FastQCFastQC Report
Mon 26 Jun 2023
ERR3949745_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949745_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196272522
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGTAGTTTAATGGTAGAACATGAG2621423713.356040230634017No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCAAC84300454.295071421153899No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCC59038393.007980403899839No Hit
TGGCCTATGGTGTAATTGGCAGCACGACT54676952.785766924623305No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGC44772772.2811532426327106No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCC41048212.091388523555019No Hit
GGTCCTGTGGAGCAGTTTGGAGTGCTCGCC39585462.0168620444995353No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGGG31549201.607418077605382No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCG24844941.265838933888056No Hit
ACCGTTGGACGCGGGTTCGATTCCCGCCATCTC22571431.1500045839325383No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTG22273371.1348185560075494No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGG22013111.1215584217132544No Hit
GCACTCGTAGCTTAACGGATAGAGCATCT21543171.0976151822209734No Hit
GGGGCGGTAGCTCAGCCGGTTAGAGCAGCG20572211.0481451906956187No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCA20513671.0451626030463907No Hit
GGGGCTATAGCTCAGTTGGTAGAGCGCTTG18077810.9210565908966106No Hit
CGGATCTCTGGGCCATTACTGACGCTGAGGAGC17298850.8813689162255732No Hit
GCCCCAATAGCTCAGTCGGTAGAGCGTCTC16112000.8208994226914759No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGGT15021040.7653154831321727No Hit
ACCGGGACGGACGAACCTCTGGTGTGCCAG11976540.6101995265542062No Hit
ACCGTTGGACGCGGGTTCGTTTCCCGCCATCTC11370770.5793358073831649No Hit
GTCCGGGTGGCGGAATGGCAGACGCGCTAG10498710.5349047280291227No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGA10129880.5161129992511126No Hit
AGGGTCGTAGCTCAATTGGTAGAGCAC9797350.4991707397533721No Hit
TCGGGTGTAGTTTAATGGTAGAACATGAG9782750.49842687607590835No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCAATC9622110.4902423376411294No Hit
GGTGGGTGTAGCTCAGCTGGTAGAG9485430.4832785508304622No Hit
GGAGAAGGTCTACGGTTCGTTTCCGTATTGGGG9425290.480214443874115No Hit
AGACCCCGGGACCTTTACTACAGTTTGATATTGG8749210.4457684606508495No Hit
CACCGTTGGACGCGGGTTCGATTCCCGCCATCTC8339110.4248740432448308No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGG8328070.4243115600257075No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCG7373290.3756659324935968No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTCG7200580.36686643278573655No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCC6873440.35019879145385413No Hit
TCCAGGATGCCGCGGGTTCGAGTCCCGTCACTCAC6605460.3365453265027083No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCC6455430.3289013629732643No Hit
GCGGGTGTAGTTTAATGGTAGAACAT6172520.3144872209875614No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACT5957000.30350657031858996No Hit
GGGCCTGTGGCGCAGTCCGGTAGCGCACCTC5767740.2938638552777143No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCT5659490.2883485646554233No Hit
CGCGGGGTGGAGCAGCTCGG5353340.27275035473381243No Hit
ACCCTGTGGGAGAGTAGGACGCCGCCGA5232790.26660838443804175No Hit
AACGTTGGGAACTAGGTGTTGGCG5179720.26390449092002805No Hit
ACCGAAGAGGTCACTGGTTCGATCCCAGTATCGCC4776260.2433483786385544No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGG4752520.24213883591917162No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTC4746940.241854537335593No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGG4639350.23637287342748872No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGG4586860.23369853065830581No Hit
AGCGGGTGTCGCAGGTTCGAATCCTGCCGGGGG4575890.233139613908869No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTACT4271940.21765349303454712No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCT4266770.21739008377341787No Hit
TGCAAGAGGTCAGGAGTTCATTTCTCCTTAGCTC4187580.2133553875666814No Hit
TCCCCTGTAGCTCAATTGGCAGAGCATTCG4172060.21256465028762409No Hit
AGGAGAAGGTCTACGGTTCGATTCCGTATTGGGG4135200.21068664924986288No Hit
GCGGACGTAGCTCAGTTGGTAGAGCG4095090.20864306211951564No Hit
CGAAGAGGTCACTGGTTCGATCCCAGTATCGCC4079950.20787168567589914No Hit
GCGGACGTAGCTCAGTTGG3962890.20190752936878245No Hit
AGCGGAAGGTCGCCGGTTCGACCCCGGCCCCAGC3921700.19980891670612963No Hit
GGTCCTGTGGAGCAGTTTGGAGTGCTCGCCAC3872170.19728538465511744No Hit
CGGGACGGACGAACCTCTGGTGTGCCAG3794570.1933316982598308No Hit
CGGATCTCTGGGCCATTACTGACGCT3763560.19175175218872462No Hit
TCCCCTGTAGCTCAATTGGCAGAG3698510.18843748285865508No Hit
CACCGTTGGACGCGGGTTCGTTTCCCGCCATCTC3581270.18246415562948745No Hit
ACGTCGTCGGTGCCGCAGCTAACGCAT3514730.17907397144466305No Hit
TACCTGCAGAAGAAGCGCCGGCTAACTACGTG3502790.17846563361528517No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCT3469830.17678633588862736No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTAC3437540.17514117437182572No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGC3417680.17412931597220724No Hit
ATGCAAGAGGTCAGGAGTTCATTTCTCCTTAGCTC3353590.1708639582264093No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTG3261700.1661822025194133No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGT3243810.16527071476668548No Hit
GTCCGGGTGGCGGAATGGCAGACGCGC3164940.16125232242137288No Hit
GCGGGTGTAGTTTAATGGTAGAACATGGG3157080.16085185882514924No Hit
GGAGAAGGTCTACGGTTCGTTTCCGTATTGGG3127750.15935750802651835No Hit
TCGCAGGTCGTCGGTTCGTTTCCGACAGGCGG3110760.15849187488404515No Hit
TCCCCTGTAGCTCAATTGG3019180.1538259135428035No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGC2986050.15213795438976424No Hit
GCGGGTGTAGTTTAATGGTAGAACATG2915320.14853429151942113No Hit
TGGCCTATGGTGTAATTGGCAGCACGAC2898240.14766407291592248No Hit
GAGAAGGTCTACGGTTCGTTTCCGTATTGGGG2895180.1475081672410568No Hit
CGGGACGTGGCGCAGCTTGGTAGCGCACTTG2889430.14721520723109677No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAAC2828490.14411034062118996No Hit
TCGGACGTAGCTCAGTTGGTAGAGCGCAAC2789350.14211617457078377No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCATC2777970.14153636849889767No Hit
ACGTGGGCAATCTGCCCTGCACTCTGGGACAAGCC2677130.13639861416770302No Hit
AGCGGTGTAGCTCAGTCGGTAGAGCAAGCG2660100.1355309430425518No Hit
GGGGCTATAGCTCAGTTGGTTAGAGCG2640490.13453182203467076No Hit
CGGATCTCTGGGCCATTACTGACGCTG2475400.1261205580269662No Hit
ACCGGTGGAGTTGACTGGTACTAATAGGCCGAGGG2436580.12414269583798389No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACT2371210.12081212264649047No Hit
ACAAGCAGCGGAGCATGTGGCTTAATTC2361100.12029702252463031No Hit
GGGGCTATAGCTCAGTTGGTTAGAGCGC2350850.11977478946339723No Hit
CCGTTGGACGCGGGTTCGATTCCCGCCATCTC2315050.11795079496660262No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCG2282860.11631072840649595No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGG2258210.11505482158118904No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA2251910.11473383930940674No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAAC2232400.11373981325822062No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCTTG2191520.1116569949613222No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA2153940.10974231023535734No Hit
TAACACATGCAAGTCGAACGATGAACCC2083460.10615138475675165No Hit
GTTTCTTGCCGGAGGTAGAGCACTGGATAG2073100.10562354724315409No Hit
AGGGTCGTAGCTCAATTGGTAGAGC2052750.10458672355573033No Hit
CCGAAGAGGTCACTGGTTCGATCCCAGTATCGCC2046790.1042830641367109No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)