FastQCFastQC Report
Thu 29 Jun 2023
ERR3949735_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949735_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245481924
Total Bases8 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCC3715483115.135465126955744No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCC2618548010.66696870112522No Hit
GTGGATGATCCTATGCTGTCGAGAAAAGCC161088486.562132045209161No Hit
GGATGATCCTATGCTGTCGAGAAAAGCC88865343.620035990918826No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGA23614160.9619510722101069No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAG21026830.8565530877947657No Hit
CTAATACCGGATAATACTCTCGCAGGCATCTGTG20821630.8481940201837427No Hit
CTGGAAACGGGGTCTAATACCGGATAATACTC17829710.7263145778505466No Hit
TAATACCGGATAATACTCTCGCAGGCATCTGTG13289930.5413812057298361No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCC12145140.49474681484083527No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAAC10699120.4358414593491617No Hit
CGGGTGTAGTTTAATGGTAGAACATGAG10234280.41690564556598475No Hit
GCCCTCATCGTCTAGCGGCCTAGGACGCCG8641740.3520316224994228No Hit
GGGACGGACGAACCTCTGGTGTGCCAGT7934180.3232083189962288No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGG7603590.3097413396515501No Hit
TGGATGATCCTATGCTGTCGAGAAAAGC6781110.27623663239660773No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTC6749370.2749436655058969No Hit
ACCGCTGGAGGTGACTGGTACTAATAGGCCGAGGGCT6637900.27040280163357366No Hit
TGGATGATCCTATGCTGTCGAGACAAGCC6431620.2619997389298611No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTC6222820.25349402100987284No Hit
TGGATGATCCTATGCTGTCGAGAAACGCC6089080.24804596203181137No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGA6012260.24491660738327928No Hit
TTGGATGATCCTATGCTGTCGAGAAAAGCC5609670.22851662185929422No Hit
TGGCTGATCCTATGCTGTCGAGAAAAGCC5599540.2281039641843446No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA5224510.21282666824788288No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA4474270.18226474385951122No Hit
GAAGTTGATGATCCTATGCTGTCGAGAAAAGCC4359330.17758252538382419No Hit
TGGATGATCCTATGCTGTCGAGAACAGCC4183960.1704386185273666No Hit
TAAGTGGATGATCCTATGCTGTCGAGAAAAGCC4047980.16489931046817116No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGAAC3814490.15538781584586242No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGC3766320.15342555323951268No Hit
CGGGACGGACGAACCTCTGGTGTGCCAGT3759640.15315343544398813No Hit
ACAAGCAGCGGAGCATGTGGCTTAATTC3745610.15258190660099274No Hit
TGGATGATCCTATGCTGTCGAGACACGCC3428740.13967382787825958No Hit
TGGATTATCCTATGCTGTCGAGAAAAGCC3326550.1355109959134914No Hit
GAAGTGGATGATCCTATGCTGTCTAGAAAAGCC3258730.13274826703737258No Hit
GGGGTGATGGGGACTCACAGGAGACCGCCGGGG3081780.12553999698975798No Hit
GCAGTGGATGATCCTATGCTGTCGAGAAAAGCC2958580.12052129752738944No Hit
TGGATGATCATATGCTGTCGAGAAAAGCC2817940.11479215879047779No Hit
GTGGATGATCCTATGCTGTCGAGAACAGCC2795080.11386092932854804No Hit
GAAGTGGATGATCCTATGCTGTCGCGAAAAGCC2788960.113611623803307No Hit
GATGATCCTATGCTGTCGAGAAAAGCC2695210.10979260534066859No Hit
GTGGATGATCCTATGCTGTCGAGAAAAGC2629790.10712764333719332No Hit
TGGGTGATCCTATGCTGTCGAGAAAAGCC2588760.10545623717695808No Hit
GAAGTGGATTATCCTATGCTGTCGAGAAAAGCC2489530.1014139843551169No Hit
TGTATGATCCTATGCTGTCGAGAAAAGCC2488060.10135410214562274No Hit
TTGAATGATCCTATGCTGTCGAGAAAAGCC2486750.10130073772763813No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)