FastQCFastQC Report
Mon 26 Jun 2023
ERR3804302.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3804302.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151475714
Total Bases7.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTAACCGGCGGCCATGAAACTGGTCAGATCGGAAGAGCACACGTCTG91339096.029949461073344Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATGAATACTAGGTGTAGGAGGAGATCGGAAGAGCACACGTCTGAACT44573142.942593160511526Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTAACCGGCGGCCATGAAACTGGTCAGATCGGAAGAGCACACGTCTGAA32508142.1460958421361194Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGATGAATACTAGGTGTAGGAGGAGATCGGAAGAGCACACGTCTGAAC23746411.5676711053496009Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGGTAGGTCCGGGGTTCGAGTCCCTGAAGGTCCAGATCGGAAGAGCAC23040851.521092021391627No Hit
AAGAACCTTACCTAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA22248501.4687833060816602Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGGGGTGGAGCAGTTGGTAGCTCGTCGGGCAGATCGGAAGAGCACACG20675331.3649270535869533No Hit
TTCTAACCGGCGGCCATGAAACTGGTCAGATCGGAAGAGCACACGTCTGA17880801.1804400539085758Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGATGAATACTAGGTGTAGGAGGAGATCGGAAGAGCACACGTCTGAACTC15678611.0350576726774827Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGGGGTGGAGCAGTTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGT15490981.0226708685459638No Hit
GGGGGTATAGCTCAGCTGGGAGAGCATCTGCAGATCGGAAGAGCACACGT15463421.0208514349699649No Hit
GGGGGTATAGCTCAGTTGGGAGAGCATCTGCAGATCGGAAGAGCACACGT13633510.9000459307952164No Hit
TGACAGTATTGGGATTCTAACCGGCGGCCATGAAACTGGTCAGATCGGAA11067800.7306649830348382No Hit
TTAAAACTCATCTCAGTTCGGATTGTAGGCTGAAACTCGCCTACATGAAG10682100.7052021553765377No Hit
ACGGTGGTAACAGGAGTTCGAGTCTCCTAGGGGTCAGATCGGAAGAGCAC9986020.6592489143177104No Hit
ATTCTAACCGGCGGCCATGAAACTGGTAGATCGGAAGAGCACACGTCTGA8141660.5374894618420482Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG6640920.4384148339449319TruSeq Adapter, Index 9 (100% over 49bp)
ACAAGCAGCGGAGCATGTGGTTTAATTCGAGATCGGAAGAGCACACGTCT5174890.34163166248551236Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTGGAGGTCACCGGTTCAATCCCGGTCATGGGCAGATCGGAAGAGCAC5044380.3330157598728995No Hit
CGCGGGGCGGAGCAGTTGGTAGCTCGTCGGGCAGATCGGAAGAGCACACG4826550.31863523680106237No Hit
GGTGGACGTAGTTCAGTTGGTAGAGCGCCAGATTGTGAGATCGGAAGAGC4771310.3149884475870502No Hit
TTCCTGGGGTTGCGGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC4705940.31067290430464645Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGACCATCGGAAGAATTGTTGTCAAGGAACTAGATCGGAAGAGCACACG4693230.3098338259029431No Hit
AGGGCGTAGGTCGGGTAACCGGCGCGAGGGTTCAAATCCCTCTTTCTCCG4686790.3094086752414978No Hit
AGCGTGAGGTCGATGGTTCGAGTCCATTTAAGCCCAGATCGGAAGAGCAC4652670.3071561689420391No Hit
TCAAGCTACAAAGGGCGCATGGTGAATGCCTTGGCACCAGAAGCCGAAGA4580350.30238180623462846No Hit
AGACCATCGGAAGAATTGTTGTCAAGGAACTAGATCGGAAGAGCACACGT4254130.2808456806481863No Hit
ATTCTAACCGGCGGCCATGAAACTGGTCAGATCGGAAGAGCACACGTCTA3954890.2610906986713395Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TAGGTCGCAGAGAATAGGCCCAAGCAACTGTTTAGCAAAAACACAGGTCT3899630.25744258911365814No Hit
AAACGATGAATACTAGGTGTAGGAGGAGATCGGAAGAGCACACGTCTGAA3801870.2509887492591717Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTAACCGGCGGCCATGAAACTGGTAGATCGGAAGAGCACACGTCTGAAC3687030.24340733591128677Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGGGATGGACTGACCTCTGGTGTACCAGTAGATCGGAAGAGCACACGT3665270.2419708019993225No Hit
ACCGGTAGGTCCGGGGTTCGAGTCCCTGAAGGTCTAGATCGGAAGAGCAC3456500.2281883946095808No Hit
ATTCTAACCGGCGGCCATGAAACTGGTTAGATCGGAAGAGCACACGTCTG3439160.22704365664848428Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATGAATACTAGGTGTAGGAAGATCGGAAGAGCACACGTCTGAACTCC3289900.21718993184610436Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGGGGCTAAAACTTACCACCGAAGCTATGGGCAGATCGGAAGAGCACA3243120.21410164800411505No Hit
ACGGGGGCCCGCACAAGCAGCGGAGCATGTGGTTTAATTCGAGATCGGAA3072210.20281865117995085No Hit
GATGAATACTAGGTGTAGGAGGAGATCGGAAGAGCACACGTCTGAACTCC3056800.201801326382921Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTCTAACCGGCGGCCATGAAACTGGCCAGATCGGAAGAGCACACGTCTG3033300.2002499225717464Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAGCGGTAGAGGAGCTTCCTGTATGGAGTGAAGTCGTACCGAAAGGAG3031870.2001555179994068No Hit
CGCGGGGTGGAGCAGTTGGTAGCTCGTCGGGTAGATCGGAAGAGCACACG2944880.19441268321072247No Hit
ATGGACTGACCTCTGGTGTACCAGTAGATCGGAAGAGCACACGTCTGAAC2936300.1938462557766851Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGCACTGTCTCGACAACAAATCCGGCGAAATTGTAGTGCAAGTGAAGA2925440.19312930916437204No Hit
AGTAAGACCCCTTGAAGAACACAAGGTTGATAGGTCAGAGGTGTAAGCAT2829110.1867698738822251No Hit
AACGCATTGTACTGTAAACTGAGATCGGAAGAGCACACGTCTGAACTCCA2737290.18070817609745676Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGACCATCGGAAGAATTGTTGTCAAGGAACTCGAGATCGGAAGAGCACA2666250.1760183153848676No Hit
GCTGGAGTGGCGGAACTGGCAGACGCACAGGACTTAAAATCCTGCGGGCA2580680.17036922499668825No Hit
TTAAAACTCATCTCAGTTCGGATTGTAGGCTGAGATCGGAAGAGCACACG2438650.16099280443068253No Hit
TTCCGTGGAGGTCCGAACCACGTTGGTGTTGAAAAACCATGGGATGAGCT2407700.1589495726027738No Hit
GGGCGCTCAGCTGGGAGAGCATCTGCAGATCGGAAGAGCACACGTCTGAA2309820.1524878106862728Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCGACAACAAATCCGGCGAAATTGTAGTGCAAGTGAAGATGCTTGCTA2232220.14736487725022374No Hit
TTCTAACCGGCGGCCATGAAACTGGTAGATCGGAAGAGCACACGTCTGAA2182180.14406137739017358Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA2128140.14049380879630644TruSeq Adapter, Index 9 (100% over 40bp)
AAGACCATCGGAAGAATTGTTGTCAAGGAACTCGGAGATCGGAAGAGCAC2109710.13927711210524482No Hit
ACTGTCTCGACAACAAATCCGGCGAAATTGTAGTGCAAGTGAAGATGCTT2106830.13908698261689662No Hit
AACGATGAATACTAGGTGTAGGAAGATCGGAAGAGCACACGTCTGAACTC2099240.1385859121944789Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTAAAACTCATCTCAGTTCGGATTGTAGGCTGAAACTCGCCTACATGAA2081130.13739034100212263No Hit
GGTGGACGTAGTTCAGTTGGTAGAGCGCCAGATTGAGATCGGAAGAGCAC1985920.13110484496544444No Hit
GGGCGCATAGCTCAGCTGGGAGAGCATCTGCAGATCGGAAGAGCACACGT1968880.12997991215938418No Hit
CGCAGAGGGTCAAGAGTTCGAGTCTCTTTATCTCCAGATCGGAAGAGCAC1935840.12779870441805607No Hit
TTAAAACTCATCTCAGTTCGGATTGTAGGCTAGATCGGAAGAGCACACGT1917490.12658728910167077No Hit
CGCGGGGCGGAGCAGTTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGT1865470.12315307521838122No Hit
AACATGTTCAGCTGACTGATACTAATAGGTCGAGGGCTTGACCAGATCGG1831500.12091047149644069No Hit
AAGAACCTTACCTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1813570.11972678339710616Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGATGAATACTAGGTGTAGGAGAAGATCGGAAGAGCACACGTCTGAACT1807990.11935840751343149Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGGTGGTAACAGGAGTTCGAGTCTCCTAGGGGAGATCGGAAGAGCACAC1798500.11873190444245076No Hit
ACGGTGGTAACAGGAGTTCGAGTCTCCTAGGGGTAGATCGGAAGAGCACA1726880.11400375376345807No Hit
AGACCATCGGAAGAATTGTTGTCAAGGAACTCGGAGATCGGAAGAGCACA1688430.11146539305964256No Hit
GGGGGTATAGCTCAGCTGGGAGAGCATCTGTAGATCGGAAGAGCACACGT1683970.11117095642143664No Hit
GGCTGGATAGCTCAGTCGGTAGAGCAGAGGACAGATCGGAAGAGCACACG1661970.10971857838544335No Hit
AGACCATCGGAAGAATTGTTGTCAAGGAACTCGAGATCGGAAGAGCACAC1649750.10891184840363254No Hit
ACGATGAATACTAGGTGTAGGAGGAGATCGGAAGAGCACACGTCTAAACT1642490.10843256365175476Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AAGCTAGTAAGACCCCTTGAAGAACACAAGGTTGATAGGTCAGAGGTGTA1635940.10800015110012948No Hit
TAAACGATGAATACTAGGTGTAGGAGGAGATCGGAAGAGCACACGTCTGA1602010.10576018806552712Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGCTCAGTTGGGAGAGCATCTGCAGATCGGAAGAGCACACGTCTGAA1563870.1032422926885824Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGGATGTGTGTCTAAGCATTTAGACAGATCAGACAGGCAAATCCGTTT1540310.10168692784640052No Hit
GGCTGGATAGCTCAGTCGGTAGAGCAGAGGAGATCGGAAGAGCACACGTC1519540.10031575094605594No Hit
TCCGGGGCTAAAACTTACCACCGAAGCTATGGGCTCGAAAGAGCGGAGAT1519160.10029066441634334No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)