FastQCFastQC Report
Mon 26 Jun 2023
ERR391719.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR391719.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28439705
Total Bases811.5 Mbp
Sequences flagged as poor quality0
Sequence length20-94
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGGAATATCCTCAGCACGTTTCTT296059210.410065786547364No Hit
TTAGCCACGGAGCCTACGCGCCTCCTCATG10658593.747785006911992No Hit
TTTTACACCCGTGGCGTGCGCAATT10305083.6234834362733372No Hit
TTTTACACCCGTGGCGTGCGCAAT7342522.5817848673184196No Hit
TCAGATACAAGAAACACGGGAGCGGTTCCTCCT6129082.155113774914332No Hit
TTAGCCACAGAGCCTACGCGCCTCCTCATG4813691.6925949126406197No Hit
TTAGCCACGGAGCCTACGCGCCTCCTC4093681.4394242134368131No Hit
TACGGGAATATCCTCAGCACGTTTCTT3941781.3860129702470543No Hit
TTAGCCACGGAGCCTACGCGCCTCCT3819831.3431327786276264No Hit
ACGGAGCCTACGCGCCTCCTCATG3521101.238093011161684No Hit
TTTACACCCGTGGCGTGCGCAATT3264551.1478846211660776No Hit
TTACGGGAATATCCTCAGCACGTTTCTT2652260.9325905455067132No Hit
ACGTCCAGCGAATGAATGAAATTAGAACCAACG2631530.9253014403630417No Hit
TTAGCCACGGAGCCTACGCGCCTCCTCA2279060.8013655556553768No Hit
TCAGATACAAGAAACACGGGAGCGGTTCCT2135360.7508376053830376No Hit
TTTACGGGAATATCCTCAGCACGTTTCTT1962280.6899790275602367No Hit
TTCCCCAACGGTGGTCGTCATCCTTCTT1918790.6746870264652886No Hit
TTAGCCACGGAGCCTACGCGCCTCCTCAT1816860.6388462890174142No Hit
TCAGATACAAGAAACACGGGAGCGGTTCCTCC1679470.590537067807138No Hit
ACAGAGCCTACGCGCCTCCTCATG1654700.5818274134700061No Hit
TTGCGCAGTCTTCGGGCTGTGCGCCGTCTAGGCTGGGCA1502290.5282368435256273No Hit
TTAGCCACAGAGCCTACGCGCCTCCTCAT1344380.4727123576000525No Hit
ACGTCCCGAGGCGCTGAACCTTGATGCTTGG1280180.4501382837831827No Hit
ACGGAGCCTACGCGCCTCCTC1222620.4298989739872478No Hit
TAGCCACGGAGCCTACGCGCCTCCTCATG1134750.39900202902948534No Hit
ACGGGAATATCCTCAGCACGTTTCT1134660.3989703831316112No Hit
TAGCCACAGAGCCTACGCGCCTCCTCATG1072700.3771839405507195No Hit
ACGGAGCCTACGCGCCTCCT1072460.37709955148972185No Hit
CAGATACAAGAAACACGGGAGCGGTTCCTCCT941690.3311180618786306No Hit
ACACCCGTGGCGTGCGCAATT924870.32520379518704573No Hit
AAGAAACACGGGAGCGGTTCCTCC920660.3237234704087121No Hit
TTAGCCACAGAGCCTACGCGCCTCCT902700.31740835567738834No Hit
TTTTACGGGAATATCCTCAGCACGTTTCTT885260.3112760839115596No Hit
TTACACCCGTGGCGTGCGCAATT876870.3083259829875169No Hit
TTTACGTCCCGAGGCGCTGAACCTTGATGCTTGG868130.3052528146828527No Hit
TCAGATACAAGAAACACGGGAGCGGTTCC803900.28266819223335826No Hit
ACAGGACAGCAGTCCCTCCGGCGGGGA778180.27362449786311077No Hit
ACGGAGCCTACGCGCCTCCTCA771960.2714374146989218No Hit
TTGCGCAGTCTTCGGGCTGTGCGCCGTCTAGGCTGGGC764090.2686701567403741No Hit
TTTACACCCGTGGCGTGCGCAAT664510.23365572884810165No Hit
TTAGCCACAGAGCCTACGCGCCTCCTCA651010.22890884416698418No Hit
ACGGAGCCTACGCGCCTCCTCAT636100.22366617375250553No Hit
ACGTGCCTATTCTATCACCCCCGGT634810.22321258254964318No Hit
TTACACCCGTGGCGTGCGCAAT538970.18951321752458403No Hit
ACACCCGTGGCGTGCGCAAT515160.18114111943144276No Hit
ACGTCCCGAGGCGCTGAACCTTGATGCTTGGA514980.18107782763569455No Hit
TTAGCCACAGAGCCTACGCGCCTCCTC500550.17600393534321118No Hit
TTACGTGCCTATTCTATCACCCCCGGT498300.17521278789635827No Hit
TACACCCGTGGCGTGCGCAATT473190.16638358238947978No Hit
ACACAGGACAGCAGTCCCTCCGGCGGGGA459250.16148198442986664No Hit
TTACGTCCCGAGGCGCTGAACCTTGATGCTTGG411630.1447377882435841No Hit
AAGAAACACGGGAGCGGTTCCTCCT405470.1425718023446446No Hit
TAGCCACGGAGCCTACGCGCCTCCTC401550.14119344768168304No Hit
ACGGGAATATCCTCAGCACGTTTC385760.13564135071021305No Hit
TTTACGTCCCGAGGCGCTGAACCTTGATGCTTGGA380510.1337953400008896No Hit
TAGCCACGGAGCCTACGCGCCTCCT376590.1324169853379281No Hit
TTGCGCAGTCTTCGGGCTGTGCGCCGTCTAGGCTGGG341590.12011024727577167No Hit
TAGCCACAGAGCCTACGCGCCTCCTCAT322830.11351383567445583No Hit
TACACCCGTGGCGTGCGCAAT310990.10935064199857206No Hit
TTTGCGCAGTCTTCGGGCTGTGCGCCGTCTAGGCTGGGCA288470.10143213510829314No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)