FastQCFastQC Report
Fri 23 Jun 2023
ERR3804294_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3804294_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33584417
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGT16794865.000789503060303No Hit
GATCCATTAGCTCAGTCGGTAGAGCACCTGAGATCGGAAGAGCACACGTC9645082.8718914489419305No Hit
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAG7876652.345328787455206No Hit
TCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTCGGAG4531171.3491882261943091No Hit
TCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTCGAGA4519771.3457937947828602No Hit
GGGGGCGTAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC4110141.2238235369695416TruSeq Adapter, Index 1 (100% over 36bp)
GGGGGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC3078570.916666202661788TruSeq Adapter, Index 1 (100% over 36bp)
AGCGTGAGGTCGGTGGTTCGAGTCCACTTGAGCCCAGATCGGAAGAGCAC2592880.7720485366769952No Hit
AAGAACCTTACCTGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2143280.6381769259237103Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GATCCATTAGCTCAGTCGGTAGAGCACCTGACTTTAGATCGGAAGAGCAC1546740.4605528808196968No Hit
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGGCAGATCGGAAGAGCACACG1521290.45297496157220773No Hit
GATCCATTAGCTCAGTCGGTAGAGCACCTGACAGATCGGAAGAGCACACG1426270.4246820780006394No Hit
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTAGATCGGAAGAGC1416890.4218891160147279No Hit
GGCGGCATAGCTCAGCTGGCTAGAGCGTTCGGAGATCGGAAGAGCACACG1264020.37637098181576295No Hit
TAGGTCTCTGCAAAGTCGAAAGACGACGTAGATCGGAAGAGCACACGTCT1167160.3475302251041011Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAAGTGGCGGAACTGGCAGACGCAGATCGGAAGAGCACACGTCTGAA1162110.34602655154025747Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAGATCGGAAGAGCACACG1126550.3354383075936676No Hit
AAGAACCTTACCTGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC1120830.333735136745116Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAGGTCTCTGCAAAGTCGAAAGACGACGTATAGATCGGAAGAGCACACGT1114490.33184735646892427No Hit
GGGGGCGTAGCTCAGTTGGGAGAGCACCTGCAGATCGGAAGAGCACACGT1097460.3267765523516457No Hit
TCGGATTGTGGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1074020.3197971249582805Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCCATCAGCTCAGTCGGTAGAGCACCTGAGATCGGAAGAGCACACGTC992160.29542272536694625No Hit
AAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTC935480.27854585059493514No Hit
AGGGGTGTAGTTCAGTTGGTAGAACGTCGGTCAGATCGGAAGAGCACACG877100.2611627886826203No Hit
ACGCGTGGGTAACCTACCTTGTACAGGGGGAGATCGGAAGAGCACACGTC837650.24941626945615877No Hit
TTCGGATTGTGGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC820750.2443841737672564Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TACCGGTTCGATTCCGGTCTTCGGCACCAGATCGGAAGAGCACACGTCTG799760.23813425137021138Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGAGGCAAATATCTAACAGATCGGAAGAGCACACGTCTGAACTCCAGT757250.22547659529120306Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGACGACGTATAGGGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGT741080.22066186231548995Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGTAGAGCAGTTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGTCT739440.22017354060366745Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC727060.21648730719369047Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGGCGTAGCTCAGTTGGGAGAGCACCTGAGATCGGAAGAGCACACGTC698390.2079506099510377No Hit
AGGACGTGGTAAGCTGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC667760.1988303087113288Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAGGTCTCTGCAAAGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC623140.18554438506406112Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCGTGAGGTCGGTGGTTCGAGTCCACTTGAGCAGATCGGAAGAGCACAC593420.17669504282298543No Hit
ACGGTACCTCGGGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC577510.17195772670402465Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATAGGTCTCTGCAAAGTCGAAAGACGACGTAGATCGGAAGAGCACACGTC576990.17180289299051998No Hit
GACCCATTAGCTCAGTCGGTAGAGCACCTGAGATCGGAAGAGCACACGTC575330.1713086161358704No Hit
ATCAGAAGGTCGGGAGTTCGAATCTCTCTGGGCGCAGATCGGAAGAGCAC570880.1699835968568399No Hit
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGAGATCGGAAGAGCACACGTC550010.1637694053167575No Hit
ACCGGTAGGTTGGAGGTTCGAGTCCTCTCTCTGGAGATCGGAAGAGCACA511610.15233553108871892No Hit
ACGGCGGTAACAGGGGTTCGATTCCCCTAGGGGAGATCGGAAGAGCACAC502010.14947706253170928No Hit
TACCGGTTCGATTCCGGTCTTCGGCAGATCGGAAGAGCACACGTCTGAAC496540.1478483309684965Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGTCTCAGTTCGGATTGTGGGCTGAGATCGGAAGAGCACACGTCTGAA494190.14714860168631186Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGCAGTTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGTCTGAACTC480600.14310208213529507Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCAGAAGGTCGGGAGTTCGAATCTCTCTGGGCGCACCAGATCGGAAGAG480170.14297404656451235No Hit
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACAGATCGGAAGAGCA434670.12942609663285207No Hit
CGCGGGGCAGAGCAGTTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGT425440.12667779821814384No Hit
GCCGAAGCGGCGGAACTGGCAGACGCAGATCGGAAGAGCACACGTCTGAA413940.1232535910925594Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGAAGATCGGAAGAGCACACGT413400.1230928022362276No Hit
ACCGGTAGGTTGGAGGTTCGAGTCCTCTCTCTGGAGCCAGATCGGAAGAG410730.1222977906688093No Hit
CGGGGCGTAGTTCAGTTTGGTAGAACGCATGACAGATCGGAAGAGCACAC407700.12139558653050313No Hit
GCGGAAGTGGCTCAGTGGTAGAGCATCGCCAGATCGGAAGAGCACACGTC397970.11849840954511731No Hit
TCCAGAGTAGCTCAATGGTGGAGCACCCGGCTGTAGATCGGAAGAGCACA389550.11599129441490677No Hit
AAGACGAGCGGGAGAACCATTGTTAAGGAACTAGATCGGAAGAGCACACG383120.11407671599599303No Hit
AGCGTGAGGTCGGTGGTTCGAGTCCACTTGAGCCAGATCGGAAGAGCACA382980.1140350299962033No Hit
AGGTCTCTGCAAAGTCGAAAGACGACGTATAGATCGGAAGAGCACACGTC382340.11384446542573598No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG363810.10832702559642467TruSeq Adapter, Index 1 (100% over 49bp)
AGCGTGAGGTCGGTGGTTCGAGTCCACTTGAGCCTAGATCGGAAGAGCAC356410.10612362275039641No Hit
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACAGATCG351900.1047807380428846No Hit
ACACTTCGGTCGCAGAGAACAGGCCCAAGATCGGAAGAGCACACGTCTGA337520.10049899035019724Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)