FastQCFastQC Report
Sat 24 Jun 2023
ERR3949772_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949772_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109608582
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCC5461050949.82320544936892No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCC30184242.75382086413635No Hit
TGGATGATCCTATGCTGTCGAGAAAAGC13801251.2591395443834863No Hit
TGCATGATCCTATGCTGTCGAGAAAAGCC8769530.800076950178956No Hit
TCGATGATCCTATGCTGTCGAGAAAAGCC7962890.7264841725623272No Hit
GGATGATCCTATGCTGTCGAGAAAAGCC6828980.6230333314593925No Hit
TGGACGATCCTATGCTGTCGAGAAAAGCC5759310.5254433453030165No Hit
TGGAAGATCCTATGCTGTCGAGAAAAGCC5594510.5104080262620312No Hit
TAGATGATCCTATGCTGTCGAGAAAAGCC4459220.4068312826088745No Hit
TGAATGATCCTATGCTGTCGAGAAAAGCC4430730.4042320335829178No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCC4399740.401404700226849No Hit
TGGTTGATCCTATGCTGTCGAGAAAAGCC4254990.38819861751336227No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTAC3853330.3515536767002423No Hit
AGGATGATCCTATGCTGTCGAGAAAAGCC3598190.32827630230632854No Hit
TGGGTGATCCTATGCTGTCGAGAAAAGCC3362510.3067743363380068No Hit
GAAGTGATCCTATGCTGTCGAGAAAAGCC3189630.2910018487420994No Hit
TGGATGATCCCATGCTGTCGAGAAAAGCC3137450.286241272604001No Hit
TGGATGATCCTATGCCGTCGAGAAAAGCC3120030.2846519809917804No Hit
CGGATGATCCTATGCTGTCGAGAAAAGCC2844710.25953350988520224No Hit
GACGTGGATGATCCTATGCTGTCGAGAAAAGCC2836290.2587653218613849No Hit
TGGATGATCCTATGCTGTCGAGAAAGGCC2827790.25798983513900403No Hit
GGGATGATCCTATGCTGTCGAGAAAAGCC2581750.235542687706698No Hit
TAAGTGGATGATCCTATGCTGTCGAGAAAAGCC2420910.22086865424461016No Hit
TGGATGACCCTATGCTGTCGAGAAAAGCC2405680.21947916450556765No Hit
TGGATGATCCTATGCTGTCGAGAAATGCC2245550.20486990699323163No Hit
TGGATGATCCTATGCTGTCGAGAAGAGCC2087780.19047596108852133No Hit
TGGATGATCCTATGCTGTCGAGATAAGCC1990320.1815843215634338No Hit
TGGATGGTCCTATGCTGTCGAGAAAAGCC1793080.1635893802549147No Hit
TCCATGATCCTATGCTGTCGAGAAAAGCC1780390.16243162419526602No Hit
GCCGTGGATGATCCTATGCTGTCGAGAAAAGCC1728380.15768655779161528No Hit
TGGATGGATGATCCTATGCTGTCGAGAAAAGCC1668560.1522289559406945No Hit
TGGATGATCCTATGCTGCCGAGAAAAGCC1554470.1418200994517017No Hit
TGGATGATCCTATGCTGTTGAGAAAAGCC1528310.13943342502140937No Hit
TGGATGATCCTATGCTGTCGGGAAAAGCC1474240.13450041712974628No Hit
GCAGTGGATGATCCTATGCTGTCGAGAAAAGCC1465790.1337294920939676No Hit
TGGATGATCCTACGCTGTCGAGAAAAGCC1411860.12880925692479078No Hit
GAATTGGATGATCCTATGCTGTCGAGAAAAGCC1403550.1280511046114984No Hit
TGGATGATCCTATGCTGTCGAGGAAAGCC1403290.12802738384116674No Hit
TGGATGATCCTATGCTGTCGAGAAAAG1349050.12307886621505605No Hit
TGGATGATCCTATGCTGTCGTGAAAAGCC1288280.1175345923186927No Hit
TGGATGATCCTATGCTGTCGAGAAAAACC1282550.11701182303407592No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAG1207660.11017932884123981No Hit
TGGATGATCCTATGCTGTCGAGAGAAGCC1191850.1087369235376113No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)