FastQCFastQC Report
Mon 26 Jun 2023
ERR3949748_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949748_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186424116
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGTAGTTTAATGGTAGAACATGAG3231226517.332663656026135No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCAAC99143645.318176753483975No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCC47241292.534076117062022No Hit
TGGCCTATGGTGTAATTGGCAGCACGACT44903232.408659939682911No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCC39282252.107144228056846No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGC38504182.065407675045647No Hit
GGGGCGGTAGCTCAGCCGGTTAGAGCAGCG35445031.9013114161689253No Hit
GCACTCGTAGCTTAACGGATAGAGCATCT35144441.885187429291605No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGG29266981.5699138409753812No Hit
GGTGGGTGTAGCTCAGCTGGTAGAG25826251.3853492002075525No Hit
GCCCCAATAGCTCAGTCGGTAGAGCGTCTC25174311.350378402759866No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCA23191531.2440198455869305No Hit
GGTCCTGTGGAGCAGTTTGGAGTGCTCGCC21840121.1715286878442273No Hit
TCGGGTGTAGTTTAATGGTAGAACATGAG21818301.1703582384158926No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGG21718131.1649850065535512No Hit
GTCCGGGTGGCGGAATGGCAGACGCGCTAG14870890.7976913244421661No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCG14810870.794471783897315No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAGC10850770.5820475501141709No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAT10060520.5396576481553492No Hit
GCGGACGTAGCTCAGTTGGTAGAGCG9372550.5027541608404354No Hit
GCGGGTGTAGTTTAATGGTAGAACAT9156000.4911381744194512No Hit
CGGATCTCTGGGCCATTACTGACGCTGAGGAGC8919110.478431127440615No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTG8869170.47575228947310655No Hit
ACCGTTGGACGCGGGTTCGATTCCCGCCATCTC7707870.41345884670843763No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGG7461630.4002502551762133No Hit
ACCGGGACGGACGAACCTCTGGTGTGCCAG7250270.388912666213206No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGGT6729830.3609956771901764No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCC6678830.35825997962624107No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTGGT6135580.32911943645745917No Hit
GCGGACGTAGCTCAGTTGG6004660.3220967398874511No Hit
TCGGACGTAGCTCAGTTGGTAGAGCGCAAC5830780.31276962042829265No Hit
ACCGTTGGACGCGGGTTCGTTTCCCGCCATCTC5745610.30820100549652063No Hit
GTCCGGGTGGCGGAATGGCAGACGCGC5705400.3060440957113081No Hit
AGGGTCGTAGCTCAATTGGTAGAGCAC5571380.2988551116423156No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTAC5189060.2783470353159674No Hit
AGCGGTGTAGCTCAGTCGGTAGAGCAAGCG5052860.2710411135863989No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCC5048100.2707857818137649No Hit
GGGGCTATAGCTCAGTTGGTAGAGCGCTTG4725490.2534806172823692No Hit
GCGGGTGTAGTTTAATGGTATAACATGAG4398540.23594265025239547No Hit
CGCGGGGTGGAGCAGCTCGG4312180.23131020237746494No Hit
TTCTGGCGAAGCTGAGAACTTAAGCGC4147590.22248140900397242No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCT3974800.21321275837510206No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGG3954910.2121458363251673No Hit
GGGTGCGTAGCTCAGGGGTAGAGCGCTG3929630.21078978859151465No Hit
AGCGGTGTAGCTCAGTCGGTAGAG3832290.20556836112340746No Hit
TGGTGGAGCCGACGACCCGAGGTG3746400.2009611245789681No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGA3598670.1930367206354354No Hit
ACCCTGTGGGAGAGTAGGACGCCGCCGA3571610.19158519169268853No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCAATC3332270.17874672394852606No Hit
GCGGGTGTAGTTTAATGGTAGAACATG3259980.17486900675446948No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCG3256110.1746614155863826No Hit
TCCCCTGTAGCTCAATTGGCAGAGCATTCG3191240.17118171556731426No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACT3133940.16810807889253984No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTT3089600.16572963124577722No Hit
CGGCTTGTCACGTCGGGTGTGAAAGCCCGGGG3084650.1654641076586894No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCT2976650.15967086575859102No Hit
GGGCCTGTGGCGCAGTCCGGTAGCGCACCTC2944140.157926992664404No Hit
ACGTCACGAAAGTCGGTAACACCC2845780.1526508512450181No Hit
TCCCCTGTAGCTCAATTGGCAGAG2728970.1463850310010321No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTG2688810.14423080327225477No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGC2660190.14269559416872868No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGGG2510650.13467409978224062No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTACT2505790.1344134038967362No Hit
TGGCCTATGGTGTAATTGGCAGCACGAC2488640.13349345853945205No Hit
GCGGGTGTAGTTTAATGGTAGAACATGGG2416990.12965007166776643No Hit
GCGGGTGTAGTTGAATGGTAGAACATGAG2415340.1295615638054038No Hit
ACGTCACGAAAGTCGGTAACACCCGAAGCC2341720.12561250391017006No Hit
AACGTTGGGAACTAGGTGTTGGCG2341480.12559963003928096No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGC2292500.12297228755532895No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGG2206950.11838328899464916No Hit
TCGCAGGTCGTCGGTTCGTTTCCGACAGGCGG2200270.11802496625490234No Hit
TCCCTCATCGTCTAGTGGCCCAGGACGCCGC2153690.11552636247984138No Hit
GGTCCTGTGGAGCAGTTTGGAGTGCTCGCCACC2058590.11042509114003254No Hit
TCACTCGTAGCTTAACGGATAGAGCATCT2045640.1097304385233078No Hit
ACCGAAGAGGTCACTGGTTCGATCCCAGTATCGCC2033680.1090888906240006No Hit
GCGGACGTAGCTCAGTTGGTAGAGC2013860.10802572345307514No Hit
TGGGCGGTAGCTCAGCCGGTTAGAGCAGCG2001770.10737720220703635No Hit
TCCCCTGTAGCTCAATTGG1998250.1071883854339961No Hit
AGCGGTGTAGCTCAGTCGGTAGAGCAAGC1998010.107175511563107No Hit
CACCGTTGGACGCGGGTTCGATTCCCGCCATCTC1971670.10576260423302745No Hit
ACGGGTGTAGTTTAATGGTAGAACATGAG1950030.1046018102078596No Hit
CGGATCTCTGGGCCATTACTGACGCT1943960.10427620855662258No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCT1942790.10421344843603818No Hit
ACGTCACGAAAGTCGGTAACACCCGAAGC1915900.10277103848517109No Hit
GGGGCGGTAGCTCAGCCGGTTAGAGCAGC1891230.10144771184002827No Hit
GCGGGTGTAGTTTAATGGTAGAAAATGAG1881590.10093061135931576No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)