FastQCFastQC Report
Sat 5 Aug 2023
ERR3949753.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949753.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115968327
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGACGTGGCTCAGTTGGTAGAGCATCAC1750170215.091794848433054No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCC67544795.824417041042594No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCG26871032.3171007718340197No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGA26480822.2834527913815643No Hit
TGGCCTATGGTGTAATTGGCAGCACGACG26150392.2549596667027885No Hit
GGGCCTGTGGCGCAGTCTGGTAGCGCACCTC19925221.718160511188542No Hit
TGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGCTT17822891.5368756677847046No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTCG11860161.0227068292534736No Hit
AGGAGAAGGTCAACGGTTCGTTTCCGTTAAAGGG11712671.0099887014839837No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTC11552440.9961719978938733No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA10586830.9129070215870234No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGG10237610.8827936269184946No Hit
ATGCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCG10022060.8642066553223624No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGGG9729080.8389428606657402No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGC9273700.7996752423616493No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATC8890170.7666032812562692No Hit
AGGAGAAGGTCAACGGTTCGTTTCCGTTAAAGG8563620.7384447306892683No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACCG8354020.7203708302181508No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA7948910.6854380161921281No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACT7771150.6701096929681498No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCC7597200.6551099077250636No Hit
GTCCGGGTGGCGGAATGGCAGACGCGCTAG7218350.6224415050844012No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACTC7109290.61303721316942No Hit
TCCGCTGTGCGCGTAGGAATATTGAGAAGGGCTGTCC6697390.5775188944477917No Hit
GCCCTTTTAGCTCAGTCGGTAGAGCGTCTC6449340.556129433513342No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCT6441390.5554439015059689No Hit
GCCCCAATAGCTCAGTCGGTAGAGCGTCTC6411150.5528362929647161No Hit
ACCGTATGGATAGGCGAAGGACATGCGAAAGGTCCGG5745320.49542147831450567No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAAC5637990.48616636506276406No Hit
GGGGCGGTAGCTCAGCCGGTTAGAGCAGCG5347200.461091415072324No Hit
ATGCAGGAGGTCAGGAGTTCATTTCTCCTTAGCTC5072650.43741684744663084No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAAC4718280.40685936600603023No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGG4579760.39491472529391586No Hit
ATGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGCTT4272180.36839196619608044No Hit
ATGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGC3820450.32943908900229285No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATCACC3781110.32604678344631116No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCC3683860.31766087304165386No Hit
TCCAGGATGTCGCGGGTTCGAGTCCCGTTACTCACCC3672260.3166605999239775No Hit
TGCAGGAGGTCAGGAGTTCATTTCTCCTTAGCTC3614190.31165319820471327No Hit
GAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGC3600230.3104494212458545No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACT3509200.3025998641853306No Hit
TGGATGATCCTATGCTGTCGAGAAAAGC3406610.2937534832247774No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAAC3336740.28772856229960103No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGG3272490.28218825645385054No Hit
GGAGAAGGTCAACGGTTCGTTTCCGTTAAAGGG3175770.2738480481830181No Hit
ACAAGCAGCGGAGCATGTGGCTTAATTC3076970.26532848059453334No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCT3009050.259471709029656No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTGCAG2937390.25329243561476916No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACT2900840.25014071298967694No Hit
GCGGACGTGGCTCAGTTGGTAGAGCAT2854080.24610857756014712No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCG2783500.2400224330217336No Hit
ACTGGGTTGATAGGCCGGATGTGGAAGCC2495400.21517944291806504No Hit
ACCGTTGGACGCGGGTTCGATTCCCGCCATCTC2494120.2150690679533559No Hit
GGAGAAGGTCTACGGTTCGTTTCCGTATTGGG2465180.2125735589856358No Hit
GCACTCGTAGCTTAACGGATAGAGCATCT2399790.2069349504369413No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGT2378210.20507409751629857No Hit
GGAGAAGGTCAACGGTTCGTTTCCGTTAAAGG2354790.20305458058388648No Hit
TCCCCTGTAGCTCAATTGGCAGAGCATTCG2291780.19762120048519802No Hit
ATCCGCTGTGCGCGTAGGAATATTGAGAAGGGCTGTCC2291200.19757118682931415No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTG2270660.1958000135674976No Hit
TTCCGTTAGTCTTGGTTCGAGTCCAGGTAGGC2269860.19573102921455443No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTGC2207260.19033300359674932No Hit
AAACCGGATGAGGCCAAACCGTATGC2199630.1896750653305536No Hit
ATGGATAGGCGAAGGACATGCGAAAGGTCCGGCG2097070.1808312712832358No Hit
AGGGTTCCTGGGTCAAGCTGATCTGCCCAGGGTAAGTCG2096220.18075797540823366No Hit
GGAGAAGGTCTACGGTTCGTTTCCGTATTGGGG2095230.18067260727146647No Hit
TGCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCG2089380.18016815919056933No Hit
AGGAGAAGGTCAACGGTTCGTTTCCGTTAAAG1982620.1709621972902998No Hit
TCCAGGATGTCGCGGGTTCGAGTCCCGTTACTCACC1982160.17092253128735746No Hit
ACCGTATGGATAGGCGAAGGACATGCGAAAGGTC1962400.16921861776966052No Hit
ACTACGAGTAAAGATGCTCGTTTCGCGCAGCAGGAC1943390.16757937708284779No Hit
AGGAGAAGGTCAACGGTTCGATTCCGTTAAAGGG1932560.16664550140487927No Hit
GGGGCTATAGCTCAGTTGGTTAGAGCG1916830.1652890965651337No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCG1865230.16083960580029752No Hit
ACCGGGACGGACGAACCTCTGGTGTGCCAG1854830.15994280921203596No Hit
GGCTCTGTGGAGCAGTTGGTTAGCTCGCC1844650.1590649833208338No Hit
AGGGCGATTTAGTAGCGCGCTCAAGACCC1801350.15533120521778332No Hit
TGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGC1762240.1519587326632728No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTC1723980.14865955598376443No Hit
ACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACTC1714530.14784467831462292No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCT1701810.1467478271028261No Hit
AACCGTATGGATAGGCGAAGGACATG1696780.14631408798369575No Hit
AGGAGAAGGTCTACGGTTCGATTCCGTATTGGG1682200.145056848151306No Hit
GAAAGGTCCGGCGTAGAGGGTAAGACCCCCG1633150.14082724501147628No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTGCAG1598090.13780400574374072No Hit
ATGGGTGGAGCTGACCGGTACTAATAGGCCG1569700.13535592351866904No Hit
AACCGTATGGATAGGCGAAGGACATGCGAAAGGTCCGG1522030.13124531838766632No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTG1513100.1304752805479379No Hit
AGACCCCGGGACCTTTACTACAGTTTGATATTGG1478580.12749860571843896No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGC1478370.12748049732579136No Hit
AGGGTCGTAGCTCAATTGGTAGAGC1471990.1269303471110694No Hit
CGGGACGTGGCGCAGCTTGGTAGCGCACTTG1447870.12485046886983203No Hit
AGCGGGTGTCGCAGGTTCGAATCCTGCCGGGGG1439330.12411406090216341No Hit
AGGAGAAGGTCAACGGTTCGATTCCGTTAAAGG1431780.12346302107126199No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCC1408740.12147627170649793No Hit
AGACCACTGGGTTGATAGGCCGGATGTGGAAGCC1399640.12069157469176907No Hit
GTTTCTTGCCGGAGGTAGAGCACTGGATAG1388600.11973959062115296No Hit
AAGCGGGGGTCGTCGGTTCGAACCCGACAGGGGG1380890.1190747539196629No Hit
AGGAGAAGGTCTACGGTTCGATTCCGTATTGGGG1378030.11882813485789098No Hit
ACCGAGGCGTTCGGGTGAACTATGGTTAAGGA1366100.11779940569462558No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATCGC1302210.11229014280769954No Hit
TCCCCTGTAGCTCAATTGGCAGAG1299870.1120883635753407No Hit
TACCGAGGCGTTCGGGTGAACTATGGTTAAGGA1294150.11159512545179685No Hit
GCGGACGTGGCTCAGTTGGTAGAGCGTCTC1258950.1085598139222962No Hit
CGGATCTCTGGGCCATTACTGACGCTG1244260.10729308874137676No Hit
GGACGATTAGCTCAGCGGGAGAGCACTTC1216540.10490278091189502No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)