FastQCFastQC Report
Thu 29 Jun 2023
ERR3804304.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3804304.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127842081
Total Bases6.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTAACCGGCGGCCATGAAACTGGTCAGATCGGAAGAGCACACGTCTGAA72708915.687400379535436Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTCTAACCGGCGGCCATGAAACTGGTCAGATCGGAAGAGCACACGTCTG71260345.574091053790027Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCAGAGGGTCAAGAGTTCGAGTCTCTTTATCTCCAGATCGGAAGAGCAC55503614.341575916618566No Hit
ACCGGTAGGTCCGGGGTTCGAGTCCCTGAAGGTCCAGATCGGAAGAGCAC35614132.7857908539520726No Hit
TTCTAACCGGCGGCCATGAAACTGGTCAGATCGGAAGAGCACACGTCTGA30111582.3553731106739413Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGTGAGGTCGATGGTTCGAGTCCATTTAAGCCCAGATCGGAAGAGCAC28666252.2423172226052857No Hit
ACGATGAATACTAGGTGTAGGAGGAGATCGGAAGAGCACACGTCTGAACT18617371.4562787037235414Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGAACCTTACCTAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA12897651.0088735961674467Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTAACCGGCGGCCATGAAACTGGCCAGATCGGAAGAGCACACGTCTGAA11591680.9067186570594075Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTCTAACCGGCGGCCATGAAACTGGCCAGATCGGAAGAGCACACGTCTG11394310.8912800785838272Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGGACGTAGTTCAGTTGGTAGAGCGCCAGATTGTGAGATCGGAAGAGC11144830.8717653774737913No Hit
AGCAGAGGGTCACAGGTTCGAGCCCTGTTGTGCCCAGATCGGAAGAGCAC9998060.7820633019889593No Hit
CGCGGGGTGGAGCAGTTGGTAGCTCGTCGGGAGATCGGAAGAGCACACGT9278010.7257399071906535No Hit
CGATGAATACTAGGTGTAGGAGGAGATCGGAAGAGCACACGTCTGAACTC9045890.7075831314103843Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCAGAGGGTCAAGAGTTCGAGTCTCTTTATCTCCAGATCGGAAGAGCACA8936700.6990421252607739No Hit
ACAAGCAGCGGAGCATGTGGTTTAATTCGAGATCGGAAGAGCACACGTCT8896830.6959234338496102Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCTGGGGTTGCGGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC5726740.4479542225223947Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACGATGAATACTAGGTGTAGGAGGAGATCGGAAGAGCACACGTCTGAAC5567000.4354591192863952Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTAACCGGCGGCCATGAAACTGGGCAGATCGGAAGAGCACACGTCTGAA5437540.4253325632269706Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTCTAACCGGCGGCCATGAAACTGGGCAGATCGGAAGAGCACACGTCTG5317490.41594207153120416Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCAGAGGGTCAAGAGTTCGAGTCTCTTTATCTCCAGATCGGAAGAGCA4992530.3905232112108688No Hit
TTCTAACCGGCGGCCATGAAACTGGCCAGATCGGAAGAGCACACGTCTGA4824370.37736948290133043Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGAACCTTACCTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG4674170.3656206128246614Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGTGAGGTCGATGGTTCGAGTCCATTTAAGCCCAGATCGGAAGAGCACA4280970.3348639169914639No Hit
GGTGGACGTAGTTCAGTTGGTAGAGCGCCAGATTGAGATCGGAAGAGCAC3864780.3023089087543874No Hit
TTAAAACTCATCTCAGTTCGGATTGTAGGCTGAAACTCGCCTACATGAAG3844680.30073665650045234No Hit
AGCTCAGCTGGGAGAGCATCTGCAGATCGGAAGAGCACACGTCTGAACTC3824290.29914172001001765Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGCGTAGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG3802400.2974294512618267Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTGTCTCGACAACAAATCCGGCGAAATTGTAGTGCAAGTGAAGATGCTT3739550.2925132296618357No Hit
AGCATGTCGCGGTGAATACGAGATCGGAAGAGCACACGTCTGAACTCCAG3621740.2832979541376521Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACCGGCGGCCATGAAACTGGTCAGATCGGAAGAGCACACGTCTGAACTC3589960.2808120747033209Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCGGTAGGTCCGGGGTTCGAGTCCCTGAAGGCCCAGATCGGAAGAGCAC3424980.26790709078022595No Hit
ATGTCGCGGTGAATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3343160.261507007227143Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGGTAGGTCCGGGGTTCGAGTCCCTGAAGGTCCAGATCGGAAGAGCACA3129210.24477151619582915No Hit
AGGGCGTAGGTCGGGTAACCGGCGCGAGGGTTCAAATCCCTCTTTCTCCG3081620.24104895476474603No Hit
TCCGGGGCTAAAACTTACCACCGAAGCTATGGGCAGATCGGAAGAGCACA3063530.23963392773620448No Hit
ATGGACTGACCTCTGGTGTACCAGTAGATCGGAAGAGCACACGTCTGAAC3021540.23634940673407842Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGACCATCGGAAGAATTGTTGTCAAGGAACTAGATCGGAAGAGCACACG2924610.22876739623786316No Hit
CGCGGGGTGGAGCAGTTGGTAGCTCGTCGGGCAGATCGGAAGAGCACACG2772560.21687381637662795No Hit
AGACCATCGGAAGAATTGTTGTCAAGGAACTAGATCGGAAGAGCACACGT2747850.21494096298385504No Hit
ACGGTGGTAACAGGAGTTCGAGTCTCCTAGGGGAGATCGGAAGAGCACAC2716050.21245351911942048No Hit
GGGCGCATAGCTCAGCTGGGAGAGCATCTGCAGATCGGAAGAGCACACGT2611180.2042504298721483No Hit
AATAGGTCGAGGGCTTGACCAGATCGGAAGAGCACACGTCTGAACTCCAG2562610.20045121136599772Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGTGAGGTCGATGGTTCGAGTCCATTTAAGCCCAGATCGGAAGAGCACAC2426000.18976537154460119No Hit
ATAGCTCAGCTGGGAGAGCATCTGCAGATCGGAAGAGCACACGTCTGAAC2408310.18838163311812797Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATGAATACTAGGTGTAGGAGGAGATCGGAAGAGCACACGTCTGAACTCC2283840.17864540236950618Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACAGTATTGGGATTCTAACCGGCGGCCATGAAACTGGTCAGATCGGAA2259250.17672193555735377No Hit
TTCTAACCGGCGGCCATGAAACTGGGCAGATCGGAAGAGCACACGTCTGA2257580.17659130564371836Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCAGAGGGTCAAGAGTTCGAGTCTCTTTATCCCCAGATCGGAAGAGCAC2238110.17506833293804094No Hit
ATTCTAACCGGCGGCCATGAAACTGGTAGATCGGAAGAGCACACGTCTGA2160130.16896861996481424Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGACCATCGGAAGAATTGTTGTCAAGGAACTCGAGATCGGAAGAGCACA2116490.1655550334791562No Hit
ACGGTGGTAACAGGAGTTCGAGTCTCCTAGGGGTCAGATCGGAAGAGCAC2084770.1630738473351353No Hit
CAGCTGACTGATACTAATAGGTCGAGGGCTTGACCAGATCGGAAGAGCAC1966790.15384527415507263No Hit
TCAGCTGACTGATACTAATAGGTCGAGGGCTTGACCAGATCGGAAGAGCA1804800.14117417253243866No Hit
GGGGGTATAGCTCAGCTGGGAGAGCATCTGCAGATCGGAAGAGCACACGT1712210.13393164336866512No Hit
GGGGGTATAGCTCAGTTGGGAGAGCATCTGCAGATCGGAAGAGCACACGT1680600.131459061590213No Hit
AAGAACCTTACCTAGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC1648660.1289606667150545Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCTCGACAACAAATCCGGCGAAATTGTAGTGCAAGTGAAGATGCTTGCTA1643350.12854531052259702No Hit
ATTCTAACCGGCGGCCATGAAACTGGTCAGATCGGAAGAGCACACGTCTA1628680.12739780104173992Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AGCTGACTGATACTAATAGGTCGAGGGCTTGACCAGATCGGAAGAGCACA1620040.12672196723706336No Hit
AGACCATCGGAAGAATTGTTGTCAAGGAACTCGAGATCGGAAGAGCACAC1612590.12613921702354017No Hit
AACATGTTCAGCTGACTGATACTAATAGGTCGAGGGCTTGACCAGATCGG1564410.12237050490440625No Hit
TCTAACCGGCGGCCATGAAACTGGTAGATCGGAAGAGCACACGTCTGAAC1548370.12111583196146503Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTAAAACTCATCTCAGTTCGGATTGTAGGCTGAGATCGGAAGAGCACACG1540860.12052838845763157No Hit
ATGGACTGACCTCTGGTGTACCAGAGATCGGAAGAGCACACGTCTGAACT1530170.11969220056735465Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCAGAGGGTCACAGGTTCGAGCCCTGTTGTGCCCAGATCGGAAGAGCAC1529060.11960537469661496No Hit
AAGACCATCGGAAGAATTGTTGTCAAGGAACAGATCGGAAGAGCACACGT1527550.11948726022380689No Hit
AGACCATCGGAAGAATTGTTGTCAAGGAACAGATCGGAAGAGCACACGTC1523100.1191391745257964No Hit
TCGCGGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA1491820.11669240584405068TruSeq Adapter, Index 11 (100% over 40bp)
ACAGCTCAGCTGGGAGAGCATCTGCAGATCGGAAGAGCACACGTCTGAAC1373800.10746070380378116Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGGACGTAGTTCAGTTGGTAGAGCGCCAGATTGGGAGATCGGAAGAGC1349410.10555288129266295No Hit
CGGTAGGTCCGGGGTTCGAGTCCCTGAAGGTCCAGATCGGAAGAGCACAC1326630.10377099540486985No Hit
ACGATGAATACTAGGTGTAGGGGGAGATCGGAAGAGCACACGTCTGAACT1325400.10367478295350964Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)