FastQCFastQC Report
Fri 23 Jun 2023
ERR3804299.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3804299.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115674267
Total Bases5.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGT53185714.597886062247535No Hit
AAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTC22014311.9031294142542523No Hit
AAGACCCCAGAAAGACTATCTGGTAGATAGGTCGGAGGTGTAGATCGGAA14632101.2649399369005727No Hit
AATGACGGTACCTCGGGAGGAAGCCCCGGCAGATCGGAAGAGCACACGTC14070921.2164261218097885No Hit
AAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTAGATCGGAAGAGCAC11901691.0288969455929209No Hit
AGCGTGAGGTCGGTGGTTCGAGTCCACTTGAGCCCAGATCGGAAGAGCAC11460970.9907968554492764No Hit
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAG10604260.9167345750286882No Hit
AAGACGAGCGGGAGAACCATTGTTAAGGAACTAGATCGGAAGAGCACACG10559250.9128434762417816No Hit
AATAGGTCGAGGACTTGACCAGATCGGAAGAGCACACGTCTGAACTCCAG9985040.8632032221998001Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA9328710.8064637228260976TruSeq Adapter, Index 4 (100% over 36bp)
ACGGCGGTAACAGGGGTTCGATTCCCCTAGGGGAGATCGGAAGAGCACAC8638550.746799631762525No Hit
GGGGGCGTAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA6948550.6006997217453731TruSeq Adapter, Index 4 (100% over 36bp)
AGACGAGCGGGAGAACCATTGTTAAGGAACTAGATCGGAAGAGCACACGT6865880.5935529291056584No Hit
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTAGATCGGAAGAGC5712770.493866972158985No Hit
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGCAGATCGGAAGAGCACACGT5568400.48138623605888076No Hit
AGGCGAGCATTCTATGTGGGTTGAAGCTATACCGTAAGGAGTAGTGGACT5547870.47961142472595053No Hit
GCCGAAGTGGCGGAACTGGCAGACGCAGATCGGAAGAGCACACGTCTGAA5437600.4700786217214586Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAATGTCGCGGTGAATGCGAGATCGGAAGAGCACACGTCTGAACTCCAG5307850.45886177951747903Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTCGGTAGGCGAGCATTCTATGTGGGTTGAAGCTATACCGTAAGGAGTA5002560.4324695655949132No Hit
AATGACGGTACCTCGGGAGGAAGCCCCGGCTAGATCGGAAGAGCACACGT4452740.38493781853832715No Hit
ATAGGTCTCTGCAAAGTCGAAAGACGACGTAGATCGGAAGAGCACACGTC4053540.3504271178999561No Hit
AAGACCCCAGAAAGACTATCTGGTAGATAGGTCGGAGGTGCAGATCGGAA4002420.3460078117460645No Hit
AAAAACATAGGTCTCTGCAAAGTCGAAAGACGACGTAGATCGGAAGAGCA3851490.3329599659360712No Hit
AGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTCGGAGTAGATCGG3674010.3176168818947433No Hit
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGGCAGATCGGAAGAGCACACG3601980.31138991354058027No Hit
AAGACGAGCGGGAGAACCAGATCGGAAGAGCACACGTCTGAACTCCAGTC3583430.30978627251642754Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATTTTCAGGAGTCAGACTACGAGTAGATCGGAAGAGCACACGTCTGAACT3479910.30083700465549523Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGTGGAGGTCCGAACCAGTTGACGTTAGATCGGAAGAGCACACGTCTGA3389600.2930297366829219Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGCGTAGCTCAGTTGGGAGAGCACCTGCAGATCGGAAGAGCACACGT3385650.29268826056187586No Hit
AAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACAGATCGGAAGAGCACA3300100.2852924929275757No Hit
AAGAGGGAAAGAACCCAGATCAGATCGGAAGAGCACACGTCTGAACTCCA3276430.2832462296908266Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCTACCCATGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG3100570.268043194083953TruSeq Adapter, Index 4 (100% over 35bp)
AAGACCCCAGAAAGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3022410.261286289369787Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGTTCGGATTGTGGGCTGAAACTAGATCGGAAGAGCACACGTCTGAACTC2981790.2577747045503215Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCGGCATAGCTCAGCTGGCTAGAGCGTTCGGAGATCGGAAGAGCACACG2807700.24272468482553688No Hit
AAGACGAGCGGGAGAACCATTGTTAAGGAACTCAGATCGGAAGAGCACAC2766920.23919926806192773No Hit
AGGTCTCTGCAAAGTCGAAAGACGACGTAGATCGGAAGAGCACACGTCTG2759610.23856732111386536Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAGGGTGCGTAGGCGGCCAATAAAGTCTAGGGTGAAAGGCTACGGCTCA2691790.23270430578998177No Hit
ACCGGTAGGTTGGAGGTTCGAGTCCTCTCTCTGGAGATCGGAAGAGCACA2627970.22718708906968912No Hit
AAGCTGACCGATACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2615160.22607966904168927Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCAGAAGGTCGGGAGTTCGAATCTCTCTGGGCGCAGATCGGAAGAGCAC2568480.22204419933778358No Hit
AGAGCAGTTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGTCTGAACTC2474150.21388940376860138Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGACGAGCGGGAGAACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2430930.2101530498568018Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGGGGTAGAGCAGTTGGTAGCTCGTCCGGAGATCGGAAGAGCACACGT2369570.20484849927771748No Hit
AAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTA2339940.20228699612161796No Hit
AAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACCAGATCGGAAGAGCAC2233920.19312160413344137No Hit
TACCGGTTCGATTCCGGTCTTCGGCACCAGATCGGAAGAGCACACGTCTG2050660.17727884110992465Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGTATTAAGCTGACCGATACTAATAGGTCGAGGACTTGACCAGATCGG1981270.17128009983413164No Hit
AGACGAGCGGGAGAACCATTGTTAAGGAACTCAGATCGGAAGAGCACACG1970270.1703291536742567No Hit
CGCGGGGCAGAGCAGTTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGT1959870.16943007730492038No Hit
TCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTCGGAG1957820.16925285552058003No Hit
ATTGGTACAAGATGGGCCCGCGTCTGATTAGATCGGAAGAGCACACGTCT1954500.1689658426795996Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGGGGCTCAAACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1939040.16762933107672082Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCAGGGGGTCACAGGTTCAAGTCCTGTTGCGCCCACCAGATCGGAAGAG1930710.16690920548474278No Hit
TTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1921690.16612942963364533Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCCATTAGCTCAGTCGGTAGAGCACCTGAGATCGGAAGAGCACACGTC1911440.16524332071194364No Hit
ACCGGTAGGTTGGAGGTTCGAGTCCTCTCTCTGGAGCCAGATCGGAAGAG1848750.1598237920971654No Hit
AAGACGAGCGGGAGAACCATTGTTAAGGAACCAGATCGGAAGAGCACACG1802600.15583414070823548No Hit
AGAAAGACTATCTGGTAGATAGGTCGGAGGTGTAGATCGGAAGAGCACAC1787080.1544924421263028No Hit
ATGCGTGACCGCGTACTTTTTGTAGAACGGGCCAGATCGGAAGAGCACAC1762230.15234416830149441No Hit
ACCGGTAGGTTGGAGGTTCGAGTCCTCTCTCTGGAGCAGATCGGAAGAGC1747750.1510923773564954No Hit
TTTCAGGAGTCAGACTACAGATCGGAAGAGCACACGTCTGAACTCCAGTC1745870.15092985201280765Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGTCTCAGTTCGGATTGTGGGCTGAAACTAGATCGGAAGAGCACACGTC1698640.1468468350009082No Hit
AGGTCTCTGCAAAGTCGAAAGACGACGTATAGATCGGAAGAGCACACGTC1582330.13679187610499405No Hit
TTTCAGGAGTCAGACTACGAGATCGGAAGAGCACACGTCTGAACTCCAGT1548810.13389408380690235Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGGCGTAGCTCAGTTGGGAGAGCACCTGAGATCGGAAGAGCACACGTC1539730.13310912097675104No Hit
GACGGTACCTCGGGAGGAAGCCCCGGCAGATCGGAAGAGCACACGTCTGA1509430.13048969655455003Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGACGGTACCTCGGGAGGAAGCCCCGGCTGGGCGCATAGCTCAGCTGG1491460.12893619632791795No Hit
GCCGAAGCGGCGGAACTGGCAGACGCAGATCGGAAGAGCACACGTCTGAA1490250.1288315922503317Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACCGGTTCGATTCCGGTCTTCGGCAGATCGGAAGAGCACACGTCTGAAC1452550.12557244041148755Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTGTCGAGAAAAGCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1449200.12528283408098018Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGACGAGCGGGAGAACCATTGTTAAGGAACTCGGAGATCGGAAGAGCAC1447430.1251298181988912No Hit
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGAGATCGGAAGAGCACACGTC1392450.12037681639253438No Hit
ACGGCGGTAACAGGGGTTCGATTCCCCTAGGGGTCAGATCGGAAGAGCAC1380470.11934114957477966No Hit
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACAGATCGGAAGAGCA1376170.11896941607591946No Hit
AAGACGAGCGGGAGAACCATTAGATCGGAAGAGCACACGTCTGAACTCCA1354550.11710037462351068Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGACGAGCGGGAGAACCATTGTTAAGGAACTCGAGATCGGAAGAGCACA1342980.11610015216262402No Hit
AGAGTACCACGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1327920.11479822042010433Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACCTACCTTGTACAGGGGGATAACAGTCGGAGATCGGAAGAGCACACGT1326980.11471695774826046No Hit
AAGCTGACCGATACTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1314660.11365189804920053Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGGGGTAGAGCAGTTGGTAGCTCGTCAGGAGATCGGAAGAGCACACGT1313260.1135308685379437No Hit
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGTAGATCGGAAGAGCACACGT1312490.11346430230675245No Hit
GGGGGCGTAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA1306490.11294560440136613TruSeq Adapter, Index 4 (100% over 36bp)
AGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTCGGAGTTGCTAGT1301990.11255658097232638No Hit
CGGTCTCAGTTCGGATTGTGGGCTGAAACAGATCGGAAGAGCACACGTCT1281310.11076880219176145Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCAGGGGGTCAGGAGTTCGACTCTCCTCGTCTCCACCAGATCGGAAGAG1264730.10933546697987721No Hit
TAAGACGAGCGGGAGAACCATTGTTAAGGAACTAGATCGGAAGAGCACAC1258550.10880120813732928No Hit
AGGGGTGTAGTTCAGTTGGTAGAACGTCGGTCAGATCGGAAGAGCACACG1235330.10679384724348415No Hit
AAGTGAGTAAGACCCCAGAAAGACTATCTGGTAGATAGGTCGGAGGTGTA1234850.10675235141105324No Hit
AAAGTCGGTCTCAGTTCGGATTGTGGGCTGAGATCGGAAGAGCACACGTC1226190.10600369743427897No Hit
AAGCTGACCGATACTAATAGGTCGAGGACTTGACCAGATCGGAAGAGCAC1221540.10560170655760455No Hit
ATGCGTGACCGCGTACTTTTTGTAGAACGGGCAGATCGGAAGAGCACACG1189020.10279036391041059No Hit
ACGTGAAACCCTGTGGGAAGCAGATCGGAAGAGCACACGTCTGAACTCCA1176190.10168121488939282Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGACGAGCGGGAGAACCATTGTTAAGGAACCAGATCGGAAGAGCACACGT1173330.10143396888782533No Hit
ACACTTCGGTCGCAGAGAACAGGCCCAGATCGGAAGAGCACACGTCTGAA1166660.10085734971633752Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAAGAGAGAATGTTGGCATGAGTAGATCGGAAGAGCACACGTCTGAACT1165240.10073459121206275Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATGACGGTACCTCGGGAGGAAGCCCCGGAGATCGGAAGAGCACACGTCT1158460.10014846257897618Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)