FastQCFastQC Report
Sat 5 Aug 2023
ERR3949755.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949755.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95132868
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCC1317826913.852487869912636No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATCAC85352118.971884459532955No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCC28013232.944642644432837No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCG20937122.200829265443779No Hit
TGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGCTT18776691.973733200180615No Hit
TGGCCTATGGTGTAATTGGCAGCACGACG16376541.72143869351232No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCT15108641.5881619378909084No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGA13673991.4373570656988919No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCT11124341.1693476958983304No Hit
GGGCCTGTGGCGCAGTCTGGTAGCGCACCTC10978431.1540101997135206No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGC9625341.0117786000102509No Hit
GCCCCAATAGCTCAGTCGGTAGAGCGTCTC9345990.982414405923303No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA8671930.9115598196829302No Hit
GGGGCGGTAGCTCAGCCGGTTAGAGCAGCG8670500.9114095036007954No Hit
GCCCTTTTAGCTCAGTCGGTAGAGCGTCTC8046960.8458653848215739No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACTC8012730.8422672593030623No Hit
ATGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGCTT7748070.8144472213325893No Hit
TCCGCTGTGCGCGTAGGAATATTGAGAAGGGCTGTCC7540010.7925767569626935No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAAC6413750.6741886516025145No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA6399250.6726644675528967No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCC6118850.6431899015175281No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATC5366030.5640563679841966No Hit
GTCCGGGTGGCGGAATGGCAGACGCGCTAG5337550.5610626602784644No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTCG5261140.5530307359176851No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCC4854690.5103062802647766No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGG4739600.4982084635564651No Hit
TGGATGATCCTATGCTGTCGAGAAAAGC4595520.48306332991033135No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGG4513510.47444275515797546No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGG4492700.47225528825642044No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGGG4478290.4707405646595244No Hit
ATGCAGGAGGTCAGGAGTTCATTTCTCCTTAGCTC4356280.45791534425305036No Hit
AGGAGAAGGTCAACGGTTCGTTTCCGTTAAAGGG4340690.4562765836093578No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTC4315640.45364342426846627No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCG4210730.4426156898791278No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACT4208000.44232872281323427No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGC4194990.4409611618142323No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAAC3629060.38147278393835454No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACT3432170.3607764668673712No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCT3406790.3581086191998332No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACT3403920.35780693587415024No Hit
AGGAGAAGGTCAACGGTTCGTTTCCGTTAAAGG3343630.35146948371198056No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTG3157420.33189580703064686No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTG3136900.32973882381008424No Hit
ATGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGC3051650.32077767275974484No Hit
ATCCGCTGTGCGCGTAGGAATATTGAGAAGGGCTGTCC3051050.32071460307493305No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGGTACAA2944990.3095659851230387No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACCG2926180.30758875050418955No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCG2852760.29987112340605565No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTGCAG2845070.29906278027905137No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTGCAG2796280.29393416374244075No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTGC2732370.2872161911485734No Hit
ATGCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCG2611670.27452867288727173No Hit
AGACCCCGGGACCTTTACTACAGTTTGATATTGG2588700.27211415512039433No Hit
TGCAGGAGGTCAGGAGTTCATTTCTCCTTAGCTC2568200.26995927422265875No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCC2392900.2515324146434858No Hit
GCACTCGTAGCTTAACGGATAGAGCATCT2283040.23998435535445015No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATCACC2092280.2199324002299605No Hit
ACCGTATGGATAGGCGAAGGACATGCGAAAGGT2066350.21720673868467835No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAAC2050320.2155217269387905No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGT1997090.20992639473457272No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCT1927500.20261136245782055No Hit
ACCGGGACGGACGAACCTCTGGTGTGCCAG1905550.2003040631551232No Hit
ACCGTTGGACGCGGGTTCGATTCCCGCCATCTC1861320.195654776223082No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGGT1846330.19407908526420123No Hit
TCCCCTGTAGCTCAATTGGCAGAGCATTCG1702540.17896443529905984No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGC1656840.17416062763922982No Hit
AACCGTATGGATAGGCGAAGGACATGCGAAAGGT1610320.16927062474349033No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTC1599760.16816059829080313No Hit
TCCCCTGTAGCTCAATTGGCAGAG1537030.16156666274373227No Hit
GATGATCCTATGCTGTCGAGAAAAGCC1484590.15605437229118332No Hit
GGGGCTATAGCTCAGTTGGTTAGAGCGC1464820.1539762261766354No Hit
TGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGC1457820.15324041318716472No Hit
CGGATCTCTGGGCCATTACTGACGCTGAGGAGC1456260.1530764320066541No Hit
GTTTCTTGCCGGAGGTAGAGCACTGGATAG1418160.14907150702110653No Hit
ATGGGTGGAGCTGACCGGTACTAATAGGCCG1342520.141120522089169No Hit
ACCGTTGGACGCGGGTTCGTTTCCCGCCATCTC1332850.14010404900228593No Hit
TCCAGGATGTCGCGGGTTCGAGTCCCGTTACTCACCC1330150.13982023542063296No Hit
TCGGGGTGATGGGGACTCACAGGAGACCGCCGGG1293990.13601923574931013No Hit
AGGGCGATTTAGTAGCGCGCTCAAGACCC1259910.1324368776520014No Hit
AGGAGAAGGTCAACGGTTCGTTTCCGTTAAAG1195160.12563060749939758No Hit
GCGGACGTGGCTCAGTTGGTAGAGCAT1189830.12507033846598634No Hit
GGCTCTGTGGAGCAGTTGGTTAGCTCGCC1186690.12474027378213805No Hit
GAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGC1185610.12462674834947686No Hit
GGAGAAGGTCTACGGTTCGTTTCCGTATTGGG1147220.12059133968293692No Hit
AGGGTTCCTGGGTCAAGCTGATCTGCCCAGGGTAAGTCG1119680.11769644115007655No Hit
AAACCGGATGAGGCCAAACCGTATGC1105530.11620904774993224No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTC1065080.1119570998322052No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACTC1001480.10527171324215727No Hit
GGAGAAGGTCAACGGTTCGTTTCCGTTAAAGGG987040.10375383616102062No Hit
ACCGTATGGATAGGCGAAGGACATGCGAAAGGTCCGG986570.10370443157458473No Hit
TCCGCTGTGCGCGTAGGAATATTGAGAAGGG982550.10328186468634584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)