FastQCFastQC Report
Mon 26 Jun 2023
ERR3949732_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949732_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170463269
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCC4139901224.28617745210553No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGA118356586.943230685080902No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAAC66723133.9142233040245165No Hit
GCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGT40677172.3862718483945065No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGA39920452.341879880292569No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCC39361402.3090839587266156No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAAC33231831.9495009215152386No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTC19331701.1340683604982371No Hit
GAATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA15171920.890040422725907No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTC14001860.821400415593344No Hit
GAATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAAC12196700.7155031152195023No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA9913160.5815422910844212No Hit
GGATGATCCTATGCTGTCGAGAAAAGCC9780240.5737447168163835No Hit
TAATACCGGATAATACTCTCGCAGGCATCTGTG9134310.5358520960899793No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACT8780860.515117423918463No Hit
GTGGATGATCCTATGCTGTCGAGAAAAGCC7083380.4155370269239645No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAAC6346020.3722807873642268No Hit
TGAATGATCCTATGCTGTCGAGAAAAGCC6183970.36277434055309593No Hit
TGCATGATCCTATGCTGTCGAGAAAAGCC5958400.34954157778119344No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCC5368050.31490948352046444No Hit
TGTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTC5129650.30092406593469706No Hit
TGGATGCTCCTATGCTGTCGAGAAAAGCC5095610.29892715479954807No Hit
TGGATGATCCTATGCTGTCGAGAAAAGC5011220.2939765281633781No Hit
AGGCGTTCGGGTGAACTATGGTTAAGG4815970.2825224476951689No Hit
GTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTC4578000.2685622554850805No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACT4259570.2498819848397956No Hit
CCGCTGGAGGTGACTGGTACTAATAGGCCG4045630.23733148048451425No Hit
TAGATGATCCTATGCTGTCGAGAAAAGCC3807450.2233589689049082No Hit
GGGACGGACGAACCTCTGGTGTGCCAGT3348030.19640770822012102No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA3135790.18395693209426836No Hit
TGGATGCTCCTATGCTGTCGCGAAAAGCC3070620.18013382108728657No Hit
AGGCGTTCGGGTGAACTATGGTTAAG3069590.18007339751298562No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAAC2999980.17598981983620177No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACTCG2809000.1647862332148517No Hit
TGGAGGGAACGCGGGGAAGTGAAACATCTCAGTACCCGC2731940.16026561123851263No Hit
TGGATGATCCTATGCTGTCGAGAAAGGCC2671450.15671704618078164No Hit
TAATACCGGATAATACTCTCGCAGGCATCTGTGAGGGTTAAAAGCTCAGA2619830.153688827826011No Hit
TACCGAGGCGTTCGGGTGAACTATGGTTAAGGA2558180.150072212917611No Hit
TCGATGATCCTATGCTGTCGAGAAAAGCC2496280.14644093209311856No Hit
CGGGACGGACGAACCTCTGGTGTGCCAGT2467040.14472560654694472No Hit
TGGGTGATCCTATGCTGTCGAGAAAAGCC2463220.14450151134905198No Hit
TGGACGATCCTATGCTGTCGAGAAAAGCC2463070.1444927117993965No Hit
AGGCGTTCGGGTGAAC2421310.1420429171753124No Hit
ACCCCGGGACCTTTACTACAGTTTGATATTG2410670.14141873578641742No Hit
ACCGCTGGAGGTGACTGGTAC2249590.13196919272972524No Hit
CTTGGGCTGCACACGTGCTACAATGGCCGATACAAT2248910.13192930143795378No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTACT2195940.1288218871362839No Hit
TGGATGATCCTACGCTGTCGAGAAAAGCC2032310.11922275173544865No Hit
TGGATGATACTATGCTGTCGAGAAAAGCC2019510.11847185683151484No Hit
AGACCCCGGGACCTTTACTACAGTTTGATATTG1950670.11443345017629575No Hit
GGGATGATCCTATGCTGTCGAGAAAAGCC1914790.11232859789870625No Hit
TGGATGATCCTATGCTGTCGAGAAGAGCC1894010.11110956695310119No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTCG1873360.10989816228386422No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAACTC1828670.10727648312317652No Hit
GCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCG1799760.10558051658624475No Hit
AGAATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA1769910.10382940620480532No Hit
GACGTGGATGATCCTATGCTGTCGAGAAAAGCC1759150.10319818517618595No Hit
TACCGAGGCGTTCGGGTGAACTATGGTTAAGGAAC1737110.10190523801347491No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)