FastQCFastQC Report
Thu 29 Jun 2023
ERR3949760.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949760.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158023893
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCC1875233811.866773842864383No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATCAC1621876010.26348591475341No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCC58558043.7056446900722797No Hit
TGGCCTATGGTGTAATTGGCAGCACGACG25899291.6389477254556688No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCG25379961.6060837078605574No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGC25328681.602838628966064No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGG23931521.5144241510364511No Hit
GCCCCAATAGCTCAGTCGGTAGAGCGTCTC20490291.2966577149190976No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGCCT20216811.2793514712360616No Hit
GGGGCGGTAGCTCAGCCGGTTAGAGCAGCG19781081.251777792868323No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCT19607161.2407718622651576No Hit
GGGCCTGTGGCGCAGTCTGGTAGCGCACCTC18022811.140511707302389No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGG17425751.1027288132940758No Hit
GCCCTTTTAGCTCAGTCGGTAGAGCGTCTC16487811.043374497804582No Hit
TCCGCTGTGCGCGTAGGAATATTGAGAAGGGCTGTCC14219200.8998132959551883No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGA14034630.8881334166346604No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCC10995310.6958004761976089No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCC10782700.6823461816625414No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGC9716580.614880434568208No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATC9219860.583447213264136No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTG9057460.5731702863439772No Hit
GTCCGGGTGGCGGAATGGCAGACGCGCTAG8778250.5555014392665292No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTCG7992620.5057855396588666No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGGG7600670.48098232841283056No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCG7365290.4660871125355708No Hit
TGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGCTT7360510.46578462663237896No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACTC7275960.4604341699137864No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA7020460.4442657288540537No Hit
ACCGGGACGGACGAACCTCTGGTGTGCCAG6718660.4251673511169605No Hit
ATGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGCTT6683350.4229328788906624No Hit
AGGAGAAGGTCAACGGTTCGTTTCCGTTAAAGGG6652600.42098697062222107No Hit
AGACCCCGGGACCTTTACTACAGTTTGATATTGG6486690.4104879253923962No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA6293750.39827837933343413No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCT6273860.39701970891199345No Hit
ACGGGGTCTAATACCGGATAACACCGG6233270.3944511099976508No Hit
ATGCAGGAGGTCAGGAGTTCATTTCTCCTTAGCTC6211440.39306967333098164No Hit
TCTTGGGCTGCACACGTGCTACAATGGCCGGT6108250.38653964815308023No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAACT5314150.33628775365001295No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAAC5279360.33408618783996163No Hit
CGGATCTCTGGGCCATTACTGACGCTGAGGAGC5221040.33039560669474205No Hit
GGGGCTATAGCTCAGTTGGTTAGAGCGC4950160.31325389509294016No Hit
ATGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGC4842030.306411258960694No Hit
TGGATGATCCTATGCTGTCGAGAAAAGC4726530.29910223765971894No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATCACC4720220.2987029309548778No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTG4716960.2984966330376382No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCG4652980.2944478782078859No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAAC4475260.2832014776398402No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAAC4393290.2780142873710876No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCG4185120.2648409630055121No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCC3943910.24957681557686975No Hit
ATGCGAAAGGTCCGGCGTAGAGGGTAAGACCCCCG3938000.24920282149991077No Hit
GATGATCCTATGCTGTCGAGAAAAGCC3811400.2411913747752057No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCCT3788160.2397207110952519No Hit
GCACTCGTAGCTTAACGGATAGAGCATCT3758500.2378437797377894No Hit
TCCCCTGTAGCTCAATTGGCAGAG3652750.2311517537414421No Hit
AGGAGAAGGTCTACGGTTCGTTTCCGTATTGGG3639260.23029808536611612No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACTC3576500.2263265340514045No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGAACT3566170.2256728354363476No Hit
GCGGACGTGGCTCAGTTGGTAGAGCAT3405240.2154889324236557No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTC3334820.21103264428500063No Hit
GGGGCTATAGCTCAGTTGGTTAGAGCG3142940.19889017668992623No Hit
TGCAGGAGGTCAGGAGTTCATTTCTCCTTAGCTC2997000.18965486440711848No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGC2887550.1827286966028612No Hit
GGCTCTGTGGAGCAGTTGGTTAGCTCGCC2883330.18246164837870435No Hit
ACCGTATGGATAGGCGAAGGACATGCGAAAGGT2659100.16827202200365993No Hit
CGGGACGGACGAACCTCTGGTGTGCCAG2641960.16718737589890917No Hit
AGGAGAAGGTCAACGGTTCGTTTCCGTTAAAGG2636100.16681654590043546No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAG2618780.1657205091131377No Hit
ACCGTTGGACGCGGGTTCGATTCCCGCCATCTC2598200.16441817440860035No Hit
AACGTTGGGAACTAGGTGTTGGCG2438080.15428552946736984No Hit
TGAAGTGGATGATCCTATGCTGTCGAGAAAAGC2433930.1540229109530924No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACCG2421850.15325846959105102No Hit
GTTTCTTGCCGGAGGTAGAGCACTGGATAG2403670.15210801065380664No Hit
ACCGTTGGACGCGGGTTCGTTTCCCGCCATCTC2384740.1509100905392832No Hit
GAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGC2383920.15085819965212477No Hit
AACCGTATGGATAGGCGAAGGACATGCGAAAGGT2306140.14593615916043784No Hit
GGACGATTAGCTCAGCGGGAGAGCACTTCC2225810.14085275066600214No Hit
CGCGGGGTGGAGCAGCTCGG2166260.13708433319004487No Hit
ACGGGGTCTAATACCGGATAACACCGGC2080290.13164401664247063No Hit
GTCCGGGTGGCGGAATGGCAGACGCGC1985450.12564239257160942No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCATTGCAG1940280.12278396406801596No Hit
GTTTCTTGCCGGAGGTAGAGCACTGGATAGG1936650.12255425196998533No Hit
TCCCCTGTAGCTCAATTGGCAGAGCATTCG1918410.12139999613855863No Hit
ATGTACACGGAGTCGACCCAACGCG1911850.12098486904129112No Hit
TGGGTGGAGCTGACCGGTACTAATAGGCCGAGGGC1831500.1159001949154613No Hit
ATGGGTGGAGCTGACCGGTACTAATAGGCCG1799730.11388973944592036No Hit
ATCCGCTGTGCGCGTAGGAATATTGAGAAGGGCTGTCC1780270.11265828009945306No Hit
TCCGCTGTGCGCGTAGGAATATTGAGAAGGG1732830.10965620243262833No Hit
CGAAAGGTCCGGCGTAGAGGGTAAGACCCCCGTAGC1732100.1096100068867434No Hit
GGAGAAGGTCTACGGTTCGTTTCCGTATTGGGG1672660.10584855038345373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)