FastQCFastQC Report
Thu 29 Jun 2023
ERR3949750_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949750_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167661579
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGTAGTTTAATGGTAGAACATGAG3292325019.636729056452463No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCAAC119651257.136474003981556No Hit
TGGCCTATGGTGTAATTGGCAGCACGACT50720743.0251856330185225No Hit
GCACTCGTAGCTTAACGGATAGAGCATCT47338302.8234435272734726No Hit
GGGGCGGTAGCTCAGCCGGTTAGAGCAGCG35243462.102059410999583No Hit
GCCCCAATAGCTCAGTCGGTAGAGCGTCTC34041302.030357831712893No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGG28602331.7059561391820126No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCA27575071.6446862879658315No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGC25771501.5371142365300043No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTG17470271.041996031780185No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCC16682320.9949995759016441No Hit
GGTGGGTGTAGCTCAGCTGGTAGAG12086680.7208974215851802No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGG11953380.712946882123781No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCG11819970.7049897818271174No Hit
GTCCGGGTGGCGGAATGGCAGACGCGCTAG11061150.6597307544145221No Hit
GGTCCTGTGGAGCAGTTTGGAGTGCTCGCC10927970.6517873722279569No Hit
GCGGGTGTAGTTTAATGGTAGAACAT10216810.6093709758035859No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAGC9913060.591254123880105No Hit
GCGGACGTAGCTCAGTTGGTAGAGCG9163340.5465378564757523No Hit
GGTGTAGTTTAATGGTAGAACATGAG6676690.39822421092670257No Hit
CGGATCTCTGGGCCATTACTGACGCTGAGGAGC6315630.3766891638304325No Hit
GCGGGTGTAGTTTAATGGTAGAACATG5712240.340700596646534No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCC5603940.3342411561088781No Hit
AGGGTCGTAGCTCAATTGGTAGAGCAC5124500.30564545738889887No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACT4995160.2979311079970206No Hit
AGCGGTGTAGCTCAGTCGGTAGAGCAAGCG4811780.28699359917157885No Hit
GCCGGTGTAGTTTAATGGTAGAACATGAG4612290.2750952261996769No Hit
GCGGACGTAGCTCAGTTGG4464750.26629535679131355No Hit
ACCGGGACGGACGAACCTCTGGTGTGCCAG4150150.24753136793492803No Hit
GCGCGTGTAGTTTAATGGTAGAACATGAG4091510.24403384629939576No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTGGT3940670.23503715183309826No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCC3869900.2308161489997658No Hit
ACGTCGTCGGTGCCGCAGCTAACGCAT3507310.20918984664936263No Hit
CGCGGGGTGGAGCAGCTCGG3444920.20546866017526888No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCT3386020.20195563111092973No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTG3377120.2014247998940771No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTGG3208280.19135451420268446No Hit
GGGGCTATAGCTCAGTTGGTAGAGCGCTTG3207070.19128234501477528No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGGT3158200.1883675448386419No Hit
TTCTGGCGAAGCTGAGAACTTAAGCGC3151240.18795242289827177No Hit
GTCCGGGTGGCGGAATGGCAGACGCGC3085580.18403620068495238No Hit
CGGGACGTGGCGCAGCTTGGTAGCGCACTTG3000120.17893902812402834No Hit
TGGACTATGGTGTAATTGGCAGCACGACT2811750.16770389595340743No Hit
ACTCGTAGCTTAACGGATAGAGCATCT2785400.16613227768778202No Hit
GCAATCGTAGCTTAACGGATAGAGCATCT2737460.16327294639161188No Hit
ACCGTTGGACGCGGGTTCGATTCCCGCCATCTC2696000.160800107936476No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCT2623200.15645802787053556No Hit
GGGTGCGTAGCTCAGGGGTAGAGCGCTG2567060.15310961612737764No Hit
ACCGTTGGACGCGGGTTCGTTTCCCGCCATCTC2548550.1520056064842381No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGG2546470.15188154705378268No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGC2408820.14367155637965212No Hit
GCGGGTGTAGTTTAATTGTAGAACATGAG2336800.13937599860013247No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGC2292750.1367486822964968No Hit
GCCCCAATAGCTCAGTCGGTAGAGCGTCT2273190.13558204649856007No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGA2273030.1355725034654481No Hit
TCGCAGGTCGTCGGTTCGTTTCCGACAGGCGG2263160.13498381761035425No Hit
TTCACGGAGAGTTTGATCCTGGCTCAGG2258690.13471720912278895No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGG2255240.13451143747131236No Hit
AGCGGTGTAGCTCAGTCGGTAGAGCAAGC2177430.1298705411810538No Hit
GCCGACGTAGCTCAGTTGGTAGAGCGCAAC2150500.1282643294203975No Hit
GGGCCTGTGGCGCAGTCCGGTAGCGCACCTC2143730.1278605398318478No Hit
TCCCCTGTAGCTCAATTGGCAGAGCATTCG2134990.12733925164810717No Hit
GGGGCTATAGCTCAGTTGGTTAGAGCG2119270.1264016486448574No Hit
AACACATGCAAGTCGAACGATGAACCC2114070.12609150006871878No Hit
CGGCTTGTCACGTCGGGTGTGAAAGCCCGGGG2100810.12530062119956537No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAT2049630.12224804348287809No Hit
GCGGGTGTAGTTTAATGGTAGAACATGGG1968510.11740972569511587No Hit
GCGCACGTAGCTCAGTTGGTAGAGCGCAAC1934990.11541046025816087No Hit
CGAGGGGTGGAGCAGCTCGGTAGCTCGCTGG1894320.1129847405290153No Hit
AGGACGAACGCTGGCGGCGTGCTTAACACATG1833690.10936852741915308No Hit
TGGCCTATGGTGTAATTGGCAGCACGAC1788270.10665949889449627No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCC1787800.10663146623472991No Hit
GGGGCGGTAGCTCAGCCGGTTAGAGCAGC1785270.10648056702364708No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCATC1782830.10633503576868973No Hit
TCCCCTGTAGCTCAATTGGCAGAG1767640.10542904406262332No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGGG1764140.10522029021329926No Hit
ACGGGTGTAGTTTAATGGTAGAACATGAG1753870.10460774677542552No Hit
TGCCCTATGGTGTAATTGGCAGCACGACT1709720.10197446607609487No Hit
TTCTGGCGAAGCTGAGAACTTAAGC1709300.10194941561417598No Hit
GGGGGTGTAGTTTAATGGTAGAACATGAG1708280.10188857877808727No Hit
GCGGACGTAGCTCAGTTGGTAGAGC1689430.10076429018958481No Hit
GCACCAATAGCTCAGTCGGTAGAGCGTCTC1677900.10007659536595441No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)