FastQCFastQC Report
Sat 24 Jun 2023
ERR3949746_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949746_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200168124
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGG85491704.270994716421482No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAG69671133.4806306122946924No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCAAC69046373.449418849526711No Hit
ACGAAGAGGTCGTGGGTT51804342.5880414406041994No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCC48705502.433229578551678No Hit
AGGATGAGGCCACAGGTT35371671.767098042043897No Hit
GGTCCTGTGGAGCAGTTTGGAGTGCTCGCC33162571.6567358147394136No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCC31551891.5762694563695865No Hit
GCGGGTGTAGTTCAG29106331.454094159367752No Hit
CGGAAGAGGTCACTGGTT26361441.3169649329380737No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCG24846201.2412665664988698No Hit
ATTCCGGTACCCGCTTTGAAACGC19823640.9903494924096905No Hit
ACCGTTGGACGCGGGTTCGATTCCCGCCATCTC19020590.9502307170546296No Hit
AAGGGCGTGGGGGTT18809510.9396855815064741No Hit
GAAGAGGTCGTGGGTT18105780.9045286351387297No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGC16057230.8021871654250005No Hit
GCGGACGTAGCTCAGTTGGTAGAGCG15729470.7858129299348381No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCA15390140.7688606803348969No Hit
GGTGGGTGTAGCTCAGCTGGTAGAG13962040.6975156543906061No Hit
GGGGCTATAGCTCAGTTGGTTAGAGCG13861480.6924918774779545No Hit
ACGGAAGAGGTCACTGGTT13413280.6701006999496084No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTG13250010.6619440565871517No Hit
TCCCCTGTAGCTCAATTGG12726830.6358070278962099No Hit
AGGGTCGTAGCTCAATTGGTAGAGCAC12190170.6089965653072714No Hit
TGGCCTATGGTGTAATTGGCAGCACGACT12137060.6063432956987697No Hit
CGCGGGGTGGAGCAGC11431560.5710979236634101No Hit
ACGTGGGCAATCTGCCCTGCACTCTGGG11363610.5677032772710604No Hit
CGCGGGGTGGAGCAGCT11314360.565242845559166No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCT9551190.47715839111326236No Hit
ACCCTGTGGGAGAGTAGGACGCCGCCGA9482470.47372527705759987No Hit
GAAGAGGTCGTGGGT9206290.4599278754293566No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGGG8975320.4483890751756259No Hit
GGGGCGGTAGCTCAGCCGGTTAGAGCAGCG8713230.43529558182800376No Hit
GCCCCAATAGCTCAGTCGGTAGAGCGTCTC8394060.41935048559479926No Hit
ACCGTTGGACGCGGGTTCGTTTCCCGCCATCTC8285440.41392404716747005No Hit
AGGCGTTCGGGTGAAC8037990.4015619390028354No Hit
CGGGACGGACGAACCTCTGGTGTGCCAG7943900.39686139037802043No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGC7928420.39608804047141893No Hit
CGGGGTGTGGCGCAGCT7575470.3784553628528786No Hit
CGGATCTCTGGGCCATTACTGACGCTGAGGAGC7568270.3780956652219012No Hit
AGGTGGAGGCCGCGGGTT7064160.35291133567300653No Hit
AGGCGTTCGGGTGAACTATGGT7025000.35095498022452365No Hit
ATCGAGGGGGCCAGGGGTT6968490.34813185340139374No Hit
ACCGGGACGGACGAACCTCTGGTGTGCCAG6967300.3480724033762738No Hit
CGCGGGGTGGAGCAGCTCGG6459450.32270123089128816No Hit
GTCCGGGTGGCGGAATGGCAGACGCGCTAG6431860.3213228895525843No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCC5978980.29869790856410283No Hit
GCGGACGTAGCTCAGTTGG5956480.2975738534672983No Hit
ACGAAGAGGTCGTGGGT5879110.29370860267441984No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCC5599770.2797533337525809No Hit
GCACTCGTAGCTTAACGGATAGAGCATCT5407070.2701264263235039No Hit
ACCAGCGGTTGGGGGTT5177840.25867455299725944No Hit
GCGGGTGTAGTTCAGTAG5064550.2530148106898379No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGA4983950.2489881955430626No Hit
TCGGGCGTGGGGGTT4930230.2463044515519364No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTAC4901320.24486016564755336No Hit
ATGCAAGAGGTCAGGAGTT4757250.23766271596770322No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTG4578590.2287372189190323No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGG4505380.22507979342405188No Hit
CGGATCTCTGGGCCAT4460890.22285716181263707No Hit
AACGTTGGGAACTAGGTGTTGGCG4372380.21843537885182956No Hit
ACCGTTGGACGCGGGTTCGATTCCCGC4368320.2182325493543617No Hit
AGGGGGCCAGGGGTT4186690.20915867703291258No Hit
TCGAGGGGGCCAGGGGTT4132200.20643646537847354No Hit
GTCCGGGTGGCGGAATGGCAGACGCGC4097740.204714912550212No Hit
ATGGTGTAATTGGCAGCACGACT4023010.20098155088869193No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTCG3998890.1997765638249175No Hit
CGGGGTGTGGCGCAG3626940.18119468412463116No Hit
ACGTCGTCGGTGCCGCAGCTAACGCAT3565830.17814175048171008No Hit
TGGAGGCCGCGGGTT3537580.1767304368601666No Hit
ACCCAGAGGTCGCAGGTT3450930.17240157578736162No Hit
GGGGCTATAGCTCAGTTGGTTAGAGCGC3441840.1719474575282526No Hit
AGCGGGGCTCAAGCG3440440.1718775163222292No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGG3373730.16854481785521455No Hit
TCCCCTGTAGCTCAATTGGCAGAG3370550.16838595140153284No Hit
ATTCCGGTACCCGCTTTGAAACG3298860.1648044620730921No Hit
CGGGGTGTGGCGCAGCTTGG3298170.16476999105012344No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGC3225330.16113105001673492No Hit
AGCCTGCTTCGAGATGAGGACTCC3202680.15999950121928502No Hit
TGCAAGAGGTCAGGAGTT3200590.15989508899029298No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACT3090720.15440620305758573No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGGT3068350.1532886425013405No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCG3027130.15122937356399463No Hit
GAGGGGGCCAGGGGTT2907640.14525989162989808No Hit
ACGTTGGGAACTAGGTGTTGGCG2877410.1437496611598358No Hit
AGCGGAAGGTCGCCGGTTCGACCCCGGCCCCAGC2870670.14341294421083747No Hit
AGCGGGTGTCGCAGGTTCGAATCCTGCCGGGGG2818880.140825619168015No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAG2781200.13894320156589968No Hit
TCTTGGGCTGCACACGTGC2776750.1387208884467539No Hit
GGATGATCCTATGCTGTCGAGAAAAGCC2772190.13849307994713483No Hit
AAGAGGTCAGGAGTT2733170.1365437186192543No Hit
TCCCCTGTAGCTCAATTGGCAGAGCATTCG2705350.13515388693956087No Hit
CGCGGGGTGGAGCAG2687070.13424065462091256No Hit
CGGGACGTGGCGCAGCTTGG2665190.13314757348677556No Hit
CGGGACGTGGCGCAGCT2605110.13014609658828596No Hit
ACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGA2586390.1292108827477446No Hit
ATTCCGGTACCCGCTTTGAAAC2582070.12899506416915812No Hit
GGGGCTATAGCTCAGTTGGTAGAGCGCTTG2566250.1282047285410938No Hit
AGCCTGCTTCGAGATGAGGACTCCCACC2563580.1280713406696063No Hit
GGTCCTGTGGAGCAGTTTGGAGTGCTCGCCAC2497150.12475263044379635No Hit
ACTGACGCTGAGGAG2492720.1245313164847366No Hit
GGGCCTGTGGCGCAGTCCGGTAGCGCACCTC2469430.12336779456453316No Hit
ATGGGCTGTCACCCGCTGCTGAACACATAGG2466660.12322941089261544No Hit
AATCTGCCCTGCACTCTGGG2383180.11905891669344916No Hit
AGGCGGCTTGTCACGTCGGGTGT2360350.11791837545522482No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGG2352460.11752420680127872No Hit
GGAAGGTCGCCGGTT2292150.11451123956179957No Hit
ATGCAAGTCGAACGATGAACCC2241000.1119558876417306No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCT2229880.11140035463388766No Hit
TCGCAGGTCGTCGGTTCGTTTCCGACAGGCGG2192600.10953792023349332No Hit
GATATTCCGGTACCCGCTTTGAAACGC2139100.10686516700331367No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCAATC2080060.10391564642929861No Hit
GGGTGCGTAGCTCAGGGGTAGAGCGCTG2054050.10261623873739256No Hit
AGGGTCGTAGCTCAATTGGTAGAG2048790.10235345963476182No Hit
ATTCGATACGGGCAGGC2048560.10234196929377226No Hit
GCGGACGTAGCTCAGTTGGTAGAGC2036620.10174547072240134No Hit
ATGCGTAGGTCTCGGGTT2030550.10144222563628563No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTC2022880.10105904774328604No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAG2008670.1003491445021486No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)