FastQCFastQC Report
Sun 2 Jul 2023
ERR3949774_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949774_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161666872
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCC2592307416.034870768081664No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCC138117668.543349561436434No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAG108379096.703852722529326No Hit
CGGGTGTAGTTTAATGGTAGAACATGAG87323475.401444892185457No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGG39119572.4197641431449233No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCG31179011.9285961071851505No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCC20753951.2837478540439626No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTGG18976481.173801395749155No Hit
CGGGACGTGGCGCAGCTTGGTAGCGCACTTGAC18719661.1579156427298227No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTAC18512131.1450787518175027No Hit
ACGTGGGCAATCTGCCCTGCACTCTGGG17707921.0953338665450274No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGG17492831.082029347360664No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCC14492460.8964396861714501No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGG13120180.8115564950127816No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGT13073960.8086975295717974No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGG12573970.7777703523576556No Hit
ACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGA11481120.7101714691430412No Hit
CCCCTGTAGCTCAATTGGCAGAGCAGCC10602320.6558127752975885No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCG10477360.6480833005787358No Hit
GGGTGTAGTTTAATGGTAGAACATGAG7805060.48278660330608736No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGC7460660.46148353757967187No Hit
CCCTGTAGCTCAATTGGCAGAGCAGCCGG7154830.44256624201895856No Hit
ATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACG6513440.4028926841610444No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCG6037870.3734760204923121No Hit
CCCCTGTAGCTCAATTGGCAGAGCAGCCGG5685650.3516892440400529No Hit
GGATGATCCTATGCTGTCGAGAAAAGCC5552890.3434772957071873No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCC4943860.3058053847915113No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCGG4542950.2810068595871639No Hit
TGGGGTGTTGGGGACTCACGGGAGACCGCCGGGGT4536140.2805856230087757No Hit
AGGACTCTCAAGGAGTCAGGGAGAACTCATCTCGGGG4478460.2770177924887419No Hit
CCTCATCGTCTAGTGGCCCAGGACGCCG4240440.2622949245903638No Hit
CGGGGTGGAGCAGCTCGGTAGCTCGCTGGG4182490.2587103930606142No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTG4182070.2586844137121673No Hit
CGGGTGTAGTTTAATGGTAGAACATGAGC3902850.2414130954423365No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTG3858940.23869701641781008No Hit
CCCTCATCGTCTAGTGGCCCAGGACGCCG3579640.22142074970065606No Hit
ATCCTTGAAGGTTGAAAGCTCCGGCGGTG3402480.21046241310340935No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAGC3319740.20534448145938022No Hit
AGGATCCACTCAGGAGAGAACGAAGTTTTGACT3164900.1957667616653089No Hit
CGGGTGTAGTTTAATGGTAGAACAT3156930.1952737726007342No Hit
CCCCTGTAGCTCAATTGGCAGAGCAGCCG3056850.1890832649993995No Hit
GCGGGGTGGAGCAGCTCGGTAGCTCGCTGGG2802320.17333916128469412No Hit
ATGGGCTGTCACCCGCTGCTGAACACATAGG2740390.1695084445006148No Hit
GGGGTGTTGGGGACTCACGGGAGACCGCCGGGGT2688580.1663037063029215No Hit
GGAGTGGATGATCCTATGCTGTCGAGAAAAGCC2460650.15220496132318315No Hit
TGGCTGATCCTATGCTGTCGAGAAAAGCC2351840.1454744544077033No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGC2296900.14207610820848937No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATCAC2277110.1408519860519105No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGA2191170.13553611651495304No Hit
GTGGATGATCCTATGCTGTCGAGAAAAGCC2174840.13452601470510298No Hit
CCCTGTAGCTCAATTGGCAGAGCAGCC2141160.13244271838203192No Hit
GGGTCGTAGCTCAATTGGTAGAGCACTGG2064030.12767179660654288No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCAATC1935630.11972953865279214No Hit
AAGGAGTCAGGGAGAACTCATCTCGGGG1877590.11613944011980389No Hit
AACCCGGATTACGGGTCAACGTTAGAACATCAA1821010.1126396507504642No Hit
AAACGTTGGGAACTAGGTGTTGGCG1762110.10899635640875145No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAGGCC1736130.10738934814053928No Hit
GCGGGTGTAGTTTAATGGTAGAACAT1735130.10732749254899916No Hit
AGGGTCGTAGCTCAATTGGTAGAGCAC1730760.10705718361396885No Hit
GAGGTGGATGATCCTATGCTGTCGAGAAAAGCC1674900.10360193027053805No Hit
AGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGG1646930.10187182937516104No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)