FastQCFastQC Report
Sat 5 Aug 2023
ERR3949754.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949754.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13034557
Total Bases349.9 Mbp
Sequences flagged as poor quality0
Sequence length15-51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGACGTGGCTCAGTTGGTAGAGCATCAC8306166.37241449786134No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCC3249412.492919398795064No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGG1983001.5213405411476584No Hit
GCGGACGTGGCTCAGTTGGTAGAGCAT1802331.3827320713699744No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCG1514541.1619420590972136No Hit
GAAGAGGTCGTGGGTT1477181.1332797884884005No Hit
ACGAAGAGGTCGTGGGTT1470911.1284694984263755No Hit
AGGATGAGGCCACAGGTT1456441.1173682389052424No Hit
CTCAGTTGGTAGAGCATCAC1210160.9284243415407213No Hit
AGGAGGTCAGGAGTT1137320.8725421201503051No Hit
TGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGCTT1020240.7827193513366048No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAG994270.7627953907447718No Hit
TGGCCTATGGTGTAATTGGCAGCACGACG840960.6451772776013792No Hit
CCCAGGAGCTCCAATAA712680.5467619651362144No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGA663030.508670912252714No Hit
GGGCCTGTGGCGCAGTCTGGTAGCGCACCTC649820.4985363138923709No Hit
GGCGGTAGCACGGGTTCGAGTCCCGTTGGGGG641790.4923757669708299No Hit
ATGCAGGAGGTCAGGAGTT598640.45927145817076864No Hit
GCCCCAATAGCTCAGTCGGTAGAGCGTCTC580350.4452395275113684No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTCG553210.4244179529845165No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATC545610.41858729836388No Hit
AGGAGAAGGTCAACGGTTCGTTTCCGTTAAAGGG501500.3847464858222646No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCG478760.3673005534442022No Hit
TATGCTGTCGAGAAAAGCC471710.3618918540921644No Hit
GCCCTTTTAGCTCAGTCGGTAGAGCGTCTC451570.3464406193474776No Hit
AGGTGGGAGGCTTTGAAGTGTGGACGC430560.33032192808700744No Hit
AGCTCAGTCGGTAGAGCGTCTC430190.33003806726995016No Hit
GGGGCTATAGCTCAGTTGGTTAGAGCG426600.32728385015309686No Hit
AATTGGCAGCACGACG401740.30821147201243587No Hit
AGGCGTTCGGGTGAAC396700.3043448273692769No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTC386200.29628931769602906No Hit
TCCCCTGTAGCTCAATTGG366750.28136744501558436No Hit
GCGGGTGTAGTTCAG359890.27610451202906244No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGGG354370.27186961551512645No Hit
TGGATGATCCTATGCTGTCGAGAAAAG345320.2649265333681843No Hit
GAAGAGGTCGTGGGT344860.2645736253253563No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGC334820.2568710236949365No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGG332260.2549070137174589No Hit
TGGAGGTCGCGGGTT328940.2523599382779177No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGG328550.25206073363291137No Hit
TGCAGGAGGTCAGGAGTT320430.24583113948559973No Hit
ATAGCTCAGTTGGTAGAGCGCCTG317990.24395919247581643No Hit
AGGAGAAGGTCAACGGTTCGTTTCCGTTAAAGG316350.24270099858399483No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCC315970.242409465852963No Hit
ATGCAGGAGGTCAGGAGTTCATTTCTCCTTAGCTC303810.23308041845994462No Hit
CGCGGGGTGGAGCAGCT301650.2314232850414479No Hit
ATTGAGAAGGGCTGTCC298070.22867673983856912No Hit
GGGGCGGTAGCTCAGCCGGTTAGAGCAGCG296780.2276870629358558No Hit
AGCAGCTCGGTAGCTCGCTGG288730.22151117218636585No Hit
ACGAAGAGGTCGTGGGT287430.220513823369678No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGC280300.21504374870584403No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTG278870.2139466650074874No Hit
ATGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGCTT278790.21388528969569126No Hit
GTCCGGGTGGCGGAATGGCAGACGCGCTAG267250.20503190096909318No Hit
CGGAAGAGGTCACTGGTT265210.2034668305182907No Hit
GCGGACGTGGCTCAGTTGG263350.20203985451902967No Hit
CTCAGTTGGTAGAGCAT260170.19960018587513179No Hit
ACCCAGAGGTCGCAGGTT259920.19940838802576874No Hit
TGCAGGAGGTCAGGAGTTCATTTCTCCTTAGCTC256440.19673856196263517No Hit
TGGAGGTCGCGGGTTCAGGTCCCGT256190.19654676411327213No Hit
ACCGGGACGGACGAACCTCTGGTGTGCCAG251960.19330154450204945No Hit
AGGTGGGAGGCTTTGAAGTGTGGACG246320.18897458502041917No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCT242070.18571402158124745No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAG237880.18249948962592286No Hit
AGGCGTTCGGGTGAACTATGGT235750.18086537194934973No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGG230660.17696036773631815No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACT227460.1745053552644712No Hit
CTCAATTGGCAGAGCAGCCG222760.170899555696446No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCG221520.16994823836360531No Hit
AGGTGAGGGTCGCGAGTT219720.16856729384819139No Hit
GAGAGGACCGGAGTGGACG218790.16785380584856088No Hit
GCGGACGTGGCTCAGTTGGTAGAGC212540.1630588596144848No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA208210.15973692086351687No Hit
TCCGCTGTGCGCGTAGGAATATTGAGAAGGGCTGTCC206450.15838666400400106No Hit
AGCTCAGTTGGTAGAGCGCCTG205840.15791867725155523No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCC205080.15733561178949157No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGGT203420.15606207406972097No Hit
GCGGGTGTAGTTCAGTAG201850.15485758357572105No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCT198130.15200363157719898No Hit
AATAGCTCAGTCGGTAGAGCGTCTC192510.14769201592351777No Hit
GCGGACGTGACTCAGTTGGTAGAGCATCAC192050.14733910788068977No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTG191580.14697852792388724No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACCG190370.14605022633297013No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACTC187260.14366426108689387No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGC181370.13914550375590057No Hit
TGGAGCTGACCGGTAC180930.1388079395410216No Hit
CGGGGTGTAGCGCAGCTTGG177590.13624552027353137No Hit
ATGCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCG173280.1329389253505125No Hit
AGGATAGGTGGGAGGCTTTGAAGTGTGGACGC172360.13223310926485649No Hit
CGCGGGGTGGAGCAG170570.1308598366634171No Hit
CGGATCTCTGGGCCATTACTGACGCTGAGGAGC168260.12908762453530256No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGT168240.12907228070735355No Hit
GCGGACGTAGCTCAGTTGGTAGAGCGCAAC167620.1285966220409332No Hit
GCGGACGTGGCTCAGTTGGTAGAG166880.12802890040681858No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAAC164430.12614928148306076No Hit
ACGGAAGAGGTCACTGGTT163110.1251365888384239No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGGTACAA161850.12416992767763416No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA160850.12340273628018197No Hit
AAGCGTACACGGTGGATGCCCTGG158720.12176861860360887No Hit
CTCAGTCGGTAGAGCGTCTC157900.12113952165769806No Hit
GATGTCGACTTGGAGGTTGTGCCCTTGAGGCGTGGCTTCCGGAGC157890.12113184974372355No Hit
AACCGGCGGTTGGGGGTT156980.12043370557204207No Hit
AAGGCGATGTATACGG155040.11894535426098486No Hit
TCCCCTGTAGCTCAATTGGCAGAG153420.11770250419711234No Hit
ACCGATAGGCTGTAGGTTCGAATCCTACCTGGG151960.11658240475683217No Hit
ACCGGCGGTTGGGGGTT149900.11500199047808068No Hit
ACTGCGAAGGGGGGACGGAGAAGGC149200.11446495649986416No Hit
ATGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGC148970.11428850247845017No Hit
TGGATGATCCTATGCTGTCGAGAAAAGC148600.11400464166139287No Hit
TGACCGGTACTAATAGGCCGAGGGCTT147660.11328348174778782No Hit
TTTAATGGTAGAACATGAG146180.1121480384795586No Hit
GAGGCGTGGCTTCCGGAG144570.1109128603296606No Hit
CGGAAGGGGTCACTGGTT138970.10661658850392845No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATCACC136610.10480601680594132No Hit
GAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGC135580.10401580966656557No Hit
GGCTCTGTGGAGCAGTTGGTTAGCTCGCC135490.10394676244079488No Hit
AACTGGAGGACCGAACCGAC134490.1031795710433427No Hit
AGTTGGTAGAGCGCCTG133820.10266555280704975No Hit
CGCGGGGTGGAGCAGCTCGG132930.10198275246331731No Hit
TGGGTGGAGCTGACCGGTAC132770.10186000183972498No Hit
AGGTGGAGGTCGCGGGTTCAGGTCCCGT132650.10176793887203071No Hit
AGGTGGGAGGCTTTGAAG132570.10170656356023454No Hit
ACCGTTGGACGCGGGTTCGATTCCCGCCATCTC131440.10083963728111359No Hit
AATGTCTGGGAAACTGCCTGATGGAGGGGG130430.10006477396968688No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)