FastQCFastQC Report
Sat 24 Jun 2023
ERR3804297.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3804297.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175009976
Total Bases8.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTC53963133.0834316553474643No Hit
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAG37459082.140396842292007No Hit
AAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTAGATCGGAAGAGCAC30541271.745115946990359No Hit
AATGACGGTACCTCGGGAGGAAGCCCCGGCAGATCGGAAGAGCACACGTC21808941.24615410495228No Hit
TCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTCGGAG21602491.2343576345613578No Hit
TAGGTCTCTGCAAAGTCGAAAGACGACGTAGATCGGAAGAGCACACGTCT16939440.9679128234381336Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTCTCTGCAAAGTCGAAAGACGACGTAGATCGGAAGAGCACACGTCTG14980250.8559654907900792Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTCGGATTGTGGGCTGAAACTAGATCGGAAGAGCACACGTCTGAACTC14690610.8394155770868742Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTAGATCGGAAGAGC13776170.7871648413916701No Hit
GATCCATTAGCTCAGTCGGTAGAGCACCTGAGATCGGAAGAGCACACGTC13656050.7803012326565887No Hit
AGAATGTCGCGGTGAATGCGAGATCGGAAGAGCACACGTCTGAACTCCAG12509990.7148158228420076Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATAGGTCTCTGCAAAGTCGAAAGACGACGTAGATCGGAAGAGCACACGTC12353550.7058769038400416No Hit
AGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTCGGAGTAGATCGG12216730.6980590637873123No Hit
TCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTCGAGA12094770.69109031818849No Hit
TAGGTCTCTGCAAAGTCGAAAGACGACGTATAGATCGGAAGAGCACACGT11530290.6588361568599952No Hit
AGGTCTCTGCAAAGTCGAAAGACGACGTATAGATCGGAAGAGCACACGTC11476360.6557546182395911No Hit
AAGAACCTTACCTGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA9589200.5479230509693916Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACAGATCGGAAGAGCACA9486910.5420782412997988No Hit
AAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACCAGATCGGAAGAGCAC9119710.5210965802315177No Hit
AGCGTGAGGTCGGTGGTTCGAGTCCACTTGAGCCCAGATCGGAAGAGCAC7461900.42636998018901506No Hit
CGGTCTCAGTTCGGATTGTGGGCTGAAACTAGATCGGAAGAGCACACGTC7458470.4261739913614982No Hit
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGT7131160.4074716289315988No Hit
AAAAACATAGGTCTCTGCAAAGTCGAAAGACGACGTAGATCGGAAGAGCA6388020.36500890669226765No Hit
AAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTA6298390.3598874843568918No Hit
AATGACGGTACCTCGGGAGGAAGCCCCGGCTAGATCGGAAGAGCACACGT5892740.3367088056740263No Hit
CGGTCTCAGTTCGGATTGTGGGCTGAAACAGATCGGAAGAGCACACGTCT5064670.2893932172186573Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTCTCAGTTCGGATTGTGGGCTGAAACTAGATCGGAAGAGCACACGTCT4831080.2760459780875577Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTCGGAGTTGCTAGT4704460.2688109619533917No Hit
AGTTCGGATTGTGGGCTGAAACCAGATCGGAAGAGCACACGTCTGAACTC4341570.24807557256050364Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTTCGGATTGTGGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4312410.24640938182861075Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTCGGAGTTGCTAGA4169220.23822756252477859No Hit
GGGGGCGTAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA4058290.23188906671240272TruSeq Adapter, Index 3 (100% over 36bp)
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACCAGATCGGAAGAGC4032530.23041715062003093No Hit
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACAGATCGGAAGAGCA4011350.2292069338950141No Hit
AATAGGTCGAGGACTTGACCAGATCGGAAGAGCACACGTCTGAACTCCAG3885780.22203191434070021Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTGGTACAAGATGGGCCCGCGTCTGATTAGATCGGAAGAGCACACGTCT3854390.2202383023011214Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTCTCAGTTCGGATTGTGGGCTGAAACAGATCGGAAGAGCACACGTCTG3770910.21546828850487926Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGTCTCAGTTCGGATTGTGGGCTGAAACTAGATCGGAAGAGCACACGT3620830.2068927773580176No Hit
TAGGTCTCTGCAAAGTCGAAAGACGACGCAGATCGGAAGAGCACACGTCT3498230.19988746241528538Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAATGTCGCGGTGAATGCAGATCGGAAGAGCACACGTCTGAACTCCAGT3393800.19392037400199402Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATGTTCAGGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG3315870.18946748498497024TruSeq Adapter, Index 3 (100% over 37bp)
AGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTCGGAGCAGATCGG3257140.18611167628524217No Hit
AAGAACCTTACCTGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC3216260.18377580944299998Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGAGTTGCTAGTAATCGCGAATCAGATCGGAAGAGCACACGTCTGAACT3163380.18075426740244796Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGTCTCTGCAAAGTCGAAAGACGACGCAGATCGGAAGAGCACACGTCTG3110080.17770872672995508Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA3096460.17693048537987344TruSeq Adapter, Index 3 (100% over 36bp)
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGGCAGATCGGAAGAGCACACG3070290.17543514205155938No Hit
TCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTCAGAT2854890.1631272722418978No Hit
TACCGGTTCGATTCCGGTCTTCGGCACCAGATCGGAAGAGCACACGTCTG2589420.14795842266728843Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAGGTCTCTGCAAAGTCGAAAGACGACGCAGATCGGAAGAGCACACGTC2564700.14654593175877015No Hit
GGGGGCGTAGCTCAGTTGGGAGAGCACCTGCAGATCGGAAGAGCACACGT2497020.14267872364030265No Hit
AACCGTAGTAAGCCTTAGAAACTTAGATCGGAAGAGCACACGTCTGAACT2495240.14257701515255336Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGTACCTCGGGAGGAAGCCCCGGCAGATCGGAAGAGCACACGTCTGAA2419720.13826183257119012Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGACGACGTATAGGGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGT2380610.13602710282069863Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCGAGGCAAATATCTAACAGATCGGAAGAGCACACGTCTGAACTCCAGT2356650.13465803800807333Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGTGGGAGACTGAGAACCTAGATCGGAAGAGCACACGTCTGAACTCCAG2329090.13308327063595507Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGTGGGAGACTGAGAACCTAGGACGCCAGATCGGAAGAGCACACGTCTG2299070.1313679398481833Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCCATTAGCTCAGTCGGTAGAGCACCTGACAGATCGGAAGAGCACACG2272660.12985888301590306No Hit
ATCAGAAGGTCGGGAGTTCGAATCTCTCTGGGCGCACCAGATCGGAAGAG2239410.12795899132058622No Hit
TTCCGGTCTTCGGCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2224340.12709789755070877Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCGGTAGGTTGGAGGTTCGAGTCCTCTCTCTGGAGCCAGATCGGAAGAG2205710.12603338680533274No Hit
AAAAACATAGGTCTCTGCAAAGTCGAAAGACGACGTATAGATCGGAAGAG2205410.126016244925375No Hit
CGGTCTCAGTTCGGATTGTGGGCTGAAACCAGATCGGAAGAGCACACGTC2159610.12339925125182578No Hit
AATCTGGAGCGAATCTCATAAAGTCGGTCTCAGATCGGAAGAGCACACGT2082860.11901378696263576No Hit
GCCGAAGTGGCGGAACTGGCAGACGCAGATCGGAAGAGCACACGTCTGAA1965320.1122975983951909Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGGTAGGTTGGAGGTTCGAGTCCTCTCTCTGGAGCAGATCGGAAGAGC1941700.11094796104651772No Hit
CGGTCTCAGTTCGGATTGTGGGCTAGATCGGAAGAGCACACGTCTGAACT1926920.11010343776059943Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAGTCGGTCTCAGTTCGGATTGTGGGCTAGATCGGAAGAGCACACGTCT1897920.10844638936468398Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGTCTCAGTTCGGATTGTGGGCTGAAACAGATCGGAAGAGCACACGTC1879680.10740416306325304No Hit
AGTTCGGATTGTGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1863580.10648421550552066Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAAGGGCAGCGAAGGAGTAATCTGGAGCGAATCTCATAAAGTCGGTCTCA1854080.10594138930685872No Hit
GACGGTACCTCGGGAGGAAGCCCCGGCAGATCGGAAGAGCACACGTCTGA1851080.10576997050728125Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGACGGTACCTCGGGAGGAAGCCCCGGCCAGATCGGAAGAGCACACGT1837390.10498772938520944No Hit
AATGGCCGATACAAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1830160.10457461007822777Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGACTGAGAACCTAGGACGCCAGATCGGAAGAGCACACGTCTGAACTCCA1817460.10384893716001652Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGACCCCAGAAAGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1791680.10237587827564756Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGCGTGGGTAACCTACCTTGTACAGGGGGAGATCGGAAGAGCACACGTC1787860.10215760500418558No Hit
AGGTGGGAGACTGAGAACCTAGGACGCAGATCGGAAGAGCACACGTCTGA1772260.1012662272463828Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATCCATTAGCTCAGTCGGTAGAGCACCTGACTTTAGATCGGAAGAGCAC1766850.10095710201114477No Hit
AGGGGTGTAGTTCAGTTGGTAGAACGTCGGTCAGATCGGAAGAGCACACG1758850.10049998521227156No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)