGAACATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGA163010.1897031932304088No Hit
GGCCCTTTACCTTCAGCTTCTACGAGCGTAGTTACTGTTTTAGATCTCGT161600.1880623030859092No Hit
GGGGTTTACATAGCCAACTAGTCACCTAGTTGCTGGTGAGCTCTTACCTC160810.18714293910423924No Hit
CTCCCCATCACCTCTCAGGATTATGCTAAAACGGATTTGCCTGTCTTAGC159660.1858046244473779No Hit
GGCCTGTTCTCTGCGACCGAAGTGTGCTTACAGCGTATAGCCTTCACACC155790.18130090468907054No Hit
GCCGAGTTCTGTATTGGATAATCATCTATCTAGGCCAATTGTTGCCAATT147280.17139737622829646No Hit
GGTCACTAGGTAGTATTTAGCCTTGGGGGGTGGTCCCCCCTGCTTCCCAC142620.16597429248831913No Hit
GGCGGGGTTCATGACTTTCCGTTGCGTAGGACCCAACTATCATCATCACT142130.16540405406930864No Hit
CCCCACACCCGCCTGCTGGAAGGTTCGCCCAGGGGTTACCTTCACTACTA140440.16343731339966022No Hit
GGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAAC139370.16219209889284136No Hit
CCCCCCTGCTTCCCACAGGGTTTCACGTGTCCCGTGGTACTCTGGATCAT135730.1579560420659063No Hit
GGGGTTCATGACTTTCCGTTGCGTAGGACCCAACTATCATCATCACTTAC132810.154557886589354No Hit
CTCCAGATGGGGTTTACATAGCCAACTAGTCACCTAGTTGCTGGTGAGCT131540.15307992170742882No Hit
CCCGAGATGAGTTCTCCCTGACTCCTTGAGAGTCCTGAAGGAACGTTGAA129980.1512644687815995No Hit
CTCTTCGACATACCGTCATAGGCGTTACTCAAGCAGTTAACTCAAGTTAG128600.14965849119336586No Hit
CCCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCACTCGGTACCA123800.14407248219081412No Hit
GGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTAC123290.143478968734293No Hit
GTCCAGCAACATACTATAGATTCGACCAGCCCTAATTATACAACTTAAGG122340.14237340445253796No Hit
GGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGGAGGAGCACG122020.14200100385236783No Hit
GGGGTCTTACTCACTTGCGTGATGGGAAATCTTATCTTGAGGGGGGCTTC120870.14066268919550648No Hit
GTTGCTTCGAATTAAACCACATGCTCCGCTGCTTGTGCGGGTCCCCGTCA120170.13984806288263432No Hit
CTGGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGGAGGA118820.13827699785066666No Hit
CTCCCACCTATCCTGTACATCTAGTACCAAAATCCAATGTCAAGCTGCAG110870.12902517044019032No Hit
CACCCTTACTAGACAGCTCAAAAGCTGTTCTCACCACAAGTATCAGTATT110480.12857130720873297No Hit
CCCACCTATCCTGTACATCTAGTACCAAAATCCAATGTCAAGCTGCAGTA109970.12797779375221185No Hit
GGCAGGTCTCCTGACTCGTGATTCTCTTTACTTACAGCACCTTCCCAGAA107790.12544081466355292No Hit
TGCACCTCTCTTCGACATACCGTCATAGGCGTTACTCAAGCAGTTAACTC106450.12388138715034057No Hit
GTTGCACTTTCCTTGGAGTCACCTCCACTGGGTATTACCCAGCACCCTGC105040.122240497005841No Hit
ATCGGTCACTAGGTAGTATTTAGCCTTGGGGGGTGGTCCCCCCTGCTTCC104860.12203102166824528No Hit
GCTCGCCTACCGAATATTTCTATTCCCATAGCTTCGGTGATACGTTTGAG102470.11924965468572474No Hit
CCGGACATTTTCGGCGCAGAATCACTCGACCAGTGAGCTATTACGCACTC101100.11765531461624641No Hit
CGGTCACTAGGTAGTATTTAGCCTTGGGGGGTGGTCCCCCCTGCTTCCCA100950.11748075183491667No Hit
GCCCCCTTTACATAACAGGCTGTTCCCAAACAAACTCCTATTACATAATC100180.11658466289075732No Hit
GTCCGCACTGTAATCCTTATTACGCTTCTAAATCGAACAGTCTAGGAGTT96950.1128257443327902No Hit
GCTCCAGATGGGGTTTACATAGCCAACTAGTCACCTAGTTGCTGGTGAGC96070.11180164268232237No Hit
CGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGG94800.11032367780039723No Hit
CCCAACTATCATCATCACTTACCTAAGCCAGCCCTACAACCAATATACCT93640.10897372562478057No Hit
GCTCCCTACTGGCTTAGACCCACTCAACCAACGGTGGGCTCAACCTATCC93480.10878752532469549No Hit
GCCCTGAACATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCC92390.10751903578036603No Hit
GCACTTTCCTTGGAGTCACCTCCACTGGGTATTACCCAGCACCCTGCCCT91540.10652984668616416No Hit
GTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGA89950.1046794812040689No Hit
GGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGGAGGAGCACGA89040.10362046699733513No Hit
CTCCTCTCCTGCACTCAAGCCAATAAGTTTCTAAGGCTTACTACGGTTGA88440.10292221587201615No Hit
CCCTGAACATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCG88370.10284075324072893No Hit
GCGCTATCGGTCACTAGGTAGTATTTAGCCTTGGGGGGTGGTCCCCCCTG87820.10220068970918654No Hit
CCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCCTC86120.10022231152078281No Hit
CTGGGTTCTTTCCCTCTTGACCACGGATCTTATCACTCGTAGTCTGACTC85940.10001283618318713No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)