FastQCFastQC Report
Mon 26 Jun 2023
ERR391720.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR391720.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15817255
Total Bases460.2 Mbp
Sequences flagged as poor quality0
Sequence length20-94
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTACACCCGTGGCGTGCGCAATT8281665.2358389619437755No Hit
ACGGGAATATCCTCAGCACGTTTCTT7017604.436673746487617No Hit
TTTTACACCCGTGGCGTGCGCAAT4589082.9013125223055454No Hit
TTAGCCACGGAGCCTACGCGCCTCCTCATG3262192.062424864491342No Hit
TTAGCCACAGAGCCTACGCGCCTCCTCATG2848281.8007422906186947No Hit
ACGTCCAGCGAATGAATGAAATTAGAACCAACG2583891.6335893933555474No Hit
TTAGCCACGGAGCCTACGCGCCTCCTC2529921.5994684286243093No Hit
TTTACACCCGTGGCGTGCGCAATT2132971.3485083220824348No Hit
TTAGCCACAGAGCCTACGCGCCTCCTCAT1690581.0688200955222635No Hit
TTAGCCACGGAGCCTACGCGCCTCCTCAT1337870.8458294438573569No Hit
TTAGCCACGGAGCCTACGCGCCTCCTCA1274160.8055506470623379No Hit
TACGGGAATATCCTCAGCACGTTTCTT1268140.8017446769366745No Hit
TCGTCCCGAGGCGCTGAACCTTGATGCTTGG1035590.6547216947567703No Hit
TTTACGGGAATATCCTCAGCACGTTTCTT1027680.649720827033515No Hit
TTAGCCACGGAGCCTACGCGCCTCCT813360.5142232327922892No Hit
ACGGAGCCTACGCGCCTCCTCATG812970.5139766666213575No Hit
TCGTCCCGAGGCGCTGAACCTTGATGCTTGGA746060.47167476278279635No Hit
TTACGGGAATATCCTCAGCACGTTTCTT731670.4625771032963685No Hit
ACAGAGCCTACGCGCCTCCTCATG705010.4457220927398591No Hit
ACGTCCCGAGGCGCTGAACCTTGATGCTTGGA699880.4424787992606808No Hit
ACGTCCCGAGGCGCTGAACCTTGATGCTTGG676290.42756470702406957No Hit
TAGCCACAGAGCCTACGCGCCTCCTCATG644700.40759284717860333No Hit
ACACCCGTGGCGTGCGCAATT611410.3865462117162555No Hit
TTACACCCGTGGCGTGCGCAATT582250.3681106487819789No Hit
TTGCGCAGTCTTCGGGCTGTGCGCCGTCTAGGCTGGGC559830.35393625505816273No Hit
TTAGCCACAGAGCCTACGCGCCTCCTCA552890.349548641657481No Hit
TCAGATACAAGAAACACGGGAGCGGTTCCT517450.32714273115025333No Hit
TCAGATACAAGAAACACGGGAGCGGTTCCTCCT504300.31882902564319787No Hit
ACGTCCAGCGAATGAATGAAATTAGAACCAACGC503750.3184813041200891No Hit
TTGCGCAGTCTTCGGGCTGTGCGCCGTCTAGGCTGGGCA497710.31466268957540355No Hit
ACGGAGCCTACGCGCCTCCTC490250.30994632128014626No Hit
TAGCCACAGAGCCTACGCGCCTCCTCAT409460.2588691906402217No Hit
TACACCCGTGGCGTGCGCAATT408620.25833812504129194No Hit
TTGCGCAGTCTTCGGGCTGTGCGCCGTCTAGGCTGGG391370.24743231363469834No Hit
TTAGCCACAGAGCCTACGCGCCTCCTC360360.22782714194087406No Hit
TAGCCACGGAGCCTACGCGCCTCCTCATG359760.22744780937020995No Hit
TTTACGTCCCGAGGCGCTGAACCTTGATGCTTGGA359200.2270937656375901No Hit
ACGGAGCCTACGCGCCTCCTCAT344390.21773057335169724No Hit
TTACACCCGTGGCGTGCGCAAT340450.21523962280433614No Hit
TTTTACGGGAATATCCTCAGCACGTTTCTT338880.21424703591109837No Hit
TTTACACCCGTGGCGTGCGCAAT321370.20317684705721695No Hit
TTGACCCACGCCCTCGTGGCCCGTGAACACA304390.19244173530742217No Hit
TCAGATACAAGAAACACGGGAGCGGTTCCTCC298690.18883807588611298No Hit
TTAGCCACAGAGCCTACGCGCCTCCT296020.18715004594665763No Hit
TTTACGTCCCGAGGCGCTGAACCTTGATGCTTGG289050.18274346591744267No Hit
ACGGAGCCTACGCGCCTCCTCA279760.1768701332816598No Hit
TAGCCACGGAGCCTACGCGCCTCCTC256150.16194339662602644No Hit
ACACCCGTGGCGTGCGCAAT242840.15352853576679393No Hit
ACGTTGCTTCACTTATCGAATGAAGAGACC225410.14250892458900105No Hit
TTACGTCCCGAGGCGCTGAACCTTGATGCTTGGA223590.14135828245798657No Hit
TCGCCCAGCCGTGCGGAACTACTGCGCCAACGGG212820.13454926281456547No Hit
TACACCCGTGGCGTGCGCAAT211870.13394865291101396No Hit
TTACGTCCCGAGGCGCTGAACCTTGATGCTTGG211400.1336515090639937No Hit
ACGGAGCCTACGCGCCTCCT194000.12265086451473406No Hit
TAGCCACGGAGCCTACGCGCCTCCTCAT159670.100946719263235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)