FastQCFastQC Report
Sat 24 Jun 2023
ERR3949751_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949751_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168131662
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGTAGTTTAATGGTAGAACATGAG3615690921.505116032220034No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCAAC109641796.521186354536838No Hit
GCACTCGTAGCTTAACGGATAGAGCATCT57772253.4361315003238357No Hit
TGGCCTATGGTGTAATTGGCAGCACGACT41189742.4498502845942247No Hit
GGGGCGGTAGCTCAGCCGGTTAGAGCAGCG33440321.98893650382163No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGG31732821.8873791897685517No Hit
GCCCCAATAGCTCAGTCGGTAGAGCGTCTC28645431.7037498862052525No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCA27304071.6239695530994038No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGC21359901.2704269823966887No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGG18828831.1198860331256346No Hit
GGTGGGTGTAGCTCAGCTGGTAGAG13569350.8070669045072545No Hit
GCGGGTGTAGTTTAATGGTAGAACAT12711640.7560527177801882No Hit
GTCCGGGTGGCGGAATGGCAGACGCGCTAG10904060.648542925841059No Hit
GGTCCTGTGGAGCAGTTTGGAGTGCTCGCC10547350.6273268148625093No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTG10377830.617244240409638No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCC10296500.6124069599692651No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAGC10252020.6097614142421313No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCG8911730.5300447217371823No Hit
GCGGACGTAGCTCAGTTGGTAGAGCG8704960.5177466216922307No Hit
GCGGGTGTAGTTTAATGGTAGAACATG8565840.5094721540312853No Hit
GCGCGTGTAGTTTAATGGTAGAACATGAG7561390.4497302834013501No Hit
GCAATCGTAGCTTAACGGATAGAGCATCT6445440.38335670529444954No Hit
TTCTGGCGAAGCTGAGAACTTAAGCGC6295110.37441549825398146No Hit
CGGATCTCTGGGCCATTACTGACGCTGAGGAGC5435710.3233007950638114No Hit
AGCGGTGTAGCTCAGTCGGTAGAGCAAGCG5395450.3209062431084515No Hit
GCCGGTGTAGTTTAATGGTAGAACATGAG5376730.3197928299786866No Hit
GCGGACGTAGCTCAGTTGG5008970.29791949597215067No Hit
TGGACTATGGTGTAATTGGCAGCACGACT4689010.2788891719871299No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTGGT4240390.2522065118228594No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTGG3625300.21562268265688114No Hit
GTCCGGGTGGCGGAATGGCAGACGCGC3469920.20638111577104376No Hit
AGGGTCGTAGCTCAATTGGTAGAGCAC3404150.2024693005175908No Hit
GGGTGCGTAGCTCAGGGGTAGAGCGCTG3384100.2012767827156791No Hit
GCGCACGTAGCTCAGTTGGTAGAGCGCAAC3321530.19755529449295514No Hit
TGGTGGAGCCGACGACCCGAGGTG3315860.19721805878538212No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCT3203260.19052092639160373No Hit
CGCGGGGTGGAGCAGCTCGG3013590.17923988641711042No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCC2948240.17535305158644066No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTG2941740.17496644980527226No Hit
ACGTCACGAAAGTCGGTAACACCC2933780.17449301131633374No Hit
ACTCGTAGCTTAACGGATAGAGCATCT2916340.17345572899886044No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCC2835000.16861785378651642No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACT2820590.16776078737626468No Hit
CGAGGGGTGGAGCAGCTCGGTAGCTCGCTGG2760490.16418620783038473No Hit
GCCCTCGTAGCTTAACGGATAGAGCATCT2658900.15814391937670846No Hit
GCAGGTGTAGTTTAATGGTAGAACATGAG2412710.14350122822196335No Hit
TTCTGGCGAAGCTGAGAACTTAAGC2380660.14159498405481769No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGC2364130.14061182598670796No Hit
TCGCAGGTCGTCGGTTCGTTTCCGACAGGCGG2343800.1394026545696075No Hit
AGCGGTGTAGCTCAGTCGGTAGAG2327680.13844388215230988No Hit
ACCGGGACGGACGAACCTCTGGTGTGCCAG2323760.13821073153966681No Hit
CGGGACGTGGCGCAGCTTGGTAGCGCACTTG2288220.13609691195463233No Hit
AGCGGTGTAGCTCAGTCGGTAGAGCAAGC2246380.13360838602785No Hit
GCGGGTGTAGTTTAATGGTAGAACATGGG2189270.13021164330130752No Hit
GCCGACGTAGCTCAGTTGGTAGAGCGCAAC2173770.12928974674621369No Hit
GCGGACGTAGCTCAGTTGGTAGAGC2115680.12583471636651045No Hit
GCCCCAATAGCTCAGTCGGTAGAGCGTCT2092880.12447863627256595No Hit
TCCCCTGTAGCTCAATTGGCAGAGCATTCG2053950.12216318898935288No Hit
ACCGTTGGACGCGGGTTCGTTTCCCGCCATCTC2011730.1196520617276715No Hit
GCGGGTGTAGTTTAATGGTAGCACATGAG2005010.11925237496314049No Hit
CGGCTTGTCACGTCGGGTGTGAAAGCCCGGGG1974690.11744902634698276No Hit
ACCGTTGGACGCGGGTTCGATTCCCGCCATCTC1969560.11714390832584524No Hit
GGGGGTGTAGTTTAATGGTAGAACATGAG1965970.11693038518824611No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGC1950930.11603584814381958No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGG1908990.11354137449732697No Hit
GGGGCTATAGCTCAGTTGGTAGAGCGCTTG1903610.11322138717691377No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCT1845710.10977765746466005No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGA1818450.10815630907163698No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGCC1777620.1057278551139285No Hit
AGGTCGGCTCTGGCGGGTGCGTGGGGGGCG1763370.10488030505521322No Hit
GGGGCGGTAGCTCAGCCGGTTAGAGCAGC1750960.10414219303916714No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGGT1709130.10165426188435585No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)