FastQCFastQC Report
Thu 22 Jun 2023
ERR3804296.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3804296.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37290737
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAG8320222.2311760692742544No Hit
TAGGTCTCTGCAAAGTCGAAAGACGACGTAGATCGGAAGAGCACACGTCT6186161.6588999032118887Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGTCTCTGCAAAGTCGAAAGACGACGTATAGATCGGAAGAGCACACGT5565001.4923277059394133No Hit
GATCCATTAGCTCAGTCGGTAGAGCACCTGAGATCGGAAGAGCACACGTC4211541.1293796633732394No Hit
AGGTCTCTGCAAAGTCGAAAGACGACGTATAGATCGGAAGAGCACACGTC3589020.9624427642714597No Hit
AGGTCTCTGCAAAGTCGAAAGACGACGTAGATCGGAAGAGCACACGTCTG2999880.8044571497742188Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAGGTCTCTGCAAAGTCGAAAGACGACGTAGATCGGAAGAGCACACGTC2880460.7724331112039968No Hit
TCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTCGGAG2695980.7229623807113278No Hit
AAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTC2683380.7195835255280688No Hit
TCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTCGAGA2084250.558918961564101No Hit
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTAGATCGGAAGAGC1991500.5340468331317775No Hit
AAGAACCTTACCTGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1406980.3773001322017315Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCGAGGCAAATATCTAACAGATCGGAAGAGCACACGTCTGAACTCCAGT1198540.32140421359867466Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGGCAGATCGGAAGAGCACACG1005990.2697694068100612No Hit
AGACGACGTATAGGGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGT977830.262217933638587Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTAGATCGGAAGAGCAC959250.2572354630588288No Hit
AAAAACATAGGTCTCTGCAAAGTCGAAAGACGACGTAGATCGGAAGAGCA883900.23702937273672012No Hit
TAGGTCTCTGCAAAGTCGAAAGACGACGCAGATCGGAAGAGCACACGTCT852900.2287163163334637Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGACGTGGTAAGCTGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC845710.2267882235741278Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGCGTAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA810210.21726843317685032TruSeq Adapter, Index 2 (100% over 36bp)
TAGGTCTCTGCAAAGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC760160.203846869532238Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGGGGTGTAGTTCAGTTGGTAGAACGTCGGTCAGATCGGAAGAGCACACG741590.19886708058357763No Hit
GGGGGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA697760.18711349148181222TruSeq Adapter, Index 2 (100% over 36bp)
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGT606090.16253097920805373No Hit
GATCCATTAGCTCAGTCGGTAGAGCACCTGACAGATCGGAAGAGCACACG550930.14773910207245303No Hit
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACAGATCGGAAGAGCA549560.14737171861205103No Hit
ACATAGGTCTCTGCAAAGTCGAAAGACGACGTAGATCGGAAGAGCACACG548520.14709282897787726No Hit
TTCCGGTCTTCGGCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA526900.1412951425443804Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGGTCTCTGCAAAGTCGAAAGACGACGTACAGATCGGAAGAGCACACGT525390.14089021624860887No Hit
AGACGACGTATAGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA523200.14030293903818528Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATAGGTCTCTGCAAAGTCGAAAGACGACGTATAGATCGGAAGAGCACACG492170.1319818377416354No Hit
ACGAGCGGGAGAACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC490290.13147769109524438Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGTCTCTGCAAAGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA472200.12662662043927958Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGAACCTTACCTGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC472100.12659980412830135Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGGTACCTCGGGAGGAAGCCCCGGCAGATCGGAAGAGCACACGTCTGAA466840.12518926617084558Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCCATTAGCTCAGTCGGTAGAGCACCTGACTTTAGATCGGAAGAGCAC450670.12085306868566315No Hit
AATAGGTCGAGGACTTGACCAGATCGGAAGAGCACACGTCTGAACTCCAG449660.12058222394478287Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACCAGATCGGAAGAGC447200.119922542694718No Hit
TCCAGAGTAGCTCAATGGTGGAGCACCCGGCTGTAGATCGGAAGAGCACA435000.11665095275537193No Hit
ACCGGTAGGTTGGAGGTTCGAGTCCTCTCTCTGGAGCCAGATCGGAAGAG433780.11632379376143732No Hit
AGGTCTCTGCAAAGTCGAAAGACGACGCAGATCGGAAGAGCACACGTCTG414890.11125819261764656Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGACGGTACCTCGGGAGGAAGCCCCGGCAGATCGGAAGAGCACACGTC404040.10834862287650683No Hit
ATAGGTCTCTGCAAAGTCGAAAGACGACGCAGATCGGAAGAGCACACGTC399790.10720892965993137No Hit
GATCCATCAGCTCAGTCGGTAGAGCACCTGAGATCGGAAGAGCACACGTC398040.10673964421781204No Hit
ACGCGTGGGTAACCTACCTTGTACAGGGGGAGATCGGAAGAGCACACGTC393880.10562408568111699No Hit
AGAATGTCGCGGTGAATGCGAGATCGGAAGAGCACACGTCTGAACTCCAG384660.10315162180892269Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCAGAAGGTCGGGAGTTCGAATCTCTCTGGGCGCACCAGATCGGAAGAG375250.10062820694586969No Hit
ACGACGTATAGGGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA374780.10050217028427194Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)