FastQCFastQC Report
Mon 26 Jun 2023
ERR391723.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR391723.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29273025
Total Bases930.3 Mbp
Sequences flagged as poor quality0
Sequence length20-94
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACGTGCCTATTCTATCACCCCCGGT3734901.2758845387519737No Hit
ACGGGAATATCCTCAGCACGTTTCTT3401641.1620391128009488No Hit
TTCCCCAACGGTGGTCGTCATCCTTCTT3389561.1579124466979411No Hit
TACGGGAATATCCTCAGCACGTTTCTT3175711.0848588418860026No Hit
TTTACGTGCCTATTCTATCACCCCCGGT1717720.5867927896074969No Hit
TTACGGGAATATCCTCAGCACGTTTCTT1593210.544258750163333No Hit
TGGCGCGCACATTCGGCTCTTCGTGATG1339270.4575099430277534No Hit
TTTACGGGAATATCCTCAGCACGTTTCTT1027180.3508964310999632No Hit
ACGTGCCTATTCTATCACCCCCGGT998490.34109559910531967No Hit
TTTACGTGCCTATTCTATCACCCCCGG990570.33839003656096356No Hit
CCAACGGTGGTCGTCATCCTTCTT786560.2686978882435279No Hit
GGCGCGCACATTCGGCTCTTCGTGATG650470.22220798841254022No Hit
TTACGTGCCTATTCTATCACCCCCGG635400.2170599041267515No Hit
TACGTGCCTATTCTATCACCCCCGGT582870.1991150555844502No Hit
TCTCCGCATTACTGGATAACTTGGCGA514500.17575908195343665No Hit
TTTTACGGGAATATCCTCAGCACGTTTCTT433570.14811246873187858No Hit
TCCCCAACGGTGGTCGTCATCCTTCTT408720.13962342463752891No Hit
CCCAACGGTGGTCGTCATCCTTCTT316330.10806194440103133No Hit
TCAGATACAAGAAACACGGGAGCGGTTCCTCCT315390.10774082965460523No Hit
TTTCCCCAACGGTGGTCGTCATCCTTCTT312620.10679456598694531No Hit

[OK]Adapter Content

Adapter graph