FastQCFastQC Report
Sun 2 Jul 2023
ERR3949756.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949756.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157601248
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGACGTGGCTCAGTTGGTAGAGCATCAC1879719611.927060374547288No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCC1687729610.708859361316733No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCC52946983.359553345668938No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCT33894512.150649847645876No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCT30524061.9367905005422292No Hit
GGGGCGGTAGCTCAGCCGGTTAGAGCAGCG23531691.4931157144136322No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCG22817491.4477988143850231No Hit
TGGCCTATGGTGTAATTGGCAGCACGACG16095221.0212622174159434No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGG15700950.9962452835398866No Hit
GGGCCTGTGGCGCAGTCTGGTAGCGCACCTC15555090.9869902806861022No Hit
GCACTCGTAGCTTAACGGATAGAGCATCT14155820.8982048162461252No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATC13175850.8360244710752545No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGG12930300.8204440106971742No Hit
GTCCGGGTGGCGGAATGGCAGACGCGCTAG11361740.7209168800490717No Hit
GCCCCAATAGCTCAGTCGGTAGAGCGTCTC10152150.6441668532980145No Hit
GCCCTTTTAGCTCAGTCGGTAGAGCGTCTC9935680.6304315559734655No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGGG9053350.5744465932147949No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGC8699100.5519689793319402No Hit
ATGCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCG8263550.5243327768571985No Hit
AGGAGAAGGTCAACGGTTCGTTTCCGTTAAAGGG7450990.4727748094989705No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCT6642400.42146874369928844No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTG6473610.4107588031282595No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCC6430970.4080532407966719No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGA6238200.3958217386704958No Hit
GCGGCCGTGGCTCAGTTGGTAGAGCATCAC6039360.3832050873099685No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCC5922400.37578382628036044No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGC5755260.3651785803117498No Hit
ATGCAGGAGGTCAGGAGTTCATTTCTCCTTAGCTC5186230.3290729017577323No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGG4688470.2974893955154467No Hit
TGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGCTT4535010.2877521629777957No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCT4523850.2870440467578023No Hit
GCGGAAGTGGCTCAGTTGGTAGAGCATCAC4473160.2838277016689614No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTGC4438590.28163419112011095No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTC3913440.2483127544776803No Hit
TGCAGGAGGTCAGGAGTTCATTTCTCCTTAGCTC3761030.23864214577793189No Hit
GGGGCTATAGCTCAGTTGGTTAGAGCGC3392100.2152330671899248No Hit
AGGAGAAGGTCAACGGTTCGTTTCCGTTAAAGG3379900.2144589616447707No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTGCAG3330520.2113257377251226No Hit
GGTAGCGTGTCCGAGCGGCCGAAGGAGCTCG3319640.21063538786190322No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATCACC3200220.20305803669778047No Hit
TGGATGATCCTATGCTGTCGAGAAAAGC3161050.20057265028764237No Hit
ATGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGCTT3110250.19734932555864027No Hit
TCCCCTGTAGCTCAATTGGCAGAGCATTCG2972540.18861145058952833No Hit
TCCAGGATGTCGCGGGTTCGAGTCCCGTTACTCACCC2883410.18295603852071018No Hit
TGGTGAAGTGGATGATCCTATGCTGTCGAGAAAAGCC2803690.17789770294204776No Hit
CGGATCTCTGGGCCATTACTGACGCTGAGGAGC2774510.1760461947610973No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACCG2731360.17330827227967127No Hit
GCGGACGTGGCTCAGTTGGTAGAGCAT2656930.1685855939414896No Hit
TAGGACGTGGCTCAGTTGGTAGAGCATCAC2526560.16031345132495398No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGGT2451150.1555285907380632No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGC2431220.15426400684339758No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTGCAG2380080.15101910868116983No Hit
TCGGACGTGGCTCAGTTGGTAGAGCATCAC2322720.14737954359346192No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCG2314830.1468789130400795No Hit
ACCGTATGGATAGGCGAAGGACATGCGAAAGGTCCGG2301760.14604960488637755No Hit
ACCGTTGGACGCGGGTTCGATTCCCGCCATCTC2260220.1434138389564022No Hit
GGCTCTGTGGAGCAGTTGGTTAGCTCGCC2238110.14201093128399592No Hit
GAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGC2115860.13425401301390708No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTCG2110930.13394119823213585No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGT2058120.13059033644200582No Hit
TCCGCTGTGCGCGTAGGAATATTGAGAAGGGCTGTCC1985200.1259634695278555No Hit
GAGGCCGTGGCTCAGTTGGTAGAGCATCAC1958060.1242414019462587No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCC1953770.12396919597997091No Hit
TCCCCTGTAGCTCAATTGGCAGAG1920320.12184675085821656No Hit
ACGGGGTCTAATACCGGATAACACCGGC1904340.12083279949661313No Hit
GGACGATTAGCTCAGCGGGAGAGCACTTCC1892220.1200637700533945No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTG1873490.11887532768776045No Hit
AAGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGT1863190.11822177956357299No Hit
TAGTCCGTGGCTCAGTTGGTAGAGCATCAC1854380.11766277383793305No Hit
TGGGTGATCCTATGCTGTCGAGAAAAGCC1840110.11675732415519957No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCG1838430.11665072601455542No Hit
ACCGGGACGGACGAACCTCTGGTGTGCCAG1794520.11386458056474275No Hit
TGGATGATCCTATGCTGTCGAGAAAAG1773210.11251243391169086No Hit
GAGGACGTGGCTCAGTTGGTAGAGCATCAC1749550.11101117676428553No Hit
AACCGTATGGATAGGCGAAGGACATGCGAAAGGT1732720.10994329181961808No Hit
GCGTCCGTGGCTCAGTTGGTAGAGCATCAC1726620.10955623904704105No Hit
GCGTACGTGGCTCAGTTGGTAGAGCATCAC1712130.10863683008398511No Hit
TAGGCCGTGGCTCAGTTGGTAGAGCATCAC1697350.10769902025141323No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGGTACAA1628330.10331961330661543No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)