FastQCFastQC Report
Fri 23 Jun 2023
ERR3804292_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3804292_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6693980
Total Bases816 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACCTCTCTTCGACATACCGTCATAGGCGTTACTCAAGCAGTTAACTCA1133641.6935216418334085No Hit
GCCCCGGACATTTTCGGCGCAGAATCACTCGACCAGTGAGCTATTACGCA1000741.4949850462654504No Hit
CCCGGACATTTTCGGCGCAGAATCACTCGACCAGTGAGCTATTACGCACT868701.297733187132319No Hit
CCCCGGACATTTTCGGCGCAGAATCACTCGACCAGTGAGCTATTACGCAC774151.156486873280171No Hit
GTGCACCTCTCTTCGACATACCGTCATAGGCGTTACTCAAGCAGTTAACT528770.7899187030735078No Hit
GTCCAGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCGCT527110.7874388629783775No Hit
CACCTCTCTTCGACATACCGTCATAGGCGTTACTCAAGCAGTTAACTCAA425510.6356606981198032No Hit
CTGGCAACTAAAGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACAT406110.6066794343574375No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC363600.5431746136080478TruSeq Adapter, Index 12 (100% over 50bp)
GCCCTGAACATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCC347940.5197804594576022No Hit
CTCTCTTCGACATACCGTCATAGGCGTTACTCAAGCAGTTAACTCAAGTT327010.4885135599449057No Hit
CCGGACATTTTCGGCGCAGAATCACTCGACCAGTGAGCTATTACGCACTC286810.42845960101464303No Hit
GTCCCTACTTGGTCTTGCTCCAGATGGGGTTTACATAGCCAACTAGTCAC276840.4135656216481077No Hit
CCCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCACTCGGTACCA273930.40921843208375286No Hit
GCTGGCAACTAAAGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACA253710.37901218706957596No Hit
GGGGTCTTACTCACTTGCGTGATGGGAAATCTTATCTTGAGGGGGGCTTC250080.3735894042109477No Hit
GTCCCAAAGTATACGTTAAGTGGAAAAGGATGTGGAGTTGCTTAGACAAC246680.36851021365465686No Hit
CCTGAACATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGA246440.3681516825565658No Hit
GTTGCTTCGAATTAAACCACATGCTCCGCTGCTTGTGCGGGTCCCCGTCA240440.35918840510428773No Hit
CCCTGAACATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCG215310.32164721137499663No Hit
GTCGTCAACTGCAAAATTCACACCCGCCACTCACTCAAAGTGGGTGACAC214810.32090027158730683No Hit
CCCCGGTCTACCTTCTATTACCTATGAATTCAGTAATAGATACTTGGACA214420.3203176585529087No Hit
GTCCCGTGGTACTCTGGATCATTCTCAAAGTTCTTCAGTGTTTCGCTTAC212390.317285083014888No Hit
GGCGTTACTCAAGCAGTTAACTCAAGTTAGGCTACCCCACGGCACACGAA205510.30700719153627587No Hit
CCTCTCTTCGACATACCGTCATAGGCGTTACTCAAGCAGTTAACTCAAGT202130.3019578785714926No Hit
GCCCTTTGTATCGGCCATTGTAGCACGTGTGTAGCCCTGAACATAAGGGG193360.2888565546954129No Hit
CGGACATTTTCGGCGCAGAATCACTCGACCAGTGAGCTATTACGCACTCT190220.28416577282872074No Hit
CTCCCACCTATCCTGTACATCTAGTACCAAAATCCAATGTCAAGCTGCAG182040.2719458379021151No Hit
CCCACCTATCCTGTACATCTAGTACCAAAATCCAATGTCAAGCTGCAGTA179890.2687339968150487No Hit
GTCCCGTGGTACTCTGGATCATTCTCAAAGATTTTCAGTGTTTCGCTTAC174270.26033839360141503No Hit
GGCCCCTAGACCATTCAGTGCTCTACCTCCGTCACTCTCATTTCAAGGCT159350.23804971033675035No Hit
CTCCGTCACTCTCATTTCAAGGCTAGCCCTAAAGCTATTTCGGCGAGAAC155930.23294064218895186No Hit
GTCCGTCGTGAAGAGGGAAACAACCCAGACCGCCAGCTAAGGTCCCAAAG148890.22242372997827897No Hit
GCCCCCACACCCGCCTGCTGGAAGGTTCGCCCAGGGGTTACCTTCACTAC142300.21257906357652698No Hit
GCTTCCTTCCGGACCTGGCGGGGTTCATGACTTTCCGTTGCGTAGGACCC138600.2070517091476222No Hit
CCCCCTTGTTTTTTGTTCTTCAACATATCGTTTTATAGTTTCACTTGATA138550.20697701516885322No Hit
GTAGCCCTGAACATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCC136360.20370541889877172No Hit
GGCCCGTTCTACAAAAAGTACGCGGTCACGCATTAAAAGCGCTTCCACAG135910.20303317308985086No Hit
CCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCACTCGGTACCAC134030.20022467948813708No Hit
GCTCGCCTACCGAATATTTCTATTCCCATAGCTTCGGTGATACGTTTGAG133020.19871586111700362No Hit
GCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGA128980.19268058763246976No Hit
GCCCGTTCTACAAAAAGTACGCGGTCACGCATTAAAAGCGCTTCCACAGC122870.1835529834269No Hit
CCCGTCAATTCCTTTGAGTTTCACACTTGCGTGCGTACTCCCCAGGCGGA121230.18110302092327735No Hit
GTCCCGACTAACCCTGAGCGGACGAACCTTCCTCAGGAAACCTTAGGTTT120080.17938505941159072No Hit
GGCCCTTTACCTTCAGCTTCTACGAGCGTAGTTACTGTTTTAGATCTCGT116090.17342447990582585No Hit
GGCGGGGTTCATGACTTTCCGTTGCGTAGGACCCAACTATCATCATCACT113960.17024251641026714No Hit
CTTGGTTTGGGCTCTTCCCCTTTCGCTCGCCGCTACTGAGGGAATCGATT111180.16608953119071165No Hit
GTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCC110700.1653724689945294No Hit
CTTCGACATACCGTCATAGGCGTTACTCAAGCAGTTAACTCAAGTTAGGC110450.1649989991006845No Hit
GTCCGCAACCCCAAAAACTTAAGTTCTTGGTTTGGGCTCTTCCCCTTTCG110240.16468528438985477No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT107510.16060699314906826No Hit
GGCCCGTTCTACAAAAAGTACGCGGTCACGCATATATAGCGCTTCCACAG104090.1554979250012698No Hit
GTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTA103130.15406380060890532No Hit
GGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGT102840.15363057553204523No Hit
GCCCGTTCTACAAAAAGTACGCGGTCACGCATATATAGCGCTTCCACAGC97160.1451453395438887No Hit
CTGGGGTCTTACTCACTTGCGTGATGGGAAATCTTATCTTGAGGGGGGCT97060.14499595158635073No Hit
GGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTC92930.1388262289400327No Hit
AGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGT91490.13667504235148595No Hit
CTCTTCGACATACCGTCATAGGCGTTACTCAAGCAGTTAACTCAAGTTAG89680.13397112032004876No Hit
GGTGTGTACAAGACCCGGGAACGCATTCACCGCGACATTCTGATTCGCGA85550.12780139767373072No Hit
GGTCACTAGGTAGTATTTAGCCTTGGGGGGTGGTCCCCCCTGCTTCCCAC84940.12689013113274913No Hit
CCCGGGAACGCATTCACCGCGACATTCTGATTCGCGATTACTAGCAACTC84420.1261133137535517No Hit
GTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGA84020.12551576192339983No Hit
GACCCGGGAACGCATTCACCGCGACATTCTGATTCGCGATTACTAGCAAC83600.12488833250174038No Hit
GCTGCCCTTTGTATCGGCCATTGTAGCACGTGTGTAGCCCTGAACATAAG82840.12375298402445183No Hit
CCCTACTTGGTCTTGCTCCAGATGGGGTTTACATAGCCAACTAGTCACCT82160.12273714591319364No Hit
ACCTCTCTTCGACATACCGTCATAGGCGTTACTCAAGCAGTTAACTCAAG81540.12181094057645825No Hit
GCCCAGTGATTCCGGATAACGCTTGCCCCCTACGTATTACCGCGGCTGCT80700.12055608173313934No Hit
CCCCCACACCCGCCTGCTGGAAGGTTCGCCCAGGGGTTACCTTCACTACT80680.12052620414163173No Hit
GTGGCGAAGGCGGCCCCCTGGACGAAGACTGACGCTCAGGTGCGAAAGCG80500.12025730581806339No Hit
GCTCCCCATCACCTCTCAGGATTATGCTAAAACGGATTTGCCTGTCTTAG80100.11965975398791154No Hit
GGGACCTTAGCTGATGATCTGGGTTCTTTCCCTCTTGACCACGGATCTTA79090.11815093561677807No Hit
GCCCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCC78690.11755338378662619No Hit
CTTCGAATTAAACCACATGCTCCGCTGCTTGTGCGGGTCCCCGTCAATTC78160.11676162761167497No Hit
GCCGAGTTCTGTATTGGATAATCATCTATCTAGGCCAATTGTTGCCAATT77450.1157009731131554No Hit
CCTCCGTCACTCTCATTTCAAGGCTAGCCCTAAAGCTATTTCGGCGAGAA77350.11555158515561743No Hit
GGGGTTTACATAGCCAACTAGTCACCTAGTTGCTGGTGAGCTCTTACCTC76180.11380374605242322No Hit
GCACGTGTGTAGCCCTGAACATAAGGGGCATGATGATTTGACGTCATCCC76010.11354978652460869No Hit
CCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG75980.1135049701373473No Hit
CCCAGTGATTCCGGATAACGCTTGCCCCCTACGTATTACCGCGGCTGCTG75320.1125190096175967No Hit
TGCACCTCTCTTCGACATACCGTCATAGGCGTTACTCAAGCAGTTAACTC73570.1099047203606823No Hit
CACCTATCCTGTACATCTAGTACCAAAATCCAATGTCAAGCTGCAGTAAA73540.1098599039734209No Hit
GTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCCTTTGTTGCCAGCG72460.10824651403201085No Hit
GCACTTTCCTTGGAGTCACCTCCACTGGGTATTACCCAGCACCCTGCCCT72090.10769377858912037No Hit
CCGGTGGCGAAGGCGGCCCCCTGGACGAAGACTGACGCTCAGGTGCGAAA72090.10769377858912037No Hit
CCCGGCAGTCTCTCTAGAGTGCCCATCCGAAATGCTGGCAACTAAAGACA70690.10560234718358884No Hit
ACCCCCTTGTTTTTTGTTCTTCAACATATCGTTTTATAGTTTCACTTGAT67920.10146430075978716No Hit
GCACCCTTTACGCCCAGTGATTCCGGATAACGCTTGCCCCCTACGTATTA67400.10068748338058972No Hit
GCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCG67210.10040364626126759No Hit
CCCCCCTGCTTCCCACAGGGTTTCACGTGTCCCGTGGTACTCTGGATCAT66950.10001523757166887No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)