FastQCFastQC Report
Mon 26 Jun 2023
ERR3949729.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949729.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209920058
Total Bases6.1 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCC3971781118.92044589659936No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCC105451455.023409911595966No Hit
GGATGATCCTATGCTGTCGAGAAAAGCC87000734.144469605662933No Hit
GGGACGGACGAACCTCTGGTGTGCCAGT74788953.56273481974743No Hit
CGGGACGGACGAACCTCTGGTGTGCCAGT60578942.8858099877239933No Hit
ATTCGATACGGGCTAGCTAGAGTGTGG56750332.703425796500113No Hit
GTGGATGATCCTATGCTGTCGAGAAAAGCC50446882.403147201874344No Hit
ACCCTGTGGGAGAGTAGGACGCCGCCGA45211422.1537446412100363No Hit
ACAAGCAGCGGAGCATGTGGCTTAATTC30638321.459523224788743No Hit
AAACGGTGGGAACTAGGTGTTGGCG24519931.1680603670564915No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGA24038231.145113536506359No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAAC22661841.0795461956284331No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAAC20303860.9672186733103895No Hit
AACACGTGGGCAATCTGCCCTGCACTCTGGG16334350.7781224031483452No Hit
ACGTGGGCAATCTGCCCTGCACTCTGGG16315560.7772273004993167No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGA14840340.7069519769282838No Hit
CTTGGGCTGCACACGTGCTACAATGGCCGATACA14314670.68191053948737No Hit
ACCCTGTGGGAGAGTAGGACGTCGCCGA13180700.6278914042601874No Hit
AACACGTGGGCAATCTGCCCTGCACTCTGG12290740.5854962178030648No Hit
GTTGTCCGGAATTATTGGGCGTAAAGAGCTCG11424010.544207643082873No Hit
GGACGGACGAACCTCTGGTGTGCCAGT10556610.502887151450768No Hit
CACAAGCAGCGGAGCATGTGGCTTAATTC10217970.48675529615183316No Hit
CTAATACCGGATAATACTCTCGCAGG8931590.42547577802212694No Hit
AACACCGGTGGCGAAGGCGGATCTCTGGGCCAT8729170.41583305964978345No Hit
ATTCACGGAGAGTTTGATCCTGGCTCAGG8712320.4150303731337574No Hit
GGGGTGATGGGGACTCACAGGAGACCGCCGGGG8364150.3984445354907438No Hit
ACACCGGTGGCGAAGGCGGATCTCTGGGCCAT8134340.3874970347045159No Hit
ACCGGTGGCGAAGGCGGATCTCTGGGCCAT7874980.37514185519137005No Hit
ACCGCTGGAGGTGACTGGTACTAATAGGCCGAGGGCT7278240.34671484322855894No Hit
CACGTCGTCGGTGCCGCAGCTAACGCAT7159770.3410712662817576No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCC6954400.33128801822263215No Hit
CGGGACGGACGAACCTCTGGTGTGCCAG6755630.3218191755644427No Hit
GGGGTGATGGGGACTCACAGGAGACCGCCGGGGT6436390.3066114815955319No Hit
CCCTGTGGGAGAGTAGGACGCCGCCGA6396790.3047250491899159No Hit
ACGTGGGCAATCTGCCCTGCACTCTGGGC6234280.2969835307495961No Hit
GGGACGGACGAACCTCTGGTGTGCCAG6230960.2968253753054889No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGG5501670.26208405487387965No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAG5425990.25847887294314675No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACT5357200.2552019111961183No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGT5321510.25350174017196586No Hit
CGGGTGTAGTTTAATGGTAGAACATGAG5235730.24941542270343695No Hit
AAAAAGTCGGTCTCAGTTCGGATTGGGG5178190.24667437925345848No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGG5003740.2383640728605363No Hit
GGAGGTGACTGGTACTAATAGGCCGAGGGCT4534910.21603033284222892No Hit
TTCACGGAGAGTTTGATCCTGGCTCAGG4445810.2117858599295928No Hit
TGGATGATCCTATGCTGTCGAGAAAAG4390240.20913866172807555No Hit
TGGATGATCCTATGCTGTCGAGAAAAGC4360890.20774051043754954No Hit
GGGGTGATGGGGACTCACAGGAGACCGCCGGG4023020.19164533576872392No Hit
AACACGTGGGCAATCTGCCCTGCACTCTGGGC3706230.17655435289561516No Hit
GATGATCCTATGCTGTCGAGAAAAGCC3684780.17553253534257313No Hit
ATTGGGCGTAAAGAGCTCGTAGGCGGCTTGT3644490.17361323328140468No Hit
ACCGGTGGCGAAGGCGGATCTCTGGGC3629630.17290534475747904No Hit
GGGACGGACGAACCTCTGGTGTGC3619910.17244231134882784No Hit
AACACCGGTGGCGAAGGCGGATCTCTGGGC3593440.17118135514234661No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACT3507830.1671031359947509No Hit
GGGGTGATGGGGACTCACAGGAGACCGCCGG3447160.16421298816523766No Hit
GACGGACGAACCTCTGGTGTGCCAGT3407190.1623089300022964No Hit
CGGGACGGACGAACCTCTGGTGTGC3403110.1621145702998996No Hit
ACAAGCAGCGGAGCATGTGGCTTAATT3389450.16146384639432598No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGAT3254960.15505712179252543No Hit
ACACCGGTGGCGAAGGCGGATCTCTGGGC3125680.14889858690873647No Hit
ATTCGATACGGGCTAGCTAGAGTG3122490.14874662429828406No Hit
CTTGGGCTGCACACGTGCTACAATGGCCGATACAAT3109980.14815068315196445No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTAC3109050.14810638057274167No Hit
ACGTCGTCGGTGCCGCAGCTAACGCAT3044160.14501520383535718No Hit
CTTGGGCTGCACACGTGCTACAATGGCCGATAC2987580.1423198920800603No Hit
AGGACGAACGCTGGCGGCGTGCTTAACACATG2850940.13581074753704575No Hit
AGCGTCGTGTGTTTCTTGCCGGAGGTAGAG2849560.13574500822594093No Hit
TAATACCGGATAATACTCTCGCAGG2784990.13266907538678366No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACTC2733280.13020575670763201No Hit
AAGTGAGAGCGCGGCAGTGAGACTGTGGGG2717440.12945118374538558No Hit
GAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGT2717330.12944594365537No Hit
AACACGTGGGCAATCTGCCCTGCACTCTG2651790.1263238027497115No Hit
AAAAAGTCGGTCTCAGTTCGGATTGGGGT2625800.12508571239057106No Hit
TGCAGAAGAAGCGCCGGCTAACTACG2509850.11956218114230896No Hit
AACGGTGGGAACTAGGTGTTGGCG2496370.11892003192948813No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTC2491950.11870947558522492No Hit
CGTCGTCGGTGCCGCAGCTAACGCAT2466420.1174932983297861No Hit
TTAGTGGCGAACGGGTGAGTA2463920.1173742053748861No Hit
AGGGACAGTGTCTGATGGGTAGTTTA2418870.11522815032758804No Hit
ATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGT2331700.11107561717613473No Hit
ACCGCTGGAGGTGACTGGTACTAATAGG2286840.10893861319340907No Hit
AGGGACAGTGTCTGATGGGTAGTTTAAC2284480.10882618944398348No Hit
AAACGGTGGGAACTAGGTGTTGGC2277990.10851702413306308No Hit
AAGTGAGAGCGCGGCAGTGAGACTGTGGGGG2251720.10726559536297384No Hit
GTCCGGAATTATTGGGCGTAAAGAGCTCG2170010.10337316122502214No Hit
CTGCAGAAGAAGCGCCGGCTAACTACG2157090.10275768883409894No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAAC2156350.10272243731944852No Hit
TGGATGATCCTATGCTGTCGAGAAAGGCC2147290.10229084445089091No Hit
TCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAAC2112550.10063592874960048No Hit
CTTGGGCTGCACACGTGCTACAATGGCCG2102080.10013716745447927No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)