FastQCFastQC Report
Sat 24 Jun 2023
ERR3949731_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949731_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144318010
Total Bases4.7 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCC2739579018.982932206451572No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCC132623049.189638909239395No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGA49341893.4189696767575994No Hit
GTGGATGATCCTATGCTGTCGAGAAAAGCC43906323.0423313070905014No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGA28693661.9882244773192201No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTC18233501.2634251262195204No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAAC18096251.2539148786766114No Hit
CTTGGGCTGCACACGTGCTACAATGGCCGATACAAT17908351.2408950206561191No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA15658841.0850232760277114No Hit
GAATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA15611781.0817624217517967No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTC14824141.0271857268541882No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAAC14057480.9740627659707891No Hit
GCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGT13680640.9479509868518835No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCC11442190.7928456053405947No Hit
TAATACCGGATAATACTCTCGCAGGCATCTGTG10678150.7399041879804191No Hit
GAATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAAC8154880.5650632239177911No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAAC5621870.3895473614138665No Hit
CTTGGGCTGCACACGTGCTACAATGGCCGATACA5264390.3647770642070245No Hit
CTAATACCGGATAATACTCTCGCAGGCATCTGTG5096010.35310977472596805No Hit
TGTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTC4986040.34548979715005773No Hit
TGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGG4805360.33297022318974606No Hit
GTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTC4710820.326419412241064No Hit
GACGTGGATGATCCTATGCTGTCGAGAAAAGCC4533540.31413542911241643No Hit
GGATGATCCTATGCTGTCGAGAAAAGCC4471970.30986915631666484No Hit
CCGCTGGAGGTGACTGGTACTAATAGGCCG4468230.3096100064018344No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA4444370.3079567130949214No Hit
CGGGACGGACGAACCTCTGGTGTGCCAGT3958490.27428939742170777No Hit
ACCGCTGGAGGTGACTGGTACTAATAGGCCG3920520.271658402163389No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGG3565460.24705579019555493No Hit
TGGATGATCCTATGCTGTCGAGAAAAGC3467380.24025968761625804No Hit
TGGCGAACGGGTGAGTAACACGTGGGCAATCTGCCCT3193140.2212572082999204No Hit
ACCGCTGGAGGTGACTGGTACTAATAGGCCGAGGGCT2655430.18399851827225167No Hit
TGAATGATCCTATGCTGTCGAGAAAAGCC2543050.1762115483715442No Hit
ACCCCGGGACCTTTACTACAGTTTGATATTG2429260.16832687756711723No Hit
TAAGTGGATGATCCTATGCTGTCGAGAAAAGCC2413840.1672584038540997No Hit
TACCGAGGCGTTCGGGTGAACTATGGTTAAGGA2336320.16188693289215947No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGC2253250.15613089454323822No Hit
GGCGAACGGGTGAGTAACACGTGGGCAATCTGCCCT2135180.14794965645659885No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAAC2024110.14025345831750313No Hit
TGCATGATCCTATGCTGTCGAGAAAAGCC2001670.13869855882852042No Hit
GGGGTGATGGGGACTCACAGGAGACCGCCGGGG1910890.13240828362309043No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGATACAAT1874220.1298673672121726No Hit
TGGATGCTCCTATGCTGTCGAGAAAAGCC1794060.12431296689858735No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACT1699890.11778779377570407No Hit
TTGGATGATCCTATGCTGTCGAGAAAAGCC1679480.11637355587150904No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACT1666760.11549216899540121No Hit
TGGATGATCCTATGCTGTCGAGAAAGGCC1659830.115011979447333No Hit
TTTCGGTGGTCATAGCGTGAGGGAAACGCCCGGT1648200.11420612022019982No Hit
TAGATGATCCTATGCTGTCGAGAAAAGCC1638410.11352775720784954No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTAC1604780.11119748671700781No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTACT1598140.11073739168105214No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACTCG1578510.10937720108529768No Hit
GGAGGTGACTGGTACTAATAGGCCGAGGGCT1565840.10849927878024371No Hit
TGGGTGATCCTATGCTGTCGAGAAAAGCC1554190.10769203372468898No Hit
AGGCGTTCGGGTGAACTATGGTTAAGG1539420.10666859943537194No Hit
GTAGGCGAAGGACATGCGAAAGGTCCGGCGTAGAGGG1476810.10233026356169961No Hit
GGGACGGACGAACCTCTGGTGTGCCAGT1470720.10190827880733667No Hit
AGACCCCGGGACCTTTACTACAGTTTGATATTG1460500.10120012048392298No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)