FastQCFastQC Report
Sat 24 Jun 2023
ERR3949734_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949734_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221698272
Total Bases6.9 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCC7170215532.34222547300684No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGA70886333.197423658764467No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCC65185542.940281825922396No Hit
GGATGATCCTATGCTGTCGAGAAAAGCC46964322.1183890869478676No Hit
GTGGATGATCCTATGCTGTCGAGAAAAGCC17872240.8061515247173419No Hit
TGGATGATCCTATGCTGTCGAGAAAAGC17038060.7685247091145573No Hit
TGCATGATCCTATGCTGTCGAGAAAAGCC16408150.74011176776335No Hit
TCGATGATCCTATGCTGTCGAGAAAAGCC15778700.7117195753334514No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAAC15612940.7042427466462166No Hit
TAGATGATCCTATGCTGTCGAGAAAAGCC13338640.6016573733150252No Hit
TGAATGATCCTATGCTGTCGAGAAAAGCC11120810.5016191556062286No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGA10817800.48795148028939084No Hit
TAATACCGGATAATACTCTCGCAGGCATCTGTG10245040.4621163668790346No Hit
TGGACGATCCTATGCTGTCGAGAAAAGCC10105480.4558213245793815No Hit
TGGAAGATCCTATGCTGTCGAGAAAAGCC8238740.37161949552768725No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTC7801180.35188276072805835No Hit
CTAATACCGGATAATACTCTCGCAGGCATCTGTG7739970.3491218010034828No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA7544360.34029854774871676No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCC6110470.27562100258499084No Hit
GGGATGATCCTATGCTGTCGAGAAAAGCC5597560.25248550426229754No Hit
TGATGATCCTATGCTGTCGAGAAAAGCC5555440.2505856247720325No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTC5328830.24036407464646364No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAAC5304090.2392481435308616No Hit
TAAGTGGATGATCCTATGCTGTCGAGAAAAGCC5264770.23747456182247553No Hit
GGGACGGACGAACCTCTGGTGTGCCAGT5224980.23567978012927407No Hit
TGGGTGATCCTATGCTGTCGAGAAAAGCC5139680.23183220841703267No Hit
GCAGTGGATGATCCTATGCTGTCGAGAAAAGCC4885410.2203630166319023No Hit
AGGATGATCCTATGCTGTCGAGAAAAGCC4566650.20598491629199525No Hit
TGGATGATCCTATGCTGTCGAGAAAGGCC4420130.19937593379167157No Hit
TGGTTGATCCTATGCTGTCGAGAAAAGCC4313820.19458067765183124No Hit
TACGTGGATGATCCTATGCTGTCGAGAAAAGCC4136750.18659369613850668No Hit
TGCTGATCCTATGCTGTCGAGAAAAGCC4083970.18421298295008812No Hit
CGGATGATCCTATGCTGTCGAGAAAAGCC4082860.18416291490084324No Hit
GGATTGATCCTATGCTGTCGAGAAAAGCC3681170.16604414489978522No Hit
GGAGGATCCTATGCTGTCGAGAAAAGCC3593490.1620892200729467No Hit
GACGTGGATGATCCTATGCTGTCGAGAAAAGCC3570930.1610716208018076No Hit
TGGATGATCCTATGCTGTCGAGAGAAGCC3358750.15150095531642213No Hit
TTGGATGATCCTATGCTGTCGAGAAAAGCC3338440.15058484533429292No Hit
GAATTGGATGATCCTATGCTGTCGAGAAAAGCC3063910.13820179888456688No Hit
TGTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTC3058190.1379437905587284No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAAC2923620.13187382894892388No Hit
GGGCGTTCGGGTGAACTATGGTTAAGGA2868830.12940245199565653No Hit
CTTGGGCTGCACACGTGCTACAATGGCCGATACAAT2838500.12803437637980325No Hit
GCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGT2814100.1269337814234294No Hit
GGCTGATCCTATGCTGTCGAGAAAAGCC2778950.12534829319734167No Hit
TGGATGATCCTATGCTGTCGAGAAGAGCC2722080.12278309503467849No Hit
GAATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA2701360.12184849144877413No Hit
CGGCGTTCGGGTGAACTATGGTTAAGGA2647420.11941545489357716No Hit
ACAAGCAGCGGAGCATGTGGCTTAATTC2574280.11611637640549584No Hit
TAATTGGATGATCCTATGCTGTCGAGAAAAGCC2502740.11288946807848824No Hit
TGGATGATCCTATGCTGCCGAGAAAAGCC2421510.10922547921347805No Hit
TGCGGATCCTATGCTGTCGAGAAAAGCC2420320.10917180265617948No Hit
TGGATGATCCTATGCTGTCTAGAAAAGCC2398810.10820156505324498No Hit
AGGCGTTCGGGTGAACTATGGTTAAGG2381400.10741626348806184No Hit
TCCGTGGATGATCCTATGCTGTCGAGAAAAGCC2368470.10683303837388503No Hit
TGGACGGACGAACCTCTGGTGTGCCAGT2363070.10658946408026129No Hit
TGGATGATCCTATGCCGTCGAGAAAAGCC2346530.10584340504016197No Hit
TGGATGGTCCTATGCTGTCGAGAAAAGCC2320000.10464673355685876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)