FastQCFastQC Report
Sat 24 Jun 2023
ERR3949771_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949771_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126769305
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCC4113040632.44508282190235No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCC1789359514.115084877999449No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTAC17571831.3861265548470112No Hit
TAAGTGGATGATCCTATGCTGTCGAGAAAAGCC10033740.7914960171154997No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCC9255330.7300923516146121No Hit
GACGTGGATGATCCTATGCTGTCGAGAAAAGCC8872200.6998697358165685No Hit
GCCGTGGATGATCCTATGCTGTCGAGAAAAGCC6977540.5504124204199116No Hit
CGGGTGTAGTTTAATGGTAGAACATGAG6723710.5303894345717206No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGG5874150.46337321167770074No Hit
TGGATGATCCTATGCTGTCGAGAAAAGC5751020.45366029260789903No Hit
TGCATGATCCTATGCTGTCGAGAAAAGCC5685700.448507625722173No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAG5342620.4214442920547683No Hit
GGATGATCCTATGCTGTCGAGAAAAGCC5270430.415749695874723No Hit
GCAGTGGATGATCCTATGCTGTCGAGAAAAGCC5265270.41534265727811637No Hit
GAAGTGATCCTATGCTGTCGAGAAAAGCC4523840.3568561017195764No Hit
TCGATGATCCTATGCTGTCGAGAAAAGCC4429330.34940082695886043No Hit
TGAATGATCCTATGCTGTCGAGAAAAGCC4426310.349162598943017No Hit
TGGATGGATGATCCTATGCTGTCGAGAAAAGCC4157810.3279823928986595No Hit
TACGTGGATGATCCTATGCTGTCGAGAAAAGCC3518890.2775821796924737No Hit
TCCGTGGATGATCCTATGCTGTCGAGAAAAGCC3470680.2737792086183639No Hit
TCCTTGGATGATCCTATGCTGTCGAGAAAAGCC3431660.27070117644014846No Hit
GAAGTGGATGCTCCTATGCTGTCGAGAAAAGCC3306760.2608486336656969No Hit
GAAGTGGATGCTCCTCTGCTGTCGAGAAAAGCC3164740.24964560624513954No Hit
GAATTGGATGATCCTATGCTGTCGAGAAAAGCC3010470.23747625657488616No Hit
TAGATGATCCTATGCTGTCGAGAAAAGCC2953380.23297280047405797No Hit
TAATTGGATGATCCTATGCTGTCGAGAAAAGCC2920140.2303507146308012No Hit
TGGGTGATCCTATGCTGTCGAGAAAAGCC2793460.22035775931721013No Hit
TGGTTGATCCTATGCTGTCGAGAAAAGCC2755360.21735229991203314No Hit
TACTTGGATGATCCTATGCTGTCGAGAAAAGCC2699210.2129229942532224No Hit
TGGATGATCCTATGCTGTCGAGAAAGGCC2628040.20730885919111097No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGG2597320.20488555963922023No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCC2481950.1957847761333077No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCG2476950.1953903588885338No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGC2393280.18879018071448764No Hit
CGGATGATCCTATGCTGTCGAGAAAAGCC2124650.167599719821766No Hit
TCAGTGGATGATCCTATGCTGTCGAGAAAAGCC2094270.1652032406425199No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCC2086710.16460688176842178No Hit
GGGATGATCCTATGCTGTCGAGAAAAGCC1952800.15404359911888765No Hit
AGGATGATCCTATGCTGTCGAGAAAAGCC1851180.14602746303610326No Hit
AAAGTGGATGATCCTATGCTGTCGAGAAAAGCC1722110.13584597627951026No Hit
TGGATGATCCTATGCTGTCGAGAAGAGCC1692040.13347395096944012No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTT1676060.1322133934551428No Hit
TCATTGGATGATCCTATGCTGTCGAGAAAAGCC1642150.12953845570108632No Hit
TGGATGATCCTATGCCGTCGAGAAAAGCC1588200.1252826936299761No Hit
TGGATGACCCTATGCTGTCGAGAAAAGCC1584980.12502868892434174No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCG1536680.12121861833982604No Hit
TCCATGATCCTATGCTGTCGAGAAAAGCC1494060.11785660574537345No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCG1482440.11693998006851895No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGC1476620.11648087839560214No Hit
TCCCTGGATGATCCTATGCTGTCGAGAAAAGCC1351660.10662360261421326No Hit
TGGACGATCCTATGCTGTCGAGAAAAGCC1320170.10413956280662735No Hit
TGGATGATCCTATGCTGTCGGGAAAAGCC1309970.10333495162728865No Hit
TGGATGGTCCTATGCTGTCGAGAAAAGCC1292120.10192688206344588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)