FastQCFastQC Report
Sat 5 Aug 2023
ERR3949759.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949759.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107315300
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCC1146079210.67955081894194No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATCAC1133597010.563237488037586No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCC24628982.295011056205406No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCG23879032.225128197004528No Hit
TGGCCTATGGTGTAATTGGCAGCACGACG21277531.9827116916227228No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGG18089541.6856440787101186No Hit
GGGCCTGTGGCGCAGTCTGGTAGCGCACCTC17629401.6427666884405112No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCT17106871.5940755884762003No Hit
TGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGCTT16066161.497098736154118No Hit
GCCCCAATAGCTCAGTCGGTAGAGCGTCTC14057151.3098924384500625No Hit
ATGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGCTT13563081.263853336849452No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGA13281551.2376194261209725No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGC12286311.1448796210791936No Hit
GGGGCGGTAGCTCAGCCGGTTAGAGCAGCG12070231.1247445611203621No Hit
GCCCTTTTAGCTCAGTCGGTAGAGCGTCTC11625911.0833413315715466No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGG11310011.053904708834621No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCT11149751.0389711439095823No Hit
GTCCGGGTGGCGGAATGGCAGACGCGCTAG9684190.9024053420155375No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACTC8997140.8383837160218532No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCC8206370.7646971121545576No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTCG7616220.7097049535341187No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGGG7266640.677129915305646No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA6700950.6244170216176073No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAAC6640440.6187784966356149No Hit
TCCGCTGTGCGCGTAGGAATATTGAGAAGGGCTGTCC6537080.6091470647708203No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTG6488710.6046397857528237No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTC6294140.5865090998208083No Hit
ATGCAGGAGGTCAGGAGTTCATTTCTCCTTAGCTC6293370.5864373486352831No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACT5553250.517470481841825No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATCACC5426620.5056706732404419No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGC5395710.5027903756500705No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCC5307080.49453153464603833No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCT5228040.4871663220435483No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACT5067950.4722485982893399No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA4945360.46082525045357No Hit
AGGAGAAGGTCAACGGTTCGTTTCCGTTAAAGGG4884610.45516436146569966No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGGTACAA4676270.43575054069643376No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATC4563080.4252031164242191No Hit
TGGATGATCCTATGCTGTCGAGAAAAGC4159150.3875635626979564No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAAC4017810.3743930269029673No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGG3945260.3676325742927616No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCG3814550.3554525775914525No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCG3812590.35526993821011543No Hit
ATGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGC3762220.3505762924764689No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACT3575890.3332134374129318No Hit
TGCAGGAGGTCAGGAGTTCATTTCTCCTTAGCTC3531570.3290835509941267No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAAC3176380.29598575412825573No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACCG2987620.2783964635052038No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTG2985720.27821941512533627No Hit
ATCCGCTGTGCGCGTAGGAATATTGAGAAGGGCTGTCC2773000.25839745124879676No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTGCAG2760710.2572522277811272No Hit
ATGCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCG2686540.25034081813124504No Hit
GGGGCTATAGCTCAGTTGGTTAGAGCGC2660710.24793389199862462No Hit
AGACCCCGGGACCTTTACTACAGTTTGATATTGG2659760.24784536780869085No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCT2531990.23593933017938729No Hit
AGGAGAAGGTCAACGGTTCGTTTCCGTTAAAGG2441910.22754537330650892No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACTC2411670.22472750856588017No Hit
ACCGTATGGATAGGCGAAGGACATGCGAAAGGT2273300.21183372734363132No Hit
TGCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGC2213800.20628931755304228No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGC2056660.19164648470441772No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTGCAG2025370.18873077743807265No Hit
AACCGTATGGATAGGCGAAGGACATGCGAAAGGT2006830.1870031579839967No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCC1985950.18505748947261016No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTC1974310.18397283518752683No Hit
ACCGTTGGACGCGGGTTCGATTCCCGCCATCTC1950360.1817410937676175No Hit
CGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGC1862180.1735241852746067No Hit
ATGGGTGGAGCTGACCGGTACTAATAGGCCG1825190.170077332868659No Hit
TCCCCTGTAGCTCAATTGGCAGAG1812320.1688780630534509No Hit
ACCGGGACGGACGAACCTCTGGTGTGCCAG1791740.16696034954941188No Hit
GAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGC1770820.16501095370371233No Hit
GGACGATTAGCTCAGCGGGAGAGCACTTCC1755780.16360947600202394No Hit
GGCTCTGTGGAGCAGTTGGTTAGCTCGCC1714300.15974423031944188No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGGT1703250.1587145542154753No Hit
GCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGC1686710.15717330147704942No Hit
GATGATCCTATGCTGTCGAGAAAAGCC1656280.15433773189843386No Hit
CGGATCTCTGGGCCATTACTGACGCTGAGGAGC1629540.15184600891019268No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCGG1546890.14414440438595427No Hit
GCACTCGTAGCTTAACGGATAGAGCATCT1528880.14246617211152557No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACTCG1512150.14090721453511287No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGCC1501350.1399008342706026No Hit
ACCGTTGGACGCGGGTTCGTTTCCCGCCATCTC1489810.1388254983213018No Hit
TGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGC1489180.13876679280587204No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTGC1452840.1353805095825106No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGC1435840.13379639249948516No Hit
TGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGCTTG1355920.1263491785421091No Hit
GTTTCTTGCCGGAGGTAGAGCACTGGATAG1337570.12463926392601986No Hit
GGGGCTATAGCTCAGTTGGTTAGAGCG1335700.12446501104688708No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAG1334590.1243615775197013No Hit
GTCCGGGTGGCGGAATGGCAGACGCGC1331750.12409693678347822No Hit
GCGGACGTGGCTCAGTTGGTAGAGCAT1323100.12329090073829176No Hit
GTTTCTTGCCGGAGGTAGAGCACTGGATAGG1301820.1213079588837752No Hit
AGGGTTCCTGGGTCAAGCTGATCTGCCCAGGGTAAGTCGG1277100.11900446627834055No Hit
GGAGAAGGTCTACGGTTCGTTTCCGTATTGGGG1257800.11720602747231756No Hit
TCGGGGTGATGGGGACTCACAGGAGACCGCCGGG1247490.11624530705314153No Hit
GGAGAAGGTCAACGGTTCGTTTCCGTTAAAGGG1212190.11295593452191811No Hit
AGCGGGTGTCGCAGGTTCGAATCCTGCCGGGGG1185780.1104949620417592No Hit
TCCAGGATGTCGCGGGTTCGAGTCCCGTTACTCACCC1170730.10909255250649255No Hit
TCCCCTGTAGCTCAATTGGCAGAGCATTCG1144870.10668283087313737No Hit
AGGGTTCCTGGGTCAAGCTGATCTGCCCAGGGTAAGTCG1144160.10661667068908162No Hit
CGCGGGGTGGAGCAGCTCGG1139640.10619548191171249No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)