FastQCFastQC Report
Mon 26 Jun 2023
ERR3804293_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3804293_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136831370
Total Bases6.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGT71760205.244426040607501No Hit
GATCCATTAGCTCAGTCGGTAGAGCACCTGAGATCGGAAGAGCACACGTC37619452.7493293387327777No Hit
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAG30186142.206083298003959No Hit
TCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTCGGAG17738561.2963810857115585No Hit
GGGGGCGTAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC17711301.2943888524977862TruSeq Adapter, Index 1 (100% over 36bp)
TCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTCGAGA17690641.2928789648163284No Hit
GGGGGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC13268310.9696833408888619TruSeq Adapter, Index 1 (100% over 36bp)
AGCGTGAGGTCGGTGGTTCGAGTCCACTTGAGCCCAGATCGGAAGAGCAC10650920.7783975268244409No Hit
AAGAACCTTACCTGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA8540070.6241310015386091Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGGCAGATCGGAAGAGCACACG6478550.4734696436935477No Hit
GATCCATTAGCTCAGTCGGTAGAGCACCTGACTTTAGATCGGAAGAGCAC5985760.4374552414406141No Hit
GATCCATTAGCTCAGTCGGTAGAGCACCTGACAGATCGGAAGAGCACACG5507750.40252100084943976No Hit
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTAGATCGGAAGAGC5416550.39585586258472744No Hit
GGCGGCATAGCTCAGCTGGCTAGAGCGTTCGGAGATCGGAAGAGCACACG5413800.3956548852795963No Hit
GCCGAAGTGGCGGAACTGGCAGACGCAGATCGGAAGAGCACACGTCTGAA4971240.36331142485820317Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGCGTAGCTCAGTTGGGAGAGCACCTGCAGATCGGAAGAGCACACGT4720070.3449552540473723No Hit
TAGGTCTCTGCAAAGTCGAAAGACGACGTAGATCGGAAGAGCACACGTCT4470730.32673282449777413Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAACCTTACCTGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC4467200.3264748427206422Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGGATTGTGGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA4391030.32090813678179203Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAGATCGGAAGAGCACACG4304980.3146193741975981No Hit
TAGGTCTCTGCAAAGTCGAAAGACGACGTATAGATCGGAAGAGCACACGT4281530.3129055859047527No Hit
AAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTC3914910.2861120224112351No Hit
GATCCATCAGCTCAGTCGGTAGAGCACCTGAGATCGGAAGAGCACACGTC3869940.2828254953524181No Hit
AGGGGTGTAGTTCAGTTGGTAGAACGTCGGTCAGATCGGAAGAGCACACG3645230.2664030916302307No Hit
ACGCGTGGGTAACCTACCTTGTACAGGGGGAGATCGGAAGAGCACACGTC3473780.25387307018851013No Hit
TTCGGATTGTGGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3392290.2479175645175518Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TACCGGTTCGATTCCGGTCTTCGGCACCAGATCGGAAGAGCACACGTCTG3259010.23817710807105125Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGAGGCAAATATCTAACAGATCGGAAGAGCACACGTCTGAACTCCAGT3156250.23066713429822416Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGTAGAGCAGTTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGTCT3128990.2286749010844516Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACGACGTATAGGGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGT3024940.2210706506848539Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGGCGTAGCTCAGTTGGGAGAGCACCTGAGATCGGAAGAGCACACGTC2983170.21801798812655315No Hit
TTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2890190.21122276273342874Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGACGTGGTAAGCTGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC2741090.20032613866250115Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCGTGAGGTCGGTGGTTCGAGTCCACTTGAGCAGATCGGAAGAGCACAC2481190.18133195626119947No Hit
TAGGTCTCTGCAAAGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC2394760.1750154222675692Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGGTACCTCGGGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2364380.17279517116579335Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACCCATTAGCTCAGTCGGTAGAGCACCTGAGATCGGAAGAGCACACGTC2364160.17277909298138286No Hit
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGAGATCGGAAGAGCACACGTC2325420.16994787087200836No Hit
ATAGGTCTCTGCAAAGTCGAAAGACGACGTAGATCGGAAGAGCACACGTC2323860.1698338619280067No Hit
ATCAGAAGGTCGGGAGTTCGAATCTCTCTGGGCGCAGATCGGAAGAGCAC2313410.16907014816850843No Hit
ACCGGTAGGTTGGAGGTTCGAGTCCTCTCTCTGGAGATCGGAAGAGCACA2142390.15657155226904473No Hit
ACGGCGGTAACAGGGGTTCGATTCCCCTAGGGGAGATCGGAAGAGCACAC2099790.15345823110592258No Hit
TACCGGTTCGATTCCGGTCTTCGGCAGATCGGAAGAGCACACGTCTGAAC2022700.14782428912317402Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGCAGTTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGTCTGAACTC2000540.1462047774570992Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCAGAAGGTCGGGAGTTCGAATCTCTCTGGGCGCACCAGATCGGAAGAG1952330.14268146259150954No Hit
TCGGTCTCAGTTCGGATTGTGGGCTGAGATCGGAAGAGCACACGTCTGAA1941000.14185343609436932Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGGGGCAGAGCAGTTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGT1823880.13329399537547565No Hit
GCCGAAGCGGCGGAACTGGCAGACGCAGATCGGAAGAGCACACGTCTGAA1745870.1275928173488287Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGAAGATCGGAAGAGCACACGT1735030.12680060135333002No Hit
ACCGGTAGGTTGGAGGTTCGAGTCCTCTCTCTGGAGCCAGATCGGAAGAG1718680.12560569992100495No Hit
GCGGAAGTGGCTCAGTGGTAGAGCATCGCCAGATCGGAAGAGCACACGTC1710820.125031270241612No Hit
CGGGGCGTAGTTCAGTTTGGTAGAACGCATGACAGATCGGAAGAGCACAC1703080.12446561048098839No Hit
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACAGATCGGAAGAGCA1653840.12086702047929507No Hit
TCCAGAGTAGCTCAATGGTGGAGCACCCGGCTGTAGATCGGAAGAGCACA1598700.11683724280477496No Hit
AGGTCTCTGCAAAGTCGAAAGACGACGTATAGATCGGAAGAGCACACGTC1591490.11631031685204936No Hit
AGCGTGAGGTCGGTGGTTCGAGTCCACTTGAGCCAGATCGGAAGAGCACA1591470.11630885519892113No Hit
AAGACGAGCGGGAGAACCATTGTTAAGGAACTAGATCGGAAGAGCACACG1572750.11494074787090124No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG1488310.1087696483635295TruSeq Adapter, Index 1 (100% over 49bp)
AGCGTGAGGTCGGTGGTTCGAGTCCACTTGAGCCTAGATCGGAAGAGCAC1473750.10770556488618072No Hit
ACACTTCGGTCGCAGAGAACAGGCCCAAGATCGGAAGAGCACACGTCTGA1381230.10094395751500551Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGCAGATCGGAAGAGCACACGT1379280.10080144633500343No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)