FastQCFastQC Report
Mon 26 Jun 2023
ERR3804300.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3804300.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33242719
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGT14741944.434637250942078No Hit
AAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTC7381152.2203809501864153No Hit
AATGACGGTACCTCGGGAGGAAGCCCCGGCAGATCGGAAGAGCACACGTC4365381.3131837982326295No Hit
AAGACCCCAGAAAGACTATCTGGTAGATAGGTCGGAGGTGTAGATCGGAA4320941.2998154573336795No Hit
AAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTAGATCGGAAGAGCAC4008871.2059392614665485No Hit
AGCGTGAGGTCGGTGGTTCGAGTCCACTTGAGCCCAGATCGGAAGAGCAC3255420.9792881262209628No Hit
AAGACGAGCGGGAGAACCATTGTTAAGGAACTAGATCGGAAGAGCACACG3161540.9510473556630551No Hit
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAG3138670.9441676536747792No Hit
AATAGGTCGAGGACTTGACCAGATCGGAAGAGCACACGTCTGAACTCCAG2880990.8666529353390136Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA2503840.7531995201716202TruSeq Adapter, Index 4 (100% over 36bp)
ACGGCGGTAACAGGGGTTCGATTCCCCTAGGGGAGATCGGAAGAGCACAC2483000.7469304782199073No Hit
AGACGAGCGGGAGAACCATTGTTAAGGAACTAGATCGGAAGAGCACACGT1942890.5844558021863374No Hit
GGGGGCGTAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA1880480.5656817662839192TruSeq Adapter, Index 4 (100% over 36bp)
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTAGATCGGAAGAGC1695000.5098860896426674No Hit
AGAATGTCGCGGTGAATGCGAGATCGGAAGAGCACACGTCTGAACTCCAG1574420.4736134851063176Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGCAGATCGGAAGAGCACACGT1553140.4672120833437241No Hit
AGGCGAGCATTCTATGTGGGTTGAAGCTATACCGTAAGGAGTAGTGGACT1525610.45893057063112075No Hit
GCCGAAGTGGCGGAACTGGCAGACGCAGATCGGAAGAGCACACGTCTGAA1483790.44635037224241497Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTCGGTAGGCGAGCATTCTATGTGGGTTGAAGCTATACCGTAAGGAGTA1398820.4207898878548413No Hit
AATGACGGTACCTCGGGAGGAAGCCCCGGCTAGATCGGAAGAGCACACGT1395530.41980019745075603No Hit
AAGACCCCAGAAAGACTATCTGGTAGATAGGTCGGAGGTGCAGATCGGAA1195090.3595042872395606No Hit
ATAGGTCTCTGCAAAGTCGAAAGACGACGTAGATCGGAAGAGCACACGTC1176760.3539902978453718No Hit
AAAAACATAGGTCTCTGCAAAGTCGAAAGACGACGTAGATCGGAAGAGCA1132620.34071220227202237No Hit
AAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACAGATCGGAAGAGCACA1126820.33896745931041317No Hit
AGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTCGGAGTAGATCGG1100220.3309657071071713No Hit
AAGACGAGCGGGAGAACCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1096660.3298947959100457Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGGCAGATCGGAAGAGCACACG1020400.30695443414240575No Hit
ATTTTCAGGAGTCAGACTACGAGTAGATCGGAAGAGCACACGTCTGAACT995700.2995242356679669Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGAGGGAAAGAACCCAGATCAGATCGGAAGAGCACACGTCTGAACTCCA947350.28497969735869083Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGTGGAGGTCCGAACCAGTTGACGTTAGATCGGAAGAGCACACGTCTGA936960.2818542009153944Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGCGTAGCTCAGTTGGGAGAGCACCTGCAGATCGGAAGAGCACACGT913720.27486319635887785No Hit
AAGACCCCAGAAAGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA908550.27330796858102974Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGTTCGGATTGTGGGCTGAAACTAGATCGGAAGAGCACACGTCTGAACTC905030.2722490900939842Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCTACCCATGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG884940.26620566145627256TruSeq Adapter, Index 4 (100% over 35bp)
AAGACGAGCGGGAGAACCATTGTTAAGGAACTCAGATCGGAAGAGCACAC830360.2497870285520267No Hit
AAAGGGTGCGTAGGCGGCCAATAAAGTCTAGGGTGAAAGGCTACGGCTCA817700.24597867581168678No Hit
AGGTCTCTGCAAAGTCGAAAGACGACGTAGATCGGAAGAGCACACGTCTG791000.23794684183324472Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTA776590.2336120580269021No Hit
GGCGGCATAGCTCAGCTGGCTAGAGCGTTCGGAGATCGGAAGAGCACACG768650.23122356507600958No Hit
AAGCTGACCGATACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT744870.22407011893341217Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACCAGATCGGAAGAGCAC739550.2224697684927638No Hit
ATCAGAAGGTCGGGAGTTCGAATCTCTCTGGGCGCAGATCGGAAGAGCAC736310.2214951189762787No Hit
ACCGGTAGGTTGGAGGTTCGAGTCCTCTCTCTGGAGATCGGAAGAGCACA728710.21920890406106672No Hit
AGAGCAGTTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGTCTGAACTC715500.21523510155712594Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGACGAGCGGGAGAACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA700910.21084617055542298Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGGGGTAGAGCAGTTGGTAGCTCGTCCGGAGATCGGAAGAGCACACGT660220.19860589622647892No Hit
AATGTATTAAGCTGACCGATACTAATAGGTCGAGGACTTGACCAGATCGG584620.17586407417515998No Hit
TACCGGTTCGATTCCGGTCTTCGGCACCAGATCGGAAGAGCACACGTCTG577160.17361997374522822Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGGTACAAGATGGGCCCGCGTCTGATTAGATCGGAAGAGCACACGTCT572060.1720858032100202Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACGAGCGGGAGAACCATTGTTAAGGAACTCAGATCGGAAGAGCACACG558770.1680879352859193No Hit
AGCAGGGGGTCACAGGTTCAAGTCCTGTTGCGCCCACCAGATCGGAAGAG554780.16688767245543304No Hit
TTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC552680.1662559551762297Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGGTCTCAGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTCGGAG549620.1653354528551049No Hit
CGCGGGGCAGAGCAGTTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGT545000.16394567484085762No Hit
AAGACGAGCGGGAGAACCATTGTTAAGGAACCAGATCGGAAGAGCACACG544890.16391258488813745No Hit
TCCGGGGCTCAAACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC544660.1638433968051771Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCCATTAGCTCAGTCGGTAGAGCACCTGAGATCGGAAGAGCACACGTC520280.15650946001137875No Hit
ACCGGTAGGTTGGAGGTTCGAGTCCTCTCTCTGGAGCCAGATCGGAAGAG515940.1552039109676919No Hit
AGAAAGACTATCTGGTAGATAGGTCGGAGGTGTAGATCGGAAGAGCACAC509250.15319144020680137No Hit
ATGCGTGACCGCGTACTTTTTGTAGAACGGGCCAGATCGGAAGAGCACAC503080.15133539467695165No Hit
TTTCAGGAGTCAGACTACAGATCGGAAGAGCACACGTCTGAACTCCAGTC493810.14854681411589707Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCGGTAGGTTGGAGGTTCGAGTCCTCTCTCTGGAGCAGATCGGAAGAGC490120.14743679661101128No Hit
CGGTCTCAGTTCGGATTGTGGGCTGAAACTAGATCGGAAGAGCACACGTC481720.14490992749419807No Hit
AATGACGGTACCTCGGGAGGAAGCCCCGGCTGGGCGCATAGCTCAGCTGG459790.13831299419280355No Hit
AGGTCTCTGCAAAGTCGAAAGACGACGTATAGATCGGAAGAGCACACGTC454700.13678183183511552No Hit
TTTCAGGAGTCAGACTACGAGATCGGAAGAGCACACGTCTGAACTCCAGT437440.13158971743556835Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAGACGAGCGGGAGAACCATTGTTAAGGAACTCGGAGATCGGAAGAGCAC431860.1299111543793996No Hit
GACGGTACCTCGGGAGGAAGCCCCGGCAGATCGGAAGAGCACACGTCTGA423900.12751664507346705Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGTCGGTCTCAGTTCGGATTGTGGGCTGAGATCGGAAGAGCACACGTC421330.12674354345082303No Hit
ACTGTCGAGAAAAGCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC419220.1261088179940997Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGCGTAGCTCAGTTGGGAGAGCACCTGAGATCGGAAGAGCACACGTC416580.12531465912881554No Hit
ATAAAGTCGGTCTCAGTTCGGATTGTGGGCTGAAACAGATCGGAAGAGCA412690.12414447807352944No Hit
AAGACGAGCGGGAGAACCATTAGATCGGAAGAGCACACGTCTGAACTCCA411320.12373235775328727Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCGGTTCGATTCCGGTCTTCGGCAGATCGGAAGAGCACACGTCTGAAC408930.12301340332600351Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGACGAGCGGGAGAACCATTGTTAAGGAACTCGAGATCGGAAGAGCACA405350.12193647577383787No Hit
GCCGAAGCGGCGGAACTGGCAGACGCAGATCGGAAGAGCACACGTCTGAA403530.12138898746519501Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGCGGTAACAGGGGTTCGATTCCCCTAGGGGTCAGATCGGAAGAGCAC397490.11957204824310551No Hit
AGAGTACCACGGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT394360.11863048867934059Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGAGATCGGAAGAGCACACGTC390590.11749640575429464No Hit
AGTTCGGATTGTGGGCTGAAACTCGCCCACATGAAGTCGGAGTTGCTAGT390510.11747234033413452No Hit
AACCTACCTTGTACAGGGGGATAACAGTCGGAGATCGGAAGAGCACACGT378350.11381439646979538No Hit
AAGCTGACCGATACTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC377180.11346243969995355Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGTCTCAGTTCGGATTGTGGGCTGAAACAGATCGGAAGAGCACACGTCT366280.11018352620313639Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGGGGTAGAGCAGTTGGTAGCTCGTCGGTAGATCGGAAGAGCACACGT363400.10931717107737185No Hit
CGCGGGGTAGAGCAGTTGGTAGCTCGTCAGGAGATCGGAAGAGCACACGT362590.10907350869825058No Hit
AATGACGGTACCTCGGGAGGAAGCCCCGGAGATCGGAAGAGCACACGTCT358850.1079484503057647Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAGACGAGCGGGAGAACCATTGTTAAGGAACTAGATCGGAAGAGCACAC356210.10715429144048055No Hit
AGCAGGGGGTCAGGAGTTCGACTCTCCTCGTCTCCACCAGATCGGAAGAG353510.10634208351007629No Hit
GGGGGCGTAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA353270.1062698872495959TruSeq Adapter, Index 4 (100% over 36bp)
AAGTGAGTAAGACCCCAGAAAGACTATCTGGTAGATAGGTCGGAGGTGTA349280.10506962441910964No Hit
AAGCTGACCGATACTAATAGGTCGAGGACTTGACCAGATCGGAAGAGCAC347750.10460937325854724No Hit
AGGGGTGTAGTTCAGTTGGTAGAACGTCGGTCAGATCGGAAGAGCACACG340460.10241641184645575No Hit
AGACGAGCGGGAGAACCATTGTTAAGGAACCAGATCGGAAGAGCACACGT338080.101700465596692No Hit
AAGACCCCAGAAAGACTATCTGGTAGATAGGTCGGAGGTGGAGATCGGAA337930.10165534293389178No Hit
AAAAACATAGGTCTCTGCAAAGTCGAAAGACGACGTATAGATCGGAAGAG337360.10148387681525087No Hit
ACACTTCGGTCGCAGAGAACAGGCCCAGATCGGAAGAGCACACGTCTGAA334350.10057841538172614Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)