FastQCFastQC Report
Sat 24 Jun 2023
ERR3949730_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949730_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150989307
Total Bases4.8 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCC3958813526.219164645877868No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGA58389633.8671367635325327No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCC43091072.8539153438196787No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAAC39930202.6445713801441584No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGA20170911.3359164566534503No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAAC19768301.3092516544896786No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTC18739641.241123651226507No Hit
GTGGATGATCCTATGCTGTCGAGAAAAGCC15578611.0317690907740904No Hit
GCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGT9454460.6261675205913754No Hit
TGAATGATCCTATGCTGTCGAGAAAAGCC8752720.5796913817214884No Hit
TGCATGATCCTATGCTGTCGAGAAAAGCC8436250.558731619319241No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTC8020210.5311773501947393No Hit
GGATGATCCTATGCTGTCGAGAAAAGCC7601960.503476713089358No Hit
TGTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTC6200490.41065755735934334No Hit
GAATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAAC5898520.3906581278633195No Hit
GAATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA5845840.3871691390702257No Hit
TGGATGATCCTATGCTGTCGAGAAAAGC4741230.3140109782741105No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACT4690210.3106319310413154No Hit
TAGATGATCCTATGCTGTCGAGAAAAGCC4516510.2991278051233125No Hit
GACGTGGATGATCCTATGCTGTCGAGAAAAGCC4324710.28642491881892007No Hit
TCGATGATCCTATGCTGTCGAGAAAAGCC4119680.27284581152491816No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCC3751640.24847057546929463No Hit
GTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTC3671950.24319271827640088No Hit
TGGATGATCCTATGCTGTCTAGAAAAGCC3497550.2316422314594768No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA3434830.22748829491614264No Hit
TGGATGCTCCTATGCTGTCGAGAAAAGCC3248450.21514437442911108No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAAC3218170.21313893440149373No Hit
CTTGGGCTGCACACGTGCTACAATGGCCGATACAAT3134610.20760476766742164No Hit
TGGACGATCCTATGCTGTCGAGAAAAGCC2890060.1914082564800433No Hit
TGGAAGATCCTATGCTGTCGAGAAAAGCC2813370.1863290888539544No Hit
CCGCTGGAGGTGACTGGTACTAATAGGCCG2760070.1827990375503876No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACT2735950.1812015734332763No Hit
TGGATGCTCCTCTGCTGTCGAGAAAAGCC2548850.16880996745021157No Hit
TGGATGATCCTATGCTGTCGAGAAAGGCC2491040.1649812194978814No Hit
AGGATGATCCTATGCTGTCGAGAAAAGCC2434170.16121472760981678No Hit
TGGGTGATCCTATGCTGTCGAGAAAAGCC2415020.1599464258750456No Hit
TCGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTC2373490.1571958999719099No Hit
TAAGTGGATGATCCTATGCTGTCGAGAAAAGCC2357490.15613622228228388No Hit
GGGATGATCCTATGCTGTCGAGAAAAGCC2270680.15038680851750647No Hit
CGGATGATCCTATGCTGTCGAGAAAAGCC2228640.147602505388014No Hit
TGGATGATCCTCTGCTGTCGAGAAAAGCC2144500.14202992533769296No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACTCG2115520.14011058412235775No Hit
TGGTTGATCCTATGCTGTCGAGAAAAGCC2034130.1347201361749412No Hit
TGGATGATCCTATGCTGTCGAGAAGAGCC1981800.13125432783130794No Hit
TTGGATGATCCTATGCTGTCGAGAAAAGCC1925840.1275481051118408No Hit
TGGATGATCCTATGCTGTCGAGACAAGCC1914100.12677056660707767No Hit
AGGCGTTCGGGTGAACTATGGTTAAGG1823400.12076351870400995No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAAC1710050.11325636457156533No Hit
TGGATGATCCTATGCTGCCGAGAAAAGCC1565950.10371264237937061No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACTC1553880.10291324802225894No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)