FastQCFastQC Report
Mon 26 Jun 2023
ERR3949747_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949747_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162668883
Total Bases4.8 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGTAGTTTAATGGTAGAACATGAG3458638821.26183407800249No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCAAC111403826.848502180961064No Hit
GCACTCGTAGCTTAACGGATAGAGCATCT56765393.4896280685716636No Hit
TGGCCTATGGTGTAATTGGCAGCACGACT34784732.1383763974084706No Hit
GGTCCTGTGGAGCAGTTTGGAGTGCTCGCC27515951.6915312561653233No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCA26107101.6049228050579285No Hit
GGGGCGGTAGCTCAGCCGGTTAGAGCAGCG24794251.5242159128860557No Hit
GCGGGTGTAGTTTAATTGTAGAACATGAG20485441.2593336612509967No Hit
GCCCCAATAGCTCAGTCGGTAGAGCGTCTC20030501.2313664193538476No Hit
GGTGGGTGTAGCTCAGCTGGTAGAG16338951.0044299621827488No Hit
GTCCGGGTGGCGGAATGGCAGACGCGCTAG14783400.90880319132701No Hit
GCGGACGTAGCTCAGTTGGTAGAGCG14571560.8957804179426252No Hit
GCGGGTGTAGTTTAATGGTAGAACATTAG13101030.8053802152191578No Hit
GCGGGTGTAGTTTAATGGTATAACATGAG12880380.7918158508532944No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAT12245090.7527616698517565No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCG11490690.7063852525501143No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGG11444410.7035402093466149No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGC10626050.6532318784041814No Hit
AGCGGTGTAGCTCAGTCGGTAGAGCAAGCG10343930.6358886720824166No Hit
GCGGGTGTAGTTTAATGGTAGAACAT8348900.5132450562164369No Hit
TTCTGGCGAAGCTGAGAACTTAAGCGC7869580.4837790642479545No Hit
GGGGCTATAGCTCAGTTGGTAGAGCGCTTG7415990.4558948130233365No Hit
ACCGTTGGACGCGGGTTCGATTCCCGCCATCTC6382530.3923633015909994No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCC6235280.38331117082792043No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAGC5857510.3600879216709197No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTG5405130.3322780546787181No Hit
ACCGTTGGACGCGGGTTCGTTTCCCGCCATCTC5252940.3229222395287487No Hit
GGTCCTGTGGAGCAGTTTGGAGTGCTCGCCAC4988310.3066542234755494No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGGG4964000.305159776624273No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCC4854120.2984049506259903No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGG4435910.27269566976740106No Hit
GCGGGTGTAGTTTAATGGTAGAACATTAT4349940.2674107007914968No Hit
GCGGGTGTAGTTTAATGGTAGAACATGGG3964240.24369995827659305No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTGGT3962250.24357762387782547No Hit
GGGCCTGTGGCGCAGTCCGGTAGCGCACCTC3880660.23856191352835443No Hit
GCGGACGTAGCTCAGTTGG3870120.23791397153689192No Hit
TCGCAGGTCGTCGGTTCGTTTCCGACAGGCGG3851590.2367748477131917No Hit
AGCTCAGAGCGCGAGTTCGATTCTCGTCACCCG3680970.22628605619674663No Hit
TGCAAGAGGTCAGGAGTTCATTTCTCCTTAGCTC3650840.2244338273350042No Hit
GCACTCGTAGCTTAACTGATAGAGCATCT3476380.2137089734611382No Hit
AGCGGTGTAGCTCAGTCGGTAGAGCAAGC3390870.20845228278846667No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCATC3231240.19863909682099434No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCC3175170.19519221755521615No Hit
AGCGGTGTAGCTCAGTCGGTAGAG3111960.1913064098436085No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGC3111610.19128489374332275No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGG3024470.18592799951789182No Hit
ACCGAAGAGGTCACTGGTTCGATCCCAGTATCGCC2916850.17931210605288292No Hit
ACTCGTAGCTTAACGGATAGAGCATCT2858380.17571768781371666No Hit
TCCAGGATGCCGCGGGTTCGAGTCCCGTCACTCAC2801220.17220380126419138No Hit
GTCCGGGTGGCGGAATGGCAGACGCGC2762430.1698192026068071No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACT2693600.16558790779918248No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCCAC2642950.16247422071497228No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGG2640570.16232791123302912No Hit
AGCTCAGAGCGCGAGTTCGTTTCTCGTCACCCG2638130.16217791327675127No Hit
CGGGACGTGGCGCAGCTTGGTAGCGCACTTG2622900.16124165554145964No Hit
GCGGGTGTAGTTTAATGGTAGAACATG2609470.16041605203620904No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCTTG2545050.15645586009218493No Hit
GCGGACGTAGCTCAGTTGGTAGAGC2499640.15366429976653861No Hit
GCGGGTGTAGTTTAATGGTATAACATGAT2463210.1514247810996526No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGG2400830.14758999728300834No Hit
AGGGTCGTAGCTCAATTGGTAGAGCAC2396480.1473225828937425No Hit
GCGGGTTTAGTTTAATGGTAGAACATGAG2365710.14543101030576328No Hit
TCCCCTGTAGCTCAATTGGCAGAGCATTCG2358300.14497548372542768No Hit
GCGGACGTAGCTCAGTGGGTAGAGCGCAAC2279060.1401042386207324No Hit
GGGTGCGTAGCTCAGGGGTAGAGCGCTG2194130.13488320319996297No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAC2011910.12368130664547564No Hit
GGTCCTGTGGAGCAGTTTGGAGTGCTCGCCACC2007130.12338745819014446No Hit
CGGATCTCTGGGCCATTACTGACGCTGAGGAGC1983330.121924363370713No Hit
CGAAGAGGTCACTGGTTCGATCCCAGTATCGCC1904850.11709983894092393No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTAC1885430.11590600274792567No Hit
AGGTTGAGGTCGCCAGTTCGAACCTGGTCGTCCG1875370.11528756855114079No Hit
GCGGGTGTAGTTTAATGGTAGAACATGA1860300.11436114674740837No Hit
TGGCGAAGCTGAGAACTTAAGCGCCAGTAAACGGCG1855960.11409434710386497No Hit
GCGGGTGTAGTTTAATGTTAGAACATGAG1847750.11358964086573337No Hit
GGAGAAGGTCTACGGTTCGTTTCCGTATTGGGG1844170.11336956189709621No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCAGC1843380.11332099698502264No Hit
TTCTGGCGAAGCTGAGAACTTAAGC1767300.1086440115286216No Hit
GCGGGTGTAGTTTAATTGTAGAACATTAG1722000.10585921340592226No Hit
ACGTCACGAAAGTCGGTAACACCCGAAGCC1711550.10521680412596182No Hit
AGCGGGTGTCGCAGGTTCGAATCCTGCCGGGGG1696210.10427378418772323No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)