FastQCFastQC Report
Mon 26 Jun 2023
ERR3949749_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949749_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172769472
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGTAGTTTAATGGTAGAACATGAG3457706120.013408966139572No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCAAC137156487.938698799750919No Hit
GCACTCGTAGCTTAACGGATAGAGCATCT80173284.6404772250505No Hit
TGGCCTATGGTGTAATTGGCAGCACGACT65698123.8026463378900646No Hit
GGGGCGGTAGCTCAGCCGGTTAGAGCAGCG45788242.6502506183499825No Hit
GCCCCAATAGCTCAGTCGGTAGAGCGTCTC40601652.350047698241504No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGG34820282.0154185572784526No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCA32455311.8785326842927434No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGG31900481.846418793246066No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGC25566561.4798077289950855No Hit
GTCCGGGTGGCGGAATGGCAGACGCGCTAG19038581.1019643562955381No Hit
GGTCCTGTGGAGCAGTTTGGAGTGCTCGCC15230870.8815718323200062No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTG15123850.8753774509422589No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCG14378050.8322101024884766No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAGC13328010.7714331615252028No Hit
AGCGGTGTAGCTCAGTCGGTAGAGCAAGCG9925200.5744764908467163No Hit
GCGGGTGTAGTTTAATGGTAGAACAT9328920.5399634490982296No Hit
GCGGGTGTAGTTTAATGGTAGAACATG8504900.4922686804298389No Hit
GGTGGGTGTAGCTCAGCTGGTAGAG8438330.48841556915795864No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTGGT7533960.43607009460560253No Hit
GCGGACGTAGCTCAGTTGGTAGAGCG6863790.39728025562293784No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTGG6675470.3863801817950801No Hit
TTCTGGCGAAGCTGAGAACTTAAGCGC5720070.33108106043178737No Hit
GGGTGCGTAGCTCAGGGGTAGAGCGCTG5256430.3042453009290901No Hit
GTCCGGGTGGCGGAATGGCAGACGCGC4947500.2863642484246291No Hit
GCGGGTGTAGTTTAATGGTATAACATGAG4846000.2804893679364836No Hit
GCGGACGTAGCTCAGTTGG4593290.26586236253589984No Hit
TCGCAGGTCGTCGGTTCGTTTCCGACAGGCGG4113090.2380681003643977No Hit
CGGATCTCTGGGCCATTACTGACGCTGAGGAGC4094750.23700657023481556No Hit
AGGGTCGTAGCTCAATTGGTAGAGCAC4048560.2343330655082398No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCC3885090.2248713244895487No Hit
AGCGGTGTAGCTCAGTCGGTAGAGCAAGC3636610.210489154009801No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACT3589740.20777629047798443No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCT3571570.2067246000497125No Hit
GCCGGTGTAGTTTAATGGTAGAACATGAG3378180.1955310716004272No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCC3302700.19116224421870084No Hit
CGGGACGTGGCGCAGCTTGGTAGCGCACTTG3206510.1855947096950091No Hit
TCCCCTGTAGCTCAATTGGCAGAGCATTCG3172470.1836244542091325No Hit
GGGGCTATAGCTCAGTTGGTAGAGCGCTTG3147440.18217570289269622No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTG3070710.177734524766042No Hit
CGCGGGGTGGAGCAGCTCGG2994550.1733263385790749No Hit
ACTCGTAGCTTAACGGATAGAGCATCT2954970.17103542459167786No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGCC2899900.16784794017313429No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGGG2810600.1626792029554851No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGG2672190.15466794967110856No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGC2562090.1482952960578591No Hit
TGGCCTATGGTGTAATTGGCAGCACGAC2498000.1445857286639158No Hit
CGGCTTGTCACGTCGGGTGTGAAAGCCCGGGG2492650.14427606747562438No Hit
GGTCCTGTGGAGCAGTTTGGAGTGCTCGCCACC2487640.14398608568995339No Hit
GCCCCAATAGCTCAGTCGGTAGAGCGTCT2482970.14371578330690274No Hit
GGGGCGGTAGCTCAGCCGGTTAGAGCAGC2468710.14289040600876524No Hit
TTCTGGCGAAGCTGAGAACTTAAGC2454170.1420488221437639No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGC2436580.1410307024611385No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCC2384250.1380018108754769No Hit
GGGCCTGTGGCGCAGTCCGGTAGCGCACCTC2223530.1286992414956272No Hit
GCGGGTGTAGTTTAATGGTAGAACATGGG2213480.12811754150640686No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGA1982360.11474017817221782No Hit
AGCTCAGAGCGCGAGTTCGATTCTCGTCACCCG1969300.11398425758921114No Hit
ACCGTTGGACGCGGGTTCGATTCCCGCCATCTC1938780.11221774180105151No Hit
GCCGACGTAGCTCAGTTGGTAGAGCGCAAC1893750.10961137856576883No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTAC1876310.10860194097253477No Hit
TGGCGAAGCTGAGAACTTAAGCGCCAGTAAACGGCG1848920.10701659144967463No Hit
AGGTCGGCTCTGGCGGGTGCGTGGGGGGCG1839830.10649045683255895No Hit
GGTCCTGTGGAGCAGTTTGGAGTGCTCGCCAC1810780.10480902552043453No Hit
TGCCCTATGGTGTAATTGGCAGCACGACT1795230.10390898225353146No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGG1792120.10372897360015085No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCAACC1763910.10209616198861798No Hit
GGACGATTAGCTCAGCGGGAGAGCGCTTCC1755020.10158160349069076No Hit
ACCGTTGGACGCGGGTTCGTTTCCCGCCATCTC1743480.10091366141351639No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)