FastQCFastQC Report
Mon 26 Jun 2023
ERR3804301.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3804301.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128106986
Total Bases6.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTAACCGGCGGCCATGAAACTGGTCAGATCGGAAGAGCACACGTCTG107470148.389092847754611Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGACAGTATTGGGATTCTAACCGGCGGCCATGAAACTGGTCAGATCGGAA36480222.847637052361844No Hit
AAGAACCTTACCTAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA31427262.4532042304078563Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTAACCGGCGGCCATGAAACTGGTCAGATCGGAAGAGCACACGTCTGAA28559962.2293834935746597Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTAACCGGCGGCCATGAAACTGGTCAGATCGGAAGAGCACACGTCTGA24458491.9092237483442158Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCTAACCGGCGGCCATGAAACTGGCCAGATCGGAAGAGCACACGTCTG16396411.279899755037559Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGGGGTGGAGCAGTTGGTAGCTCGTCGGGCAGATCGGAAGAGCACACG12657790.9880639920761229No Hit
CGCAGAGGGTCAAGAGTTCGAGTCTCTTTATCTCCAGATCGGAAGAGCAC11831510.923564777333845No Hit
ACCGGTAGGTCCGGGGTTCGAGTCCCTGAAGGTCCAGATCGGAAGAGCAC11476060.8958184372552485No Hit
ACGATGAATACTAGGTGTAGGAGGAGATCGGAAGAGCACACGTCTGAACT11211730.8751849020942541Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGTGGTAACAGGAGTTCGAGTCTCCTAGGGGTCAGATCGGAAGAGCAC9784560.763780360893043No Hit
AGCGTGAGGTCGATGGTTCGAGTCCATTTAAGCCCAGATCGGAAGAGCAC7469820.5830923225373517No Hit
CGCGGGGTGGAGCAGTTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGT7367180.5750802692368393No Hit
ATTCTAACCGGCGGCCATGAAACTGGGCAGATCGGAAGAGCACACGTCTG7300780.5698971014742318Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGACAGTATTGGGATTCTAACCGGCGGCCATGAAACTGGCCAGATCGGAA5641000.4403350805552477No Hit
TAGGTCGCAGAGAATAGGCCCAAGCAACTGTTTAGCAAAAACACAGGTCT5420960.4231588119636192No Hit
GGTGGACGTAGTTCAGTTGGTAGAGCGCCAGATTGTGAGATCGGAAGAGC5211100.4067771916825832No Hit
CGATGAATACTAGGTGTAGGAGGAGATCGGAAGAGCACACGTCTGAACTC4983610.3890193779127705Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGAACCTTACCTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA4836210.37751336995782575Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTGGAGTGGCGGAACTGGCAGACGCACAGGACTTAAAATCCTGCGGGCA4773880.37264790539994436No Hit
ATTCTAACCGGCGGCCATGAAACTGGTAGATCGGAAGAGCACACGTCTGA4608730.3597563367855677Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCTGGGGTTGCGGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC4389800.34266671452250075Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCTAACCGGCGGCCATGAAACTGGCCAGATCGGAAGAGCACACGTCTGAA4328940.33791599780514703Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCAGAGGGTCACAGGTTCGAGCCCTGTTGTGCCCACCAGATCGGAAGAG4108490.32070772471377945No Hit
AGGGCGTAGGTCGGGTAACCGGCGCGAGGGTTCAAATCCCTCTTTCTCCG4084230.3188139950462967No Hit
AACGCATTGTACTGTAAACTGAGATCGGAAGAGCACACGTCTGAACTCCA3936360.30727129900628525Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTAAAACTCATCTCAGTTCGGATTGTAGGCTGAAACTCGCCTACATGAAG3867550.30190000723301696No Hit
CGCAGAGGGTCAAGAGTTCGAGTCTCTTTATCTCCACAGATCGGAAGAGC3817810.298017314996389No Hit
TTCCTGGGGTTGCGGATAGATCAGATCGGAAGAGCACACGTCTGAACTCC3741660.2920730646180373Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTAACCGGCGGCCATGAAACTGGCCAGATCGGAAGAGCACACGTCTGA3702940.28905059088658913Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGACCATCGGAAGAATTGTTGTCAAGGAACTCGAGATCGGAAGAGCACA3630140.2833678406890316No Hit
AGACCATCGGAAGAATTGTTGTCAAGGAACTAGATCGGAAGAGCACACGT3521920.274920213953047No Hit
AGCAGAGGGTCACAGGTTCGAGCCCTGTTGTGCCCAGATCGGAAGAGCAC3520310.2747945377467549No Hit
GGGCGCATAGCTCAGCTGGGAGAGCATCTGCAGATCGGAAGAGCACACGT3494100.2727485915561233No Hit
AGACCATCGGAAGAATTGTTGTCAAGGAACTCGAGATCGGAAGAGCACAC3419550.26692923678650904No Hit
AAGACCATCGGAAGAATTGTTGTCAAGGAACTAGATCGGAAGAGCACACG3233510.25240699988055293No Hit
AACGATGAATACTAGGTGTAGGAGGAGATCGGAAGAGCACACGTCTGAAC3146220.24559316382636617Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGACAGTATTGGGATTCTAACCGGCGGCCATGAAACTGGTCAGATCGGA3047700.2379027167183529No Hit
TTCCTGGGGTTGCGGATAGAGATCGGAAGAGCACACGTCTGAACTCCAGT3026650.23625955886589978Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCAGAGGGTCAAGAGTTCGAGTCTCTTTATCTCCACCAGATCGGAAGAG3008440.2348380907189558No Hit
ATTCTAACCGGCGGCCATGAAACTGGTCAGATCGGAAGAGCACACGTCTA2749580.21463154242033294Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGCTGGATAGCTCAGTCGGTAGAGCAGAGGACAGATCGGAAGAGCACACG2697770.21058726648990087No Hit
ACAAGCAGCGGAGCATGTGGTTTAATTCGAGATCGGAAGAGCACACGTCT2669810.20840471572721259Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGTATAGCTCAGTTGGGAGAGCATCTGCAGATCGGAAGAGCACACGT2639370.20602857677098108No Hit
AACATGTTCAGCTGACTGATACTAATAGGTCGAGGGCTTGACCAGATCGG2614850.20411455156707844No Hit
ATTGGATGTGTGTCTAAGCATTTAGACAGATCAGACAGGCAAATCCGTTT2593920.20248076088528066No Hit
TGACAGTATTGGGATTCTAACCGGCGGCCATGAAACTGGGCAGATCGGAA2585600.20183130371984556No Hit
ATAGCTCAGCTGGGAGAGCATCTGCAGATCGGAAGAGCACACGTCTGAAC2470130.19281774375676905Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCTGGATAGCTCAGTCGGTAGAGCAGAGGAGATCGGAAGAGCACACGTC2335670.18232182903748903No Hit
CGCGGGGTGGAGCAGTTGGTAGCTCGTCGGCCAGATCGGAAGAGCACACG2335650.1823202678423798No Hit
ATGGACTGACCTCTGGTGTACCAGTAGATCGGAAGAGCACACGTCTGAAC2335090.18227655437932166Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGGACGTAGTTCAGTTGGTAGAGCGCCAGATTGAGATCGGAAGAGCAC2319210.1810369654626017No Hit
ACCGGTAGGTCCGGGGTTCGAGTCCCTGAAGGTCCACAGATCGGAAGAGC2315660.18075985333071531No Hit
TATTGGATGTGTGTCTAAGCATTTAGACAGATCAGACAGGCAAATCCGTT2144010.16736089630584236No Hit
GGGGGTATAGCTCAGCTGGGAGAGCATCTGCAGATCGGAAGAGCACACGT2075030.1619763343741457No Hit
TAGGGGCTGACGCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2068020.16142913548836438Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGTGGTAACAGGAGTTCGAGTCTCCTAGGGGTCAGATCGGAAGAGCACA2059620.16077343354249238No Hit
CGCAGAGGGTCAAGAGTTCGAGTCTCTTTATCTCCAAGATCGGAAGAGCA2023390.15794532860214197No Hit
TTAAAACTCATCTCAGTTCGGATTGTAGGCTGAGATCGGAAGAGCACACG2005350.15653713061362634No Hit
TCTAACCGGCGGCCATGAAACTGGGCAGATCGGAAGAGCACACGTCTGAA1991630.15546615076870202Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGTCTCGACAACAAATCCGGCGAAATTGTAGTGCAAGTGAAGATGCTT1926830.15040787861483215No Hit
ATTCTAACCGGCGGCCATGAAACGGGTCAGATCGGAAGAGCACACGTCTG1903150.14855942360551672Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGTGGTAACAGGAGTTCGAGTCTCCTAGGGGCCAGATCGGAAGAGCAC1899270.14825655175432823No Hit
AGCTCAGCTGGGAGAGCATCTGCAGATCGGAAGAGCACACGTCTGAACTC1845130.1440303965936721Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGCGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA1821910.14221784907186874TruSeq Adapter, Index 8 (100% over 39bp)
AGACCATCGGAAGAATTGTTGTCAAGGAACTCGGAGATCGGAAGAGCACA1786850.13948107404540763No Hit
AAGACCATCGGAAGAATTGTTGTCAAGGAACTCGGAGATCGGAAGAGCAC1740420.1358567595993555No Hit
ACGGTGGTAACAGGAGTTCGAGTCTCCTAGGGGAGATCGGAAGAGCACAC1736610.13555935193104926No Hit
TTCTAACCGGCGGCCATGAAACTGGGCAGATCGGAAGAGCACACGTCTGA1704770.13307392931717243Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCTAACCGGCGGCCATGAAACTGGACAGATCGGAAGAGCACACGTCTG1665350.12999681375690159Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCAGAGGGTCAAGAGTTCGAGTCTCTTTATCTCCAGATCGGAAGAGCACA1636240.1277244942754332No Hit
AAGACCATCGGAAGAATTGTTGTCAAGGAACTCAGATCGGAAGAGCACAC1506370.11758687383371896No Hit
AGACCATCGGAAGAATTGTTGTCAAGGAACTCAGATCGGAAGAGCACACG1499110.11702016000907241No Hit
TCAGCTGGGAGAGCATCTGCAGATCGGAAGAGCACACGTCTGAACTCCAG1479210.11546677087539942Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCCGAAGGTCGTAGGTTCAAGTCCTACCTCCGCAGATCGGAAGAGCACA1468340.11461826133353882No Hit
AACTGTTTAGCAAAAACACAGGTCTCTGCTAAAGCGAAAGCTGAAGTATA1426510.11135302176260707No Hit
ACATGTTCAGCTGACTGATACTAATAGGTCGAGGGCTTGACCAGATCGGA1378640.10761630126869116No Hit
AACCGGCGGCCATGAAACTGGTCAGATCGGAAGAGCACACGTCTGAACTC1335110.10421836011347578Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGGGGCGGAGCAGTTGGTAGCTCGTCGGGCAGATCGGAAGAGCACACG1322560.1032387101824408No Hit
ACGCAGAGGGTCAAGAGTTCGAGTCTCTTTATCTCCAGATCGGAAGAGCA1306090.10195306600999886No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)