FastQCFastQC Report
Thu 29 Jun 2023
ERR3949752_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949752_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170844372
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGTAGTTTAATGGTAGAACATGAG3473550320.331663603176814No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCAAC118077126.9113848245466345No Hit
GCACTCGTAGCTTAACGGATAGAGCATCT75187464.400932797481909No Hit
TGGCCTATGGTGTAATTGGCAGCACGACT56411723.301936103578525No Hit
GGGGCGGTAGCTCAGCCGGTTAGAGCAGCG39295682.300086303106315No Hit
GCCCCAATAGCTCAGTCGGTAGAGCGTCTC33294641.9488286099351284No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCA29341181.7174215138910165No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGG28089201.6441396149707526No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGG27823861.6286085209760377No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGC20381431.192982230635025No Hit
GTCCGGGTGGCGGAATGGCAGACGCGCTAG19880711.163673685428748No Hit
GCGGGTGTAGTTTAATGGTAGAACAT12658090.7409134905538475No Hit
GGTGGGTGTAGCTCAGCTGGTAGAG12634260.7395186538541638No Hit
GGTCCTGTGGAGCAGTTTGGAGTGCTCGCC12559770.7351585453455851No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAGC11337630.6636232652720923No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTG10640560.6228218041622114No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCG9930380.5812529780026936No Hit
AGCGGTGTAGCTCAGTCGGTAGAGCAAGCG9759130.5712292354588069No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCC9198250.538399356813463No Hit
TTCTGGCGAAGCTGAGAACTTAAGCGC9104400.5329060532353972No Hit
GCGGACGTAGCTCAGTTGGTAGAGCG8640710.5057649777307268No Hit
CGGATCTCTGGGCCATTACTGACGCTGAGGAGC6465080.3784192551569682No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTGGT6266580.3668004937265361No Hit
GTCCGGGTGGCGGAATGGCAGACGCGC5638310.3300260894751628No Hit
GCGGGTGTAGTTTAATGGTAGAACATG5074860.2970457815256566No Hit
GCGGACGTAGCTCAGTTGG4948390.28964313790799034No Hit
GCCGGTGTAGTTTAATGGTAGAACATGAG4539980.2657377557628881No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTGG3860500.22596588666087286No Hit
GGGTGCGTAGCTCAGGGGTAGAGCGCTG3827890.22405713194930413No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCC3501470.20495085433660057No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCC3460950.20257910515190983No Hit
CGGGACGTGGCGCAGCTTGGTAGCGCACTTG3331460.19499969246865212No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACT3302740.19331863036143795No Hit
AGGGTCGTAGCTCAATTGGTAGAGCAC3285200.19229196499373125No Hit
AGCGGTGTAGCTCAGTCGGTAGAGCAAGC3249110.19017951612711012No Hit
CGCGGGGTGGAGCAGCTCGG3240000.18964628229017694No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCT3238220.1895420939005237No Hit
ACTCGTAGCTTAACGGATAGAGCATCT3162730.18512345258876892No Hit
TGGTGGAGCCGACGACCCGAGGTG3116270.18240401855321287No Hit
AGCGGTGTAGCTCAGTCGGTAGAG2913280.17052244483652057No Hit
GCGCGTGTAGTTTAATGGTAGAACATGAG2880370.16859613028399906No Hit
TTCTGGCGAAGCTGAGAACTTAAGC2783940.16295181207373924No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGG2768960.16207499068216308No Hit
ACGTCACGAAAGTCGGTAACACCC2707820.15849629509598362No Hit
ACCGTTGGACGCGGGTTCGATTCCCGCCATCTC2703390.15823699477791403No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTG2611210.15284144098115213No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGC2443960.1430518296499694No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGA2393380.14009124046532828No Hit
ACCGTTGGACGCGGGTTCGTTTCCCGCCATCTC2366970.13854538913345066No Hit
TCCCCTGTAGCTCAATTGGCAGAGCATTCG2354950.13784182483927537No Hit
GGGGCTATAGCTCAGTTGGTAGAGCGCTTG2341540.13705689994868545No Hit
CGGCTTGTCACGTCGGGTGTGAAAGCCCGGGG2267790.1327401057144569No Hit
GCCCCAATAGCTCAGTCGGTAGAGCGTCT2264070.13252236368664225No Hit
TCGCAGGTCGTCGGTTCGTTTCCGACAGGCGG2263240.13247378146000618No Hit
GGGCCTGTGGCGCAGTCCGGTAGCGCACCTC2236260.13089456643031822No Hit
GCCGACGTAGCTCAGTTGGTAGAGCGCAAC2225770.13028055732500218No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAT2218700.129866730406548No Hit
ACCGGGACGGACGAACCTCTGGTGTGCCAG2214110.12959806483997027No Hit
GCCCTCGTAGCTTAACGGATAGAGCATCT2148230.12574192376673665No Hit
GCAATCGTAGCTTAACGGATAGAGCATCT2131450.1247597433294437No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCT2107780.12337427187826826No Hit
GGGGCGGTAGCTCAGCCGGTTAGAGCAGC2005920.11741212054676288No Hit
AGGTCGGCTCTGGCGGGTGCGTGGGGGGCG1996730.11687420408557563No Hit
GCGGGTGTAGTTTAATGGTAGAACATGGG1991050.11654173776353605No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGC1961650.11482087335016222No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGG1946500.11393410138204611No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGGT1917780.11225303927483196No Hit
GCGGACGTAGCTCAGTTGGTAGAGC1915880.11214182694879758No Hit
TGGACTATGGTGTAATTGGCAGCACGACT1896850.11102794770435867No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGCC1878490.10995328543804768No Hit
TGGCCTATGGTGTAATTGGCAGCACGAC1864440.10913089955342514No Hit
GGGGATGATCGGTTTCGACAGCG1828050.10700089084585121No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTT1812060.10606495132306729No Hit
AACGTTGGGAACTAGGTGTTGGCG1749900.10242655227764835No Hit
ATGCGGGGTTGTGGGATCTCTCTTTCACAGT1745450.10216608130351522No Hit
TCCCCTGTAGCTCAATTGGCAGAG1723280.10086840905710373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)