FastQCFastQC Report
Sat 24 Jun 2023
ERR3804295_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3804295_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150136426
Total Bases7.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAG31649852.1080726938311427No Hit
TAGGTCTCTGCAAAGTCGAAAGACGACGTAGATCGGAAGAGCACACGTCT23597471.5717351630576315Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGTCTCTGCAAAGTCGAAAGACGACGTATAGATCGGAAGAGCACACGT21245861.415103620489807No Hit
GATCCATTAGCTCAGTCGGTAGAGCACCTGAGATCGGAAGAGCACACGTC16279791.0843331251271426No Hit
AGGTCTCTGCAAAGTCGAAAGACGACGTATAGATCGGAAGAGCACACGTC14844600.9887407337110848No Hit
AGGTCTCTGCAAAGTCGAAAGACGACGTAGATCGGAAGAGCACACGTCTG12378440.8244794637645098Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAGGTCTCTGCAAAGTCGAAAGACGACGTAGATCGGAAGAGCACACGTC11536030.7683698291845578No Hit
AAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTC11221170.7473982363214108No Hit
TCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTCGGAG10533380.7015872350657928No Hit
TCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTCGAGA8121440.540937347209797No Hit
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTAGATCGGAAGAGC7561170.5036199542941031No Hit
AAGAACCTTACCTGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5609180.3736055366070856Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCGAGGCAAATATCTAACAGATCGGAAGAGCACACGTCTGAACTCCAGT4938450.328930835212502Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGGCAGATCGGAAGAGCACACG4210400.28043827285458356No Hit
AAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTAGATCGGAAGAGCAC4005810.26681133331360907No Hit
AGACGACGTATAGGGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGT3973700.26467261182839136Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAAACATAGGTCTCTGCAAAGTCGAAAGACGACGTAGATCGGAAGAGCA3512790.23397319981494696No Hit
GGGGGCGTAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA3481100.23186245288668322TruSeq Adapter, Index 2 (100% over 36bp)
AGGACGTGGTAAGCTGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC3457730.23030586861045965Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAGGTCTCTGCAAAGTCGAAAGACGACGCAGATCGGAAGAGCACACGTCT3252430.21663163874701533Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGTGTAGTTCAGTTGGTAGAACGTCGGTCAGATCGGAAGAGCACACG3045530.20285083914279406No Hit
GGGGGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA2954740.19680367241458113TruSeq Adapter, Index 2 (100% over 36bp)
TAGGTCTCTGCAAAGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC2881180.19190412858236017Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGT2527430.16834222495745302No Hit
ACATAGGTCTCTGCAAAGTCGAAAGACGACGTAGATCGGAAGAGCACACG2228470.1484296688932771No Hit
TTCCGGTCTTCGGCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2140730.14258565073342028Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGACGACGTATAGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2123390.14143070116774992Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GATCCATTAGCTCAGTCGGTAGAGCACCTGACAGATCGGAAGAGCACACG2098530.13977487382042783No Hit
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACAGATCGGAAGAGCA2085700.13892031771157254No Hit
ACGAGCGGGAGAACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC2009170.13382295379803433Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGGTCTCTGCAAAGTCGAAAGACGACGTACAGATCGGAAGAGCACACGT1991830.13266800423236397No Hit
ATAGGTCTCTGCAAAGTCGAAAGACGACGTATAGATCGGAAGAGCACACG1962980.1307464185939793No Hit
AGGTCTCTGCAAAGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1951520.12998311282566433Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGGTACCTCGGGAGGAAGCCCCGGCAGATCGGAAGAGCACACGTCTGAA1892580.12605734999979284Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGAACCTTACCTGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC1866110.12429428685081395Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATAGGTCGAGGACTTGACCAGATCGGAAGAGCACACGTCTGAACTCCAG1839960.12255253764999043Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCGGTAGGTTGGAGGTTCGAGTCCTCTCTCTGGAGCCAGATCGGAAGAG1802180.12003615964589433No Hit
TCCAGAGTAGCTCAATGGTGGAGCACCCGGCTGTAGATCGGAAGAGCACA1767270.11771094111431692No Hit
GATCCATTAGCTCAGTCGGTAGAGCACCTGACTTTAGATCGGAAGAGCAC1760360.1172506930463364No Hit
AGGTCTCTGCAAAGTCGAAAGACGACGCAGATCGGAAGAGCACACGTCTG1702010.11336422781237646Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACCAGATCGGAAGAGC1696770.1130152119113319No Hit
AATGACGGTACCTCGGGAGGAAGCCCCGGCAGATCGGAAGAGCACACGTC1638870.10915871941696548No Hit
ACGCGTGGGTAACCTACCTTGTACAGGGGGAGATCGGAAGAGCACACGTC1610290.10725511742233694No Hit
ATAGGTCTCTGCAAAGTCGAAAGACGACGCAGATCGGAAGAGCACACGTC1582940.10543344091593068No Hit
AGAATGTCGCGGTGAATGCGAGATCGGAAGAGCACACGTCTGAACTCCAG1581040.10530688934875805Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATCCATCAGCTCAGTCGGTAGAGCACCTGAGATCGGAAGAGCACACGTC1545610.10294703565142813No Hit
ACGACGTATAGGGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1540240.10258936095894544Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCAGAAGGTCGGGAGTTCGAATCTCTCTGGGCGCACCAGATCGGAAGAG1510750.10062514742425No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)