FastQCFastQC Report
Thu 29 Jun 2023
ERR3949773_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949773_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157083409
Total Bases4.8 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCC2912894218.543614621961762No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCC1859887811.840128832447226No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAG74416794.737406099965656No Hit
CGGGTGTAGTTTAATGGTAGAACATGAG68067474.333205551962525No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCG36661362.333878557473883No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGG34437992.192337829897746No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCC22737931.447506782845539No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTAC22644231.441541798981457No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGG16831821.071521181463537No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCC15511840.9874906649116585No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTGG12423270.7908709187741145No Hit
CGGGACGTGGCGCAGCTTGGTAGCGCACTTGAC12355610.7865636529444048No Hit
CCCCTGTAGCTCAATTGGCAGAGCAGCC12072080.7685140064664627No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGG10477050.6669736840254084No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGC10114100.6438681248635238No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGT9884710.629265054974711No Hit
CCCTGTAGCTCAATTGGCAGAGCAGCCGG8663580.5515273735878752No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGG7567160.4817287865200328No Hit
GGGTGTAGTTTAATGGTAGAACATGAG7194570.4580095406511072No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCC7151990.45529887882685305No Hit
GGATGATCCTATGCTGTCGAGAAAAGCC6982310.4444969742157811No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCG6940870.44185888530086587No Hit
TGGCTGATCCTATGCTGTCGAGAAAAGCC6867210.4371696567904253No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCG5984550.3809791268280917No Hit
CCCCTGTAGCTCAATTGGCAGAGCAGCCGG5757760.3665415741009288No Hit
CCTCATCGTCTAGTGGCCCAGGACGCCG5218900.33223750574448No Hit
CCCTCATCGTCTAGTGGCCCAGGACGCCG4917410.31304451764221647No Hit
ACGTGGGCAATCTGCCCTGCACTCTGGG4444140.2829159379906251No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCGG3598530.2290840275818053No Hit
CGGGGTGGAGCAGCTCGGTAGCTCGCTGGG3198490.2036173024485355No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCAATC3114690.19828255700765954No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTG3036050.19327629947221223No Hit
CCCTGTAGCTCAATTGGCAGAGCAGCC2975880.18944585038894846No Hit
GGAGTGGATGATCCTATGCTGTCGAGAAAAGCC2845490.1811451647321965No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTG2675690.17033562086750995No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAGC2552420.1624881975918921No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGC2523530.16064904728417245No Hit
CGGGTGTAGTTTAATGGTAGAACATGAGC2443750.15557021683938627No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGA2386750.15194157137244202No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAGGCC2323310.14790295262817985No Hit
CCCCTGTAGCTCAATTGGCAGAGCAGCCG2218090.14120460041709434No Hit
GTGGATGATCCTATGCTGTCGAGAAAAGCC2153750.1371086872707225No Hit
GCGGGGTGGAGCAGCTCGGTAGCTCGCTGGG2109910.13431781328351486No Hit
CCCCTGTAGCTCAATTGGCAGAGCAGC2004710.12762073428136514No Hit
CGGGTGTAGTTTAATGGTAGAACAT1921510.12232418510856229No Hit
GAGGTGGATGATCCTATGCTGTCGAGAAAAGCC1894390.12059771379165829No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGC1809920.11522031585143407No Hit
ATGTACACGGAGTCGACCCAACGCG1713670.10909299784804134No Hit
GGGTCGTAGCTCAATTGGTAGAGCACTGG1684620.10724366186883555No Hit
ACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGA1666650.10609968364004628No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTC1657120.10549299958215193No Hit
AGGGTCGTAGCTCAATTGGTAGAGCAC1654390.10531920656241933No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCG1623840.10337437991303079No Hit
GTAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCAATC1587010.1010297656578105No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)